Pubmed İndeksli Yayınlar

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12597/3195

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Absorption, Steady-State Fluorescence and Solvent Effect Studies of Fluorescent Haloarchaeal Bacterioruberin
(2025) Zurnacı, M.; Kesbiç, F.I.
Fluorescence characterization of halophilic archaeal C50 carotenoid-bacterioruberin extracts was investigated using UV/Vis and steady-state fluorescence spectrophotometry in solvents with different polarity. Different extracts showed maximum absorption and fluorescence wavelengths between 369-536 nm and 540-569 nm. Stokes' shifts varied between 50-79 nm depending on the solvent. In particular, water extract showed significant Stokes' shifts with the increase in polarity index; however, fluorescence wavelength (band position) and shape were found to be independent of polarity. According to the obtained results, it is thought that bacterioruberin will be a new alternative material with increasing applications in optics, biosensor/chemosensor, biotechnology, analytical chemistry and nanotechnology due to its fluorescence properties.
Increasing microplastics pollution: An emerging vector for potentially pathogenic bacteria in the environment
(2025) Tavşanoğlu, Ü.N.; Akca, G.; Pekmez, T.; Kankılıç, G.B.; Çırak, T.; Çağan, A. S.; Kotiloğlu, S. Ö.; Grossart, H.P.
Microplastics (MP), plastic particles <5 mm, are of global concern due to their worldwide distribution and potential repercussions on ecosystems and human well-being. In this study, MP were collected from the urbanized Susurluk basin in Türkiye to evaluate their vector function for bacterial biofilms, both in the wet and dry seasons. Bacterial biofilms were predominantly found on polyethylene (PE), polypropylene (PP), and polystyrene (PS), which constitute the most common MP types in the region. Specific potentially pathogenic bacterial genera, including Pseudomonas sp., Comamonas sp., Salmonella spp., and Shigella spp., were prevalent on MP surfaces. Notably, PE and PP harboured numerous genera of potential human and/or animal origin such as Staphylococcus, Proteus, Escherichia, Enterococcus, and Enterobacter. Water quality played a pivotal role in bacterial biofilm formation on MP. Higher salinity in estuarine areas reduced bacterial abundance on MP, while the more polluted freshwater Nilüfer Stream harboured a higher abundance of total bacteria, particularly of potentially pathogenic strains. Seasonal variations, ambient water conditions, and polymer type are all factors that could influence bacterial colonization on MPs. This catchment-wide evaluation, which includes various habitat types (lentic and lotic systems), the enrichment of cultivable viable bacteria on microplastics (MPs) - a key factor in the spread of pathogens - has significant implications for both environmental and public health. Unlike controlled laboratory experiments or in-situ studies with various particles, this study emphasized the dynamic and complex nature of bacterial strains on MPs, which varied depending on seasonal dynamics and antropogenic impacts in open systems. Further research is needed to thoroughly investigate to fully explore the complex interactions among MPs, microbial communities, and their ecological roles, especially in the context of changing environmental factors across entire river catchments.
The relationship between sexual health literacy and sexual function of women with diabetes mellitus: a cross-sectional study
(2025) Çakmak, B.; Abay, H.; Doğanay, C.A.; Çelik, N.; Özel, Y.; Üstün, Y.
