TRDizin:
Investigation of Antimicrobial Activities and 16S rRNA Sequences of Actinomycetes Isolated from Karst Caves in the Eastern Black Sea Region of Türkiye

dc.contributor.authorTüfekci, E.F.
dc.contributor.authorUzun, Ü.
dc.contributor.authorErtunga, N.S.
dc.contributor.authorBiber, A.
dc.contributor.authorHıdıroğlu, İ.A.
dc.contributor.authorTekkılıc, İ.
dc.contributor.authorAltay, B.
dc.contributor.authorKılıc, A.O.
dc.date.accessioned2023-12-08T05:50:32Z
dc.date.available2023-12-08T05:50:32Z
dc.date.issued2023
dc.description.abstractConsidering that most antibiotics originate from actinomycete group bacteria, especially the Streptomyces genus, it is predicted that novel actinomycetes isolated from extreme environments such as caves may bring novel antibiotics to the medical world. The study aimed to screen the antimicrobial activity of actinomycetes isolated from the three karst caves in Türkiye and to identify selected isolates with antimicrobial activity by molecular methods. One hundred seventy-nine actinomycetes isolated from Akçakale, Kırklar (Altıntaş), and Köprübaşı Caves in Gümüşhane province in the Eastern Black Sea Region of Türkiye were included in the study. The antimicrobial activity of isolates was investigated using the modified cross-streak agar method against seven Gram-negative bacteria, three Gram-positive bacteria, and one yeast strain. Fifty-three isolates (29.6%) had antimicrobial activity against at least one of the tested microorganisms. The rate of isolates exhibiting antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Chromobacterium violaceum, Klebsiella pneumoniae, Salmonella Typhimurium, Escherichia coli, Acinetobacter haemolyticus, Pseudomonas aeruginosa, Enterobacter aerogenes, and Candida albicans was 21.2%, 20.0%, 16.8%, 12.8%, 3.4%, 2.8%, 2.2%, 1.1%, 0.6%, 0.6%, and 0.6%, respectively. An actinomycete isolate, TRMS 124, showed antimicrobial activity against ten test microorganisms. The 16S ribosomal RNA (16S rRNA) sequencing was performed for the identification and phylogenetic analysis of 26 isolates randomly selected among actinomycetes that exhibited antimicrobial activity against at least three test microorganisms. As a result, it was determined that 24 isolates showed homology with various Streptomyces species and two isolates with Embleya scabrispora and Couchioplanes caeruleus, respectively. These results showed that karst caves could be good sources for isolating actinomycetes with the potential to produce antimicrobial compounds.
dc.identifier.citationTüfekci, E., Tekkılıç, İ., Altay, B., Kilic, A., Uzun, Ü., Sağlam Ertunga, N., Biber, A., Hıdıroğlu, İ. (2023). Investigation of Antimicrobial Activities and 16S rRNA Sequences of Actinomycetes Isolated from Karst Caves in the Eastern Black Sea Region of Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 26(6), 1277-1290
dc.identifier.doi10.18016/ksutarimdoga.vi.1226184
dc.identifier.eissn2619-9149
dc.identifier.endpage1290
dc.identifier.issue6
dc.identifier.orcid0000-0001-5051-2694
dc.identifier.orcid0000-0002-2522-7370
dc.identifier.orcid0000-0003-2025-7869
dc.identifier.orcid0000-0002-5506-0866
dc.identifier.orcid0000-0002-0585-2865
dc.identifier.orcid0000-0002-2104-2192
dc.identifier.orcid0000-0001-8431-2703
dc.identifier.orcid0000-0002-3030-6006
dc.identifier.startpage1277
dc.identifier.trdizin1207214
dc.identifier.trdizinauthorid997873
dc.identifier.trdizinauthorid997877
dc.identifier.trdizinauthorid997878
dc.identifier.trdizinauthorid786355
dc.identifier.trdizinauthorid980574
dc.identifier.trdizinauthorid997874
dc.identifier.trdizinauthorid997875
dc.identifier.trdizinauthorid997876
dc.identifier.urihttps://search.trdizin.gov.tr/publication/detail/1207214/investigation-of-antimicrobial-activities-and-16s-rrna-sequences-of-actinomycetes-isolated-from-karst-caves-in-the-eastern-black-sea-region-of-turkiye
dc.identifier.urihttps://hdl.handle.net/20.500.12597/17933
dc.identifier.volume26
dc.language.isoeng
dc.relation.ispartofKahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject16S ribosomal RNA, Actinomycetes, Antimicrobial activity, Cave, Streptomyces
dc.titleInvestigation of Antimicrobial Activities and 16S rRNA Sequences of Actinomycetes Isolated from Karst Caves in the Eastern Black Sea Region of Türkiye
dc.typearticle
dspace.entity.typeTrdizin

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