Scopus:
Relative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheep

dc.contributor.authorAlak I.
dc.contributor.authorHitit M.
dc.contributor.authorKose M.
dc.contributor.authorKaya M.S.
dc.contributor.authorUcar E.H.
dc.contributor.authorAtli Z.
dc.contributor.authorAtli M.O.
dc.date.accessioned2023-04-12T01:25:16Z
dc.date.available2023-04-12T01:25:16Z
dc.date.issued2020-05-01
dc.description.abstractThe aim of this study was to investigate relative abundance and localization of ISG15 mRNA transcript in intra-uterine (trophoblast, endometrium) and extra-uterine (hypothalamus, anterior pituitary, corpus luteum) tissues before and during the period of conceptus implantation. Multiparous ewes (n = 16) were randomly allotted into four groups: pregnant or estrous cyclic on days of 12 and 16 (n = 4 per group) following estrus. Relative abundances of ISG15 mRNA transcript were determined in the endometrium, corpus luteum, hypothalamus, and anterior-pituitary using real time quantitative PCR. Localization of ISG15 mRNA transcript was evaluated using in situ hybridization. The presence of ISG15 mRNA transcript was only visualized in intra-uterine tissues including the endometrium and trophoblast on day 12 of pregnancy. The ISG15 mRNA transcript was detected in all tissues evaluated on day 16 of pregnancy. The abundance of ISG15 mRNA transcript was greater in the endometrium on day 12 of pregnancy than at other days when evaluations occurred while in all other tissues except the hypothalamus there were large abundances of ISG15 mRNA on day 16 of pregnancy. It is concluded that the ISG15 mRNA transcript is only present in intra-uterine tissues before conceptus implantation. The ISG15 mRNA transcript, however, is present in extra-uterine tissues of ewes during implantation probably due to an increased amount of interferon-tau in blood circulation that is produced by the developing embryo. Results also indicate, for the first time, that pregnancy is associated with an intra-hypothalamus and anterior pituitary increased abundance of ISG15 mRNA transcript in ewes.
dc.identifier.doi10.1016/j.anireprosci.2020.106347
dc.identifier.issn03784320
dc.identifier.pubmed32414457
dc.identifier.scopus2-s2.0-85083398463
dc.identifier.urihttps://hdl.handle.net/20.500.12597/4753
dc.relation.ispartofAnimal Reproduction Science
dc.rightsfalse
dc.subjectAnterior-pituitary | Ewes | Hypothalamus | ISG15 mRNA | Pregnancy
dc.titleRelative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheep
dc.typeArticle
dspace.entity.typeScopus
oaire.citation.volume216
person.affiliation.nameDicle Üniversitesi
person.affiliation.nameKastamonu University
person.affiliation.nameDicle Üniversitesi
person.affiliation.nameAnkara Yildirim Beyazit University
person.affiliation.nameAdnan Menderes Üniversitesi
person.affiliation.nameDicle Üniversitesi
person.affiliation.nameDicle Üniversitesi
person.identifier.scopus-author-id57216389117
person.identifier.scopus-author-id57189365255
person.identifier.scopus-author-id35778206100
person.identifier.scopus-author-id55057679700
person.identifier.scopus-author-id57078297100
person.identifier.scopus-author-id57216390087
person.identifier.scopus-author-id8605008500
relation.isPublicationOfScopus0518935c-dc9e-45d3-888f-a9b65f3aee4f
relation.isPublicationOfScopus.latestForDiscovery0518935c-dc9e-45d3-888f-a9b65f3aee4f

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