Background: Sexual dysfunction (SD) is a complication of poorly managed diabetes mellitus (DM). To prevent SD, patients should develop sexual health literacy (SHL). Objective: This study investigated the relationship between SHL and SD in women with DM. Methods: This cross-sectional study was performed between 1 October 2023 and 1 June 2024. The sample comprised 400 participants. The inclusion criteria were (1) being 18-65 years of age, (2) having been diagnosed with DM, and (3) having a sex partner. Data were collected using a personal information form, the Female Sexual Function Index (FSFI), and the Sexual Health Literacy Scale (SHLS). Outcomes: The data were analyzed using the Mann-Whitney test, Kruskal-Wallis H test, Spearman correlation coefficients, and binary logistic regression. Results: Over half of the participants experienced SD (68,2%). Participants with higher education, those whose partners had higher education, those who did not have any chronic disease other than DM, and those who did not take hormone replacement therapy had a lower rate of SD (P < 0.05). Participants with higher income, those who used family planning, those with DM I, and non-menopausal participants had lower SD and higher SHL (P < 0.05). Insulin-only participants had higher SD and lower SHL than those who were on other types of medications (P < 0.05). There was a significant negative correlation between scale scores (FSFI and SHLS) and age (r = -0.388; P < 0.001 r = -0.326; P < 0.001, respectively), age of partner (r = -0.383; P < 0.001, r = -0.274; P < 0.001, respectively), duration of romantic relationship (r = -0.326; P < 0.001, r = -0.328; P < 0.001, respectively), number of children (r = -0.109; P < 0.001, r = -0.290; P < 0.001, respectively), and duration of DM (r = -0.254; P < 0.001, r = -0.125; P < 0.013, respectively). There was a significant positive correlation between scale scores (FSFI and SHLS) and number of sexual intercourse (r = 0,493; P < 0.001, r = 0.127; P < 0.011, respectively). A one-unit increase in DM duration resulted in a 3.7% increase in SD rate (OR = 1.037). A one-unit increase in the number of sexual intercourses reduced the SD rate by 35.5% (OR = 0.645). Clinical implication: The data show that the prevalence of SD in diabetic women is directly affected by the number of sexual intercourses per week, menopausal status, and duration of DM. Strengths and limitations: This is the first study to examine the relationship between SHL and SD in women with DM. Second, the results are sample-specific and cannot be generalized to all women with DM. Conclusion: Healthcare professionals should ensure that women with DM have high levels of SHL to prevent SD and improve their quality of sexual life.
Exploring in vitro efficacy of rCHAPk with antibiotic combinations, and promising findings of its therapeutic potential for clinical-originated MRSA wound infection
(2025) Tasdurmazli, S.; Cinar, I.; Karamese, M.; Karamese, S.A.; Cadirci, E.; Melo, Luís D R; Ozbek, T.
The increasing threat of antimicrobial-resistant bacteria, particularly Staphylococcus aureus, which rapidly develops multidrug resistance and commonly colonizes wound surfaces, demands innovative strategies. Phage-encoded endolysins offer a dual-purpose approach as topical therapies for infectious skin wounds and synergistic agents to reduce high-dose antibiotic dependence. This study explores recombinant CHAPk (rCHAPk), efficiently synthesized within 3 h, displaying broad-spectrum antibacterial activity against 11 Gram-positive strains, including resistant variants, with rapid bactericidal kinetics. Application of 10 μg of rCHAPk reduced OD by 0.4 within 5 min against a clinical methicillin-resistant S. aureus (MRSA) strain. Combining rCHAPk (1.875 μg/mL) with oxacillin/vancomycin lowered their minimum bactericidal concentrations to 1 μg/mL from initial values over 64 μg/mL and 32 μg/mL, respectively, with a fractional inhibitory concentration index below 0.1. rCHAPk retained efficacy after one year of refrigerated storage. In in vivo experiments, rCHAPk outperformed commercial fucidin therapy in MRSA-induced murine wound models over two weeks, enhancing wound healing by modulating pro-inflammatory cytokine responses and the proliferative phase. This study, for the first time, investigates rCHAPk's in vitro combination with antibiotics and wound healing parameters, highlighting its potential as a potent antibacterial agent synergizing with antibiotics to address antibiotic-resistant bacterial wound infections.
The potential protective effects of curcumin on the diabetic ovary: Experimental and molecular approaches
(2024) Tufekci, K.K.; Altun, G.; Kipanyula, M.J.; Kaplan, S.
Diabetes mellitus (DM) causes numerous systemic diseases in animals and humans. This may also lead to reproductive problems among individuals of reproductive age. Detrimental effects such as apoptosis in ovarian granulosa cells, degradation of communication proteins, decreased oocyte quality, delayed meiotic maturation, and atrophy are among the increasing evidence that chronic hyperglycemia causes reproductive problems. Numerous studies have reported that the antidiabetic properties of the antioxidant curcumin may be due to its inhibition of oxidative stress, inflammation, and insulin resistance. There are also data indicating that curcumin reduces the risk of DM and its associated symptoms. This review discusses the protective or curative properties of curcumin in the treatment of DM-related problems in the ovary and seeks to elucidate potential underlying mechanisms. While the use of curcumin as a supportive/therapeutic agent has been introduced for the reduction of reproductive problems that may be caused by uncontrolled DM, more studies on this subject are needed.
