Phytotoxicity, Toxicity on Brine Shrimp and Insecticidal Effect of Aytac & Anderb. Growing in Turkey.

Abstract

The aim of this study was to investigate the probable toxicity on brine shrimp, phytotoxicity, and insecticidal activity of Aytac & Anderb.The MeOH (80%) extract obtained from the whole plant of was fractionated through subsequent solvent extractions in increasing polarity with -hexane, chloroform, and -butanol. The MeOH (80%) extract and all fractions of were evaluated for their biologic activities using screening bioassays such as brine shrimp lethality test and phytotoxicity against , as well as insecticidal activity against and .The findings showed that the -hexane and chloroform fractions of the plant had significant phytotoxic activities with 100% growth inhibition (GI) at 1000 μg/mL against . Moreover, the MeOH (80%) extract (53% GI) and -butanol fraction (46.6% GI) of the plant had moderate phytotoxic activities at 1000 μg/mL. Otherwise, no samples had toxicity on the brine shrimps. In addition, the remaining water fraction had low insecticidal activity with 20% mortality against .Our results show that the n-hexane and chloroform fractions of had potential phytotoxic effects.