Pubmed:
Medicinal evaluation and molecular docking study of osajin as an anti-inflammatory, antioxidant, and antiapoptotic agent against sepsis-associated acute kidney injury in rats

dc.contributor.authorAlhilal, M.
dc.contributor.authorErol, H.S.
dc.contributor.authorYildirim, S.
dc.contributor.authorCakir, A.
dc.contributor.authorKoc, M.
dc.contributor.authorAlhilal, S.
dc.contributor.authorDereli, E.
dc.contributor.authorAlkanoglu, O.
dc.contributor.authorAy, V.
dc.contributor.authorCan, I.
dc.contributor.authorHalici, M.B.
dc.date.accessioned2024-07-23T08:32:43Z
dc.date.available2024-07-23T08:32:43Z
dc.date.issued2024
dc.description.abstractDespite efforts to find effective drugs for sepsis-associated acute kidney injury (SA-AKI), mortality rates in patients with SA-AKI have not decreased. Our study evaluated the protective effects of isoflavone osajin (OSJ) on SA-AKI in rats by targeting inflammation, oxidative stress, and apoptosis, which represent the cornerstones in the pathophysiological mechanism of SA-AKI. Polymicrobial sepsis was induced in rats the cecal ligation and puncture (CLP) technique. Markers of oxidative stress were evaluated in kidney tissues using biochemical methods. The expression of interleukin-33 (IL-33), 8-hydroxydeoxyguanosine (8-OHdG), caspase-3, and kidney injury molecule-1 (KIM-1) was evaluated as indicators of inflammation, DNA damage, apoptosis, and SA-AKI respectively in the kidney tissues using immunohistochemical and immunofluorescent detection methods. The CLP technique significantly ( < 0.001) increased lipid peroxidation (LPO) levels and significantly ( < 0.001) decreased the activities of superoxide dismutase and catalase in kidney tissues. In the renal tissues, strong expression of IL-33, 8-OHdG, caspase-3, and KIM-1 was observed with severe degeneration and necrosis in the tubular epithelium and intense interstitial nephritis. In contrast, the administration of OSJ significantly ( < 0.001) reduced the level of LPO, markedly improved biomarkers of antioxidant status, decreased the levels of serum creatinine and urea, lowered the expression of IL-33, 8-OHdG, caspase-3, and KIM-1 and alleviated changes in renal histopathology. A promising binding score was found a molecular docking investigation of the OSJ-binding mode with mouse IL-33 (PDB Code: 5VI4). Therefore, OSJ protects against SA-AKI by suppressing the IL-33/LPO/8-OHdG/caspase-3 pathway and improving the antioxidant system.
dc.identifier.doi10.1080/0886022X.2024.2379008
dc.identifier.pubmed39034431
dc.identifier.urihttps://hdl.handle.net/20.500.12597/33446
dc.language.isoen
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectApoptosis
dc.subjectSA-AKI
dc.subjectinflammation
dc.subjectosajin
dc.subjectoxidative stress
dc.titleMedicinal evaluation and molecular docking study of osajin as an anti-inflammatory, antioxidant, and antiapoptotic agent against sepsis-associated acute kidney injury in rats
dc.typeArticle
dspace.entity.typePubmed

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