High-Sensitive Cardiac Troponin I and Th1/Th2 Polarization in Queens With Pyometra
(2025) Safak, T.; Saat, N.; Yilmaz-Koc, O.; Turanli, M.; Ayalp-Erkan, A.; Risvanli, A.
Objectives: To determine T helper (Th)1 and Th2 cytokine polarization, as well as high-sensitive cardiac troponin I (hs-cTnI) levels, in cats with pyometra. Methods: We used 40 queens in the study. A total of 20 out of these 40 queens were diagnosed with the pyometra group (PYO) and the other 20 made up the healthy group (control; CTR). We measured concentrations of hs-cTnI, aspartate aminotransferase (AST), creatine kinase (CK) and l-lactate in queens from both groups. Additionally, we measured cytokine concentrations in all queens. Results: The hs-cTnI concentration in the PYO group (26.95 ± 5.08 ng/L) was significantly higher than that of the CTR group (7.00 ± 0.82 ng/L) (p < 0.000). Furthermore, the PYO group had a higher CK concentration (344.50 ± 39.63 U/L) than the CTR group (191.00 ± 15.44 U/L) (p = 0.002). The PYO group also demonstrated higher concentrations of TNF-α (9.77 ± 0.81 ng/mL), IFN-γ (25.37 ± 2.09 ng/mL), IL-2 (4.37 ± 0.39 ng/mL), IL-4 (245.64 ± 15.83 pg/mL), IL-5 (63.13 ± 1.65 pg/mL) and IL-10 (123.58 ± 4.30 ng/mL) compared to the CTR group (p < 0.000). Conclusions: Overall, it is suggested that changes in cytokine concentrations increase in queens with pyometra, potentially causing harm to the heart muscle. It is crucial to consider that the heart muscle may also be affected in queens with pyometra during the treatment process.
Prevalence, molecular identification and genotyping of the crayfish plague pathogen, Aphanomyces astaci in major narrow-clawed crayfish (Pontastacus leptodactylus Eschscholtz, 1823) populations from Türkiye
(2024) Akhan, S.; Çağatay, İ.T.; Berber, S.; Taştan, B.; Taştan, Y.; Dalar, T.
Introduction: Crayfish plague is considered the most important crayfish disease globally. It is caused by the fungus-like agent, Aphanomyces astaci. This study aimed to identify and determine the prevalence of A. astaci using PCR in narrow-clawed crayfish (Pontastacus leptodactylus) populations from across Türkiye. Material and methods: A PCR was carried out with primers specific to the internal transcribed spacer region of the A. astaci pathogen on both telson and abdominal cuticle tissues from crayfish individuals from 41 different locations. Results: Aphanomyces astaci was detected in the crayfish from 34 of the locations. Molecular diagnosis showed the prevalence rates of A. astaci to be between 0% and 68.2%. For 7 of the 34 locations, the strain of A. astaci was determined. Microsatellite analysis of tissue from individuals with positive PCR results revealed the A. astaci genotypes in seven populations. Genotype B was found to be the predominant genotype responsible for crayfish plague in Turkish crayfish populations. The Psl genotype (genotype B) was determined in six of the populations, and the As genotype (genotype A) was detected in only one. Conclusion: Crayfish plague poses a significant threat to crayfish populations, necessitating the development of rapid, highly sensitive diagnostic methods. An understanding of the sensitivity of the PCR detection method and of the prevalence and genotyping of A. astaci in Turkish crayfish populations has been gained from this study.
Albumin-based nanocarriers loaded with novel Zn(II)-thiosemicarbazone compounds chart a new path for precision breast cancer therapy
(2025) Kalindemirtaş, F.D.; Özerkan, D.; Kariper, İ.A.; Erdemir C.G.; Ülküseven, B.; Kuruca, S.E.
