Scopus:
A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation

dc.contributor.authorCeylan, H.K.
dc.contributor.authorKırbay, F.Ö.
dc.contributor.authorYazgan, İ.
dc.contributor.authorElibol, M.
dc.date.accessioned2024-02-28T06:03:57Z
dc.date.available2024-02-28T06:03:57Z
dc.date.issued2024
dc.description.abstractVascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the circulating system are signs of pathology-associated diseases. Therefore, its precise and selective detection is critical for clinical applications to monitor the progression of the pathology. In this study, an optical immunoassay biosensor was developed as a model study for detecting recombinant VEGF165. The VEGF165 sample was purified from recombinant Kluyveromyces lactis GG799 yeast cells. Indirect ELISA was used during the detection, wherein iron oxide nanoparticles (FeNPs) were utilized to obtain optical signals. The FeNPs were synthesized in the presence of lactose p-amino benzoic acid (LpAB). VEGF165 antibody was conjugated to the LpAB-FeNPs through EDC/NHS chemistry to convert the iron oxide nanoparticles into VEGF165 specific probes. The specificity of the prepared system was tested in the presence of potential serum-based interferents (i.e., glucose, urea, insulin, C-reactive protein, and serum amyloid A), and validation studies were performed in a simulated serum sample. The proposed immunoassay showed a wide detection range (0.5 to 100 ng/mL) with a detection limit of 0.29 ng/mL. These results show that the developed assay could offer a sensitive, simple, specific, reliable, and high-throughput detection platform that can be used in the clinical diagnostics of VEGF. Graphical Abstract: (Figure presented.)
dc.identifier10.1007/s00604-024-06228-0
dc.identifier.doi10.1007/s00604-024-06228-0
dc.identifier.issn00263672
dc.identifier.issue3
dc.identifier.pubmed38353782
dc.identifier.scopus2-s2.0-85185101323
dc.identifier.urihttps://hdl.handle.net/20.500.12597/19089
dc.identifier.volume191
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMicrochimica Acta
dc.relation.ispartofseriesMicrochimica Acta
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHuman vascular endothelial growth factor, Immunoassay, Indirect ELISA, Iron oxide nanoparticle, Optical detection
dc.titleA colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation
dc.typearticle
dspace.entity.typeScopus
oaire.citation.issue3
oaire.citation.volume191
person.affiliation.nameTokat Gaziosmanpaşa Üniversitesi
person.affiliation.nameEge Üniversitesi
person.affiliation.nameKastamonu University
person.affiliation.nameEge Üniversitesi
person.identifier.orcid0000-0003-0365-2760
person.identifier.scopus-author-id57223841129
person.identifier.scopus-author-id57201278315
person.identifier.scopus-author-id24554371300
person.identifier.scopus-author-id6701773932

Files