Expression Patterns of Bone Morphogenetic Proteins (BMP) in PGF2a Induced Luteolysis in Ovine Corpus Luteum

Abstract

The aim of the present study was to assess the mRNA expression of the BMP system in the induced luteolysis of ovine corpus luteum (CL). CL samples were obtained from the induced luteolysis groups. In this study, we used the induced luteolysis model, the ewes were treated with PGF2α on the 12th day of the cycle, and luteal tissues were collected at 0 hours (without PGF2α treatment, n= 4), 4 h (PG4, n=4), and 16 h (PG16, n=4) after injection. mRNA expression levels of BMP components were quantified using RT-qPCR. SDHA was used as a reference control for the normalization of qPCR data. Expression of BMP4 mRNA was found to be greater in PG16 than in PG4 (P<0.01). Expression of BMP6 was shown to be greater in PG16 than in C12 (P<0.01) but did not differ between C12 and PG4 (P>0.05). Expression of BMP6 and BMPR1A mRNA was found to be higher in PG16 than in PG4 (P<0.05). While expression of BMPR1B mRNA was greater in PG16 than in PG4 and C12 (P<0.0001), it was shown to be lower in PG4 than in C12 (P<0.05). Although BMPR2 mRNA was found to be lower in PG16 and PG4 than in C12, this was not statistically significant (P>0.05). We may suggest that expression patterns of some BMP genes regulate luteolysis of ovine CL.