This study explores the therapeutic potential of albumin-bound Zn(II)-thiosemicarbazone compounds (Alb-ZnTcA, Alb-ZnTcB) against breast cancer cells. Previous research indicates that these compounds hinder cancer cell proliferation by blocking DNA synthesis, promoting oxidative stress to induce apoptosis, and disrupting the cell cycle to inhibit cellular division. This study focuses on the loading and characterization of these potentially chemically unstable compounds on bovine serum albumin-based nanocarriers. Accordingly, unlike previous studies using albumin nanoparticles, in this study, ultraviolet light was used to precisely bind the therapeutic agent to albumin during the integration of thiosemicarbazones, achieving controlled nanoparticle size to control nanoparticle size. The mean diameter of Alb-ZnTcA nanoparticles was 32 nm, while Alb-ZnTcB exhibited an average diameter of 43 nm. Notably, Alb-ZnTcA displayed the highest cytotoxicity toward breast cancer cells, suggesting an optimal size for cellular uptake. Additionally, albumin-bound compounds showed enhanced cytotoxicity at lower concentrations, potentially minimizing adverse side effects. Apoptosis analysis indicated that both Alb-ZnTcA and Alb-ZnTcB induce cell death predominantly through apoptosis, effectively preventing the uncontrolled proliferation of cancer cells. These findings demonstrate the potential of Zn(II)-thiosemicarbazone compounds loaded on albumin-based nanocarriers for breast cancer treatment. The increased potency of Alb-ZnTcA and Alb-ZnTcB compared to free compounds, along with their ability to activate apoptotic signaling pathways in MCF-7 breast cancer cells, highlights a promising approach for future cancer therapies. This study suggests that albumin-bound Zn(II)-thiosemicarbazone compounds could offer a targeted and effective strategy in breast cancer treatment, leveraging the advantages of nanocarrier-based delivery systems.
The Association of Sarcopenia and Body Composition With Benign Positional Paroxysmal Vertigo in Older Adults
(2025) Demircan, S.K.; Öner, F.
Objectives: One-third of older adults suffer from dizziness and vertigo. Benign positional paroxysmal vertigo (BPPV), which occurs due to otoconia moving into the semicircular canal, is the most common vestibular disorder. We evaluated the connection between BPPV and geriatric symptoms. Methods: A comprehensive geriatric evaluation included daily living activities, Mini-Mental State Examination (MMSE), and Geriatric Depression Scale tests for all patients. Patients' nutritional status was assessed using the Mini-Nutritional Assessment. Body weight and fat free mass were determined from the bioimpedance analyzer. Hand grip strength was measured using an electronic hand dynamometer to determine muscle strength. Sarcopenia was assessed using the European Working Group on Sarcopenia in Older People-2 recommendations. The patients were divided into three groups as BPPV-positive, BPPV-negative, and healthy (control). Results: Obesity, low muscle mass, dynapenia, gait speed, low gait speed, and history of falling statistically significantly differed between the three groups, but age, gender, smoking, alcohol consumption, body mass index, MMSE, depression, number of falls, and comorbid diseases did not differ. The BPPV-positive group had greater rates of obesity, low muscle mass, dynapenia, and sarcopenia than the control group (p = 0.008, 0.007, 0.01, 0.03). In the unadjusted univariate analysis, low muscle mass, sarcopenia, and obesity were risk factors for "BPPV" (Odds Ratio [OR]: 3.43, Confidence Interval [Cl]: 1.25-9.37, p = 0.016; OR: 3.47, Cl: 1.32-9.13, p = 0.011; OR: 2.71, Cl: 1.09-6.70, p = 0.031). Conclusions: Obesity, sarcopenia, and low muscle mass are risk factors for BPPV, and we urge the older population to adopt healthy diet and exercise regimens to reduce BPPV-related falls.
Effects of urban sprawl due to migration on spatiotemporal land use-land cover change: a case study of Bartın in Türkiye
(2025) Şen, G.
Rapid urban growth is a subject of worldwide interest due to environmental problems. Population growth, especially migration from rural to urban areas, leads to land use and land cover (LULCC) changes in urban centres. Therefore, LULCC and urban growth analyses are among the studies that will help decision-makers achieve better sustainable management and planning. The objective of this study was to ascertain the impact of urbanization, which resulted from migration, on the alterations in LULCC, with a particular focus on the changes in forest areas surrounding the Bartın city centre between 2000 and 2020. Spatial databases for two periods were used to determine changes in urban growth. The spatial and temporal LULCC patterns of land use were quantified by interpreting spatial data. Remote sensing (RS) and geographical information systems (GIS) have been used for data collection, analysis, and presentation. The LULCC was assessed under nine classes using optical remote sensing methods on stand-type maps created from aerial photos. To determine how urban growth affects LULCC, land use status and transition matrices were created for each of the five sprawl zones around Bartın city. The annual change in forest areas is determined by the "annual forest rate". The results indicate that the urbanization of Bartın city from 2000 to 2020 increased by approximately 19% (2510645.82 m). However, this did not harm the forests; cover increased by 10.32% (174729.65 m²) over the same period. The process of urbanization was particularly evident in open areas and agricultural zones. During this period, there was a 37% reduction in agricultural areas (2943229.85 m²) and a 59% reduction in open areas (1265457.76 m²). The sprawl of Bartın city can be attributed to changes in its demographic structure, which mainly includes the migration of the rural population to urban areas and the emergence of new job opportunities. Factors such as challenging urban living conditions, insecure environments because of the increase in temporary foreign asylum seekers, and retirees returning to their hometowns are believed to have contributed to this population growth.
