Chemical Screening and Antibacterial Activity of Crude Extracts of Chlorella sp. in Culture

Abstract

In this study, the antibacterial activity of methanol and acetone extracts of Chlo-rella sp. was examined. The chemical contents of the extracts were clarified by GC-MS analysis. Antibacterial activity of Chlorella sp. extracts was determined as a minimum inhibitory concentration by broth microdilution method against Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus au-reus ATCC 29213. It was found that methanol and acetone extracts of Chlorella sp. showed antibacterial activity against B. subtilis ATCC 6633 (625 µg/ml and 1250 µg/ml, respectively), E. faecalis ATCC 29212 (>5000 µg/ml and 1250 µg/ ml, respectively), E. coli ATCC 25922 (>5000 µg/ml), P. aeruginosa ATCC 27853 (>5000 µg/ml), S. aureus ATCC 29213 (2500 µg/ml) at the specified concentra-tions. In the chemical analysis of the extracts, it was determined that the fatty acids were in high amounts, 33,22%, and 40,41%, respectively, in the methanol and acetone extracts. Among the alternative methods to show activity against pathogenic microorganisms, algae can be a good natural resource. This study showed that Chlorella sp. contains high fatty acids and has the potential as an antibacterial agent of natural origin.