Pubmed: The Novel 5-Fluorouracil Loaded Ruthenium-based Nanocarriers Enhanced Anticancer and Apoptotic Efficiency while Reducing Multidrug Resistance in Colorectal Cancer Cells.
dc.contributor.author | Danişman-Kalindemirtaş, Ferdane | |
dc.contributor.author | Özerkan, Dilşad | |
dc.contributor.author | Kariper, İshak Afşin | |
dc.contributor.author | Bulut, Huri | |
dc.date.accessioned | 2023-04-06T21:33:15Z | |
dc.date.available | 2023-04-06T21:33:15Z | |
dc.date.issued | 2023-02-22T00:00:00Z | |
dc.description.abstract | Recently, nanocarriers have been made to eliminate the disadvantages of chemotherapeutic agents by nanocarriers. Nanocarriers show their efficacy through their targeted and controlled release. In this study, 5-fluorouracil (5FU) was loaded into ruthenium (Ru)-based nanocarrier (5FU-RuNPs) for the first time to eliminate the disadvantages of 5FU, and its cytotoxic and apoptotic effects on HCT116 colorectal cancer cells were compared with free 5FU. 5FU-RuNPs with a size of approximately 100 nm showed a 2.61-fold higher cytotoxic effect compared to free 5FU. Apoptotic cells were detected by Hoechst/propidium iodide double staining, and the expression levels of BAX/Bcl-2 and p53 proteins, in which apoptosis occurred intrinsically, were revealed. In addition, 5FU-RuNPs was also found to reduce multidrug resistance (MDR) according to BCRP/ABCG2 gene expression levels. When all the results were evaluated, the fact that Ru-based nanocarriers alone did not cause cytotoxicity proved that they were ideal nanocarriers. Moreover, 5FU-RuNPs did not show any significant effect on the cell viability of normal human epithelial cell lines BEAS-2B. Consequently, the 5FU-RuNPs synthesized for the first time may be ideal candidates for cancer treatment because they can minimize the potential drawbacks of free 5FU. | |
dc.identifier.doi | 10.1007/s10895-023-03180-9 | |
dc.identifier.issn | 1573-4994 | |
dc.identifier.pubmed | 36811696 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12597/3239 | |
dc.language.iso | en | |
dc.relation.ispartof | Journal of fluorescence | |
dc.subject | 5FU | |
dc.subject | Apoptosis | |
dc.subject | Colorectal cancer | |
dc.subject | Cytotoxicity | |
dc.subject | Ruthenium-based nanoparticles | |
dc.title | The Novel 5-Fluorouracil Loaded Ruthenium-based Nanocarriers Enhanced Anticancer and Apoptotic Efficiency while Reducing Multidrug Resistance in Colorectal Cancer Cells. | |
dc.type | Journal Article | |
dspace.entity.type | Pubmed | |
relation.isPublicationOfPubmed | 470e91d5-7c64-433b-aad9-041641d1b9e0 | |
relation.isPublicationOfPubmed.latestForDiscovery | 470e91d5-7c64-433b-aad9-041641d1b9e0 |