Morphological Features of the Pecten Oculi in the Common Kestrel (Falco tinnunculus)
(2025) Kilinç, B.; Toprak, B.; Yüksel, S.; Kandir, E.H.; Koçak, G.
This study aimed to investigate the macroscopic, light microscopic (LM) and scanning electron microscopic (SEM) characteristics of the pecten oculi in common kestrels (Falco tinnunculus). A total of six eyeballs from three common kestrels were used as the study material. The examination revealed that the bulbus oculi was spherical in shape and its diameter exceeded the axial-global length. In the equatorial section of the eyeball, the pecten oculi were observed to be situated on the retina layer where the optic nerve enters the eyeball, showing a posterior-to-anterior distribution. The pecten oculi appeared dark brown, with a convoluted structure, comprising approximately 15-17 vascular folds. Scanning electron microscopic examination revealed dense capillary vascular folds on the surface of the pecten oculi. Within these capillary vessels, a few hyalocytes were noted, and melanocytes were found at the edges of the folds. Light microscopic examination detected numerous large, medium and small diameter vessels within the folds of the pecten oculi, especially at the periphery of the folds and the apical parts of the pecten oculi. The findings of this study were compared with the morphological characteristics of the pecten oculi in other bird species, revealing both similarities and differences.
Monitoring the operational changes in surface reflectances after logging, based on popular indices over Sentinel-2, Landsat-8, and ASTER imageries
(2025) Genç, Ç.Ö.; Altunel, A.O.
Revealing the status of forests is important for sustainable forest management. The basis of the concept lies in meeting the needs of future generations and today's generations in the management of forests. The use of remote-sensing (RS) technologies and geographic information systems (GIS) techniques in revealing the current forest structure and in long-term planning of forest areas with multipurpose planning techniques is increasing day by day. Significant technological advances are in allowing programmers to modernize how they manage data. Sentinel-2, which is a relatively new addition to Earth observing satellites, is a new-generation satellite that has enabled classification and monitoring of land cover change with high precision at ease. Visible R, G, B, and near-infrared (NIR) bands have offered exceptional 10-m spatial reasolution, making them suitable for vegetation monitoring along with the additional 20-m bands to spare especially in chlorophyll content analyses. On the contrary, Landsat-8 and ASTER which have been longer lasting in Earth observation were rougher results especially in forestry studies. In this study, Landsat-8 and ASTER satellite images were compared against the Sentinel-2 images as a reference in conjunction with GIS techniques to monitor and assess the impact of various logging procedures, including selective logging and regeneration silviculture. The investigation employed a range of plant vegetation indices, including NDVI, GNDVI, and SAVI, to evaluate the efficacy of image resolution in detecting forest cover changes in the Kastamonu region, where the timber production is the hightest in Turkey. For selective and regeneration activities, satellite images were taken pre-harvesting and immediately post-harvesting, and index maps were produced. NDVI, GNDVI, and SAVI indices were the most accurate indicators of green vegetation change in the Sentinel-2A imagery. Similarly, for the Landsat-8 imagery, the SAVI, NDVI, and GNDVI indices were found to be satisfactory indicators. As for ASTER imagery, the success sequance was like SAVI, GNDVI, and NDVI. Based on the findings of this study, it has been noted that the ASTER imagery closeness to Sentinel-2A was more remarkable in detecting changes in green vegetation in forested areas. The data derived from ASTER imageries demonstrated superior efficacy compared to Landsat-8 in generating forest cover maps, owing to their proximity to those produced by Sentinel-2. The findings also indicated that ASTER imagery, with suitable spatial and spectral resolution, could still be utilized as efficienly as Landsats to generate forest cover density maps and monitor long-term forest conservation practices, particularly in professionally managed forests. Thus, this methodology demonstrated the capacity for efficient worldwide forest management.
