Lanostane-Type Triterpenes and Abietane-Type Diterpene from the Sclerotia of Chaga Medicinal Mushroom, Inonotus obliquus (Agaricomycetes), and Their Biological Activities.

Abstract

Three new lanostane-type triterpenes (compounds 1-3), 1 new abietane-type diterpene (compound 4), and 10 known compounds (5-14) were isolated from sclerotia of Inonotus obliquus. Their structures were elucidated through a combination of spectrometric techniques, including infrared, 1-dimensional, and 2-dimensional nuclear magnetic resonance and high-resolution electrospray mass spectrometry. In in vitro assays, compounds 2 and 4-12 showed hepatoprotective effects against D-galactosamine-induced damage in WB-F344 cells, with inhibitory effects from 35.4% to 83.8%. Compounds 3, 13, and 14 exhibited selective cytotoxicity against Bel-7402, A-549, or KB cell lines. Compounds 13 and 14 showed inhibitory effects against protein tyrosine kinases, with half-maximal inhibitory concentrations of 23.8 and 7.4 μmol/L, respectively.