High hydrostatic pressure processing: A method having high success potential in pollen protein extraction

Abstract

Even a single peptide that is present in the pollen wall and cytoplasm could cause pollen allergy. To produce skin-prick test kits, the first step is the extraction of these molecules. In this study, Cedrus atlantica pollens were subjected to 220 and 330MPa for 10 and 30min in order to extract these molecules. After high hydrostatic pressure processing (HHPP), the total amounts of proteins (TAPs) are measured and compared with the results of the conventional extraction method (CEM). As a result, the TAPs extracted by HHPP is 18.0210 μg/mL at 220MPa for 10min, 22.5770 μg/mL at 220MPa for 30min