Climate change impact on blood haemogram in the horse: a three-year preliminary study
(2024) Deniz, Ö.; Aragona, F.; Murphy, B.A; Tümer, K.Ç.; Bozacı, S.; Fazio, F.
Introduction: The global climatic changes pose a substantial threat to the well-being and productivity of both humans and animals. Methods: This study examined the impact of climate changes during different seasons over a 3-year monitoring period (2021-2023) on various blood parameters including, white blood cells (WBC), neutrophils, basophils, eosinophils, lymphocytes, and monocytes, hematocrit (HCT), hemoglobin (HGB), red blood cells (RBC), platelets (PLT), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH). The study focused on 25 Thoroughbred mares located in Kastamonu-Türkiye. Thermal and hygrometric parameters, including ambient temperature, relative humidity, and ventilation, were collected. Subsequently, Temperature-Humidity index (THI) was computed. Blood samples were collected on the first day of every month from January 2021 to December 2023 and used for a complete blood count analysis. Between 2021 and 2023, changes in environmental indicators were correlated to changes in hematological parameters. Results: Two-way for repeated measures ANOVA revealed a significant seasonal fluctuation (<0.0001) in ambient temperature, relative humidity, and THI. There was a reduction in RBC (<0.01), and MCH (<0.01) every year, HGB (<0.0001) in summer 2021, 2022 and in summer and autumn 2023. HCT (<0.0001), MCV (<0.01), showed decreasing values in autumn 2022 and 2023. MCHC values showed increasing values in July and August 2021, 2022 and in June 2023. WBC levels increased throughout the spring periods of 2021 and 2022. In April 2021, there were elevated levels of lymphocytes and monocytes (<0.0001) respectively. Discussion: These findings could be helpful to promote the monitoring of physiological status both for the assessment of welfare status and for diagnostic purposes for the evaluation of possible disease outbreaks due to climate change in veterinary medicine.
Could Serum Collectin-11, surfactant protein-A and D be suitable biomarkers for assessing pulmonary damage caused by Rhodococcus equi infection in foals on large farms?
(2024) Ekinci, G.; Deniz, O.; Tüfekci, E.; Timur, M. C.; Onmaz, A. C.; Günes, V.; Citil, M.; Keles, I.; Hoven, R. V. D.
Rhodococcus equi (R. equi) is a primary cause of pyogranulomatous pneumonia of foals between three weeks and five months of age. Early diagnosis of rhodococcal pneumonia has always been considered a preferable approach as it can lead to more successful treatment and better outcomes. Horse stud farms where the disease is common, neonatal foals are subjected to tests such as complete blood count and fibrinogen analysis at certain intervals. However, new biomarkers are needed in addition to blood count and fibrinogen measurement in this field for early diagnosis of diseases. Based on this need, in this study, the diagnostic importance of CL-11, SP-A, SP-D was investigated for the early diagnosis of pneumonia in foals naturally infected with R. equi. In a case-control design, fourteen 1-5 month-old foals with proven R. equi pyogranulomatous pneumonia and 10 healthy 1-5 month-old control foals were enrolled in this study. The median white blood cell count (WBC) and fibrinogen concentration in the case group were significantly higher than in the control group. The median CL-11, SP-A, and SP-D concentrations in the case group were also significantly higher than in the control group. However, there were overlaps in concentrations between groups for SP-A and SP-D. There was less overlap between the groups for the CL-11 concentration. The CL-11 assay was sufficiently accurate but over-diagnosed R. equi infection. The correlation plot between fibrinogen and CL-11 concentrations shows that this problem may be solved if CL-11 is used as the first biomarker and fibrinogen as the second check for those foals with CL-11 serum levels >0.8 and ≤1.5 ng/mL. As a result, in this study, it is recommended to use CL-11 together with fibrinogen to obtain more accurate results in diagnosing pyogranulomatous pneumonia caused by R. equi in foals on large horse stud farms.
The effects of a home visit-supported nursing education program on certain parameters in older adults living alone at home
(2024) Arabacı, Z.; Kaçan, H.
