In vitro Evaluation of the Effects of Inula viscosa’s Different Extracts on Wound Healing and Oxidative Stress in Mouse L929 Fibroblast Cell Line

Abstract

This study evaluated the effects of extracts prepared using two different methods (decoction extraction and soxhlet ethyl acetate/hexane extraction) from Inula viscosa on H2O2-induced oxidative stress and wound healing model in mouse L929 fibroblast cells. The cytotoxic effect started to disappear statistically (p<0.05) at concentrations of Soxhlet ethyl acetate/hexane extracts (SoxEHE) in and below 0.1 mg/ mL, while the same effect was observed at concentrations of decoction extracts (DE) in and below 0.2 mg/mL. Therefore, 0.2 and 0.02 mg/ mL concentrations of DE, and 0.1 and 0.01 mg/mL concentrations of SoxEHEs were used. While cell migration was positively affected in all research concentrations, statistically significant results (p<0.05) were obtained from 0.2 mg/mL of DE and 0.1 mg/mL of SoxEHE extracts. Malondialdehyde (MDA) levels were found to be statistically (p <0.05) decreased, but COL1A1 levels were higher in cell lines treated with oxidative stress + extract than in the cell line treated only with H2O2, and reduced glutathione (GSH) levels were higher in cell lines only treated only with extract than in oxidative stress-induced cell lines. Consequently, it has been observed that the extracts have positive effects on migration and oxidative stress. T herefore, I. viscosa may serve as a new therapeutic agent for wound healing.