Background: The rapid aging of the world population is emerging as a global public health issue, and the likelihood of living alone increases with age. Aim: This study aims to develop healthy lifestyle behaviors, reduce loneliness levels, and increase social support capacity among older adults living alone at home. Research model: The present study was designed as a quasi-experimental study. Method: This study included 49 older adults aged 65 and above who agreed to participate, with 26 in the experimental group and 23 in the control group. The data collection tools used were the Introductory Information Form, the healthy lifestyle behavior scale II, the UCLA loneliness scale Short Form-6, and the multidimensional scale of Perceived Social Support. The experimental group received individualized nursing education programs through weekly 60-min home visits for 8 weeks. The study followed the CONSORT flow diagram. Results: The results indicate a statistically significant difference between the pretest and posttest scores of the experimental group in the "Friend" dimension of the Perceived Social Support Scale. However, no difference was found in the healthy lifestyle behavior scores and the loneliness scale scores. Conclusion: It is difficult to achieve significant improvements in short periods among older adult groups. Substantial changes may not have occurred because this study included pretest and posttest measures over an 8-week period. Therefore, future studies should consider longer durations and include follow-up processes to observe more significant effects. Implications for nursing practice: It is anticipated that there will be a successful aging process in studies supported by long-term home visits by nurses.
Diverticulitis with Colovenous Fistula and Pylephlebitis: Imaging Findings of a Rare Case
(2024) Koyun, M.; Ece, B.
Opportunities and Challenges of Chatbots in Ophthalmology: A Narrative Review
(2024) Sabaner, M.C.; Anguita, R.; Antaki, F.; Balas, M.; Boberg-Ans, L.C.; Ferro Desideri, L.; Grauslund, J.; Hansen, M.S.; Klefter, O.N.; Potapenko, I.; Rasmussen, M.L.R.; Subhi, Y.
Artificial intelligence (AI) is becoming increasingly influential in ophthalmology, particularly through advancements in machine learning, deep learning, robotics, neural networks, and natural language processing (NLP). Among these, NLP-based chatbots are the most readily accessible and are driven by AI-based large language models (LLMs). These chatbots have facilitated new research avenues and have gained traction in both clinical and surgical applications in ophthalmology. They are also increasingly being utilized in studies on ophthalmology-related exams, particularly those containing multiple-choice questions (MCQs). This narrative review evaluates both the opportunities and the challenges of integrating chatbots into ophthalmology research, with separate assessments of studies involving open- and close-ended questions. While chatbots have demonstrated sufficient accuracy in handling MCQ-based studies, supporting their use in education, additional exam security measures are necessary. The research on open-ended question responses suggests that AI-based LLM chatbots could be applied across nearly all areas of ophthalmology. They have shown promise for addressing patient inquiries, offering medical advice, patient education, supporting triage, facilitating diagnosis and differential diagnosis, and aiding in surgical planning. However, the ethical implications, confidentiality concerns, physician liability, and issues surrounding patient privacy remain pressing challenges. Although AI has demonstrated significant promise in clinical patient care, it is currently most effective as a supportive tool rather than as a replacement for human physicians.
Developing the developmental origins of health and disease (DOHaD) awareness scale to assess an education module for improving dietary behavior among college students
(2024) İmre, K.E.; Akyol, A.
Objective: This study aimed to develop and validate the Developmental Origins of Health and Disease (DOHaD) awareness scale and examine whether having a DOHaD education module may affect dietary behavior in college students. Background: Some studies conducted within the scope of the DOHaD hypothesis show associations between early-life environmental factors, especially maternal health and nutritional status, with the next generation's health and disease status. Despite the increase in elucidating of the underpinning mechanisms of early life determinants and chronic disease risk, there is limited knowledge on how public perceive and understand DOHaD concepts. Methods: The study consisted of three phases: identification of DOHaD awareness scale components, development and validation, interrater reliability of the scale, and a confirmatory study. Two-hundred college students to confirm face validity of the scale, one-hundred for reproducibility and one-hundred for the confirmatory study. Confirmatory study included a pre-intervention period in which baseline parameters (such as anthropometric measurements, HEI-2015 (Healthy Eating Index-2015), physical activity levels, and DOHaD awareness scale scores) were measured at two different time points, and an educational module on DOHaD concepts was provided to the participants. The study was reported in accordance with the Guidelines for Reporting Reliability and Agreement Studies (GRRAS). Results: Content validity ratio, exploratory factor analysis, and Cronbach's alpha values indicated that a reliable and valid instrument to assess the awareness of DOHaD concepts in college students was developed. Despite the lack of a control group, DOHaD concept education was associated with improved anthropometric measurements, healthier eating parameters, increased physical activity levels, and a better understanding of DOHaD concepts. Conclusions: Translating DOHaD concepts into healthier behaviors can support improvements in lifestyle, and the use of the DOHaD awareness scale may serve as a valuable tool to encourage healthy behaviors among college students.
Exploring the anti‑inflammatory activity of boron compounds through the miR‑21/PTEN/AKT pathway in cecal ligation and puncture‑induced sepsis
(2025) Sevim, Ç.; Ozkaraca, M.; Kara, M.; Taghizadehghalehjoughi, A.; Genç, S.; Yeni, Y.; Mendil, A.S.; Spanakis, M.; Ozcagli, E.; Kuzmin, S.V; Spandidos, D.A; Tsatsakis, A.
The present study investigated the impact of boric acid (BA) and borax (BX) on markers of inflammation and modifications in miR‑21/PTEN/AKT pathway genes in the liver and kidney tissues of Sprague Dawley male rats with sepsis induced by cecal ligation and puncture (CLP). A total of 60 male Sprague Dawley rats were randomly divided into 6 groups, each containing 10 animals as follows: Control, CLP (where the model was created), 20 mg/kg BX (CLP + BX1), 40 mg/kg BX (CLP + BX2), 20 mg/kg BA (CLP + BA1) and 40 mg/kg BA (CLP + BA2). Liver and kidney tissues were analyzed for histopathological changes, immunopositivity for tumor necrosis factor‑α, interleukin (IL)‑6 and IL‑10, and gene expression of microRNA‑21 (miR‑21), phosphatase and tensin homolog (PTEN) and AKT. Gene expression analysis in the liver tissues revealed a significant decrease in miR‑21, and a marked but not significant decrease in PTEN levels in the CLP group, while AKT expression was significantly increased in the CLP group, and was significantly decreased in CLP + BA1 group compared with in the CLP group. In the kidney tissues, miR‑21 levels were significantly decreased in the CLP group, but the CLP + BA2 group showed a significant increase compared with in the CLP group. These results suggest the potential therapeutic benefits of low‑dose BA and BX in ameliorating sepsis‑induced tissue damage, emphasizing the need for further exploration of their mechanisms of action.
Could "Sub-RPE illumination" be a prognostic marker? A prospective dry AMD study
(2024) Yozgat, Z.; Durmus Ece, B.S.; Isik, M.U.; Ilguy, S.; Sabaner, M.C.
Purpose: To evaluate the natural history of dry age-related macular degeneration (AMD) through advanced retinal pigment epithelium (RPE) analysis and sub-RPE illumination (SRI) data and to elucidate their correlation with disease progression. Methods: A total of 82 patients with dry AMD were included in this longitudinal study. Spectral-domain optical coherence tomography (SD-OCT) was utilized to evaluate central macular thickness (CMT), central retinal thickness (CRT), foveal outer nuclear layer (ONL) thickness, and ellipsoid zone (EZ) integrity. Advanced retinal pigment epithelium (RPE) analysis software was used to obtain area and volume data at 3mm and 5mm circles, as well as sub-RPE illumination (SRI) assessments within 5mm circles. Results: After exclusions, the final cohort consisted of 54 eyes of 54 patients (29 female), with a mean age of 74.72±8.38years and a mean follow-up period of 13.92±1.7months. Both area (mm2) and volume (mm3) within the 3mm and 5mm circles were significantly increased at the final visit (all P<0.001). From the SRI data, the area within the 5mm circle (mm2) was also identified as significantly higher at the final visit (P<0.001). CMT, CRT, and ONL thickness decreased significantly by the final visit (P=0.014, P<0.001, and P<0.001, respectively). Baseline and final LogMAR visual acuity values showed a significant inverse correlation with the distance of SRI from the fovea (P=0.03, r=-0.347 and P=0.04, r=-0.382, respectively). Baseline SRI values were higher in patients with EZ disruption at onset [0.8 (0.1-1.19) vs. 0.0 (0-0.1), P=0.002]. Furthermore, patients with initial EZ disruption had significantly higher final SRI values than those without [1.1 (0.3-2.1) vs. 0.1 (0.1-0.2), P=0.038]. A significant positive correlation was found between the initial SRI area and final LogMAR visual acuity (P<0.001, r=0.645). Conclusion: Initial SRI area may predict the risk of vision loss over a 12-month follow-up period and could serve as a prognostic marker for progression of dry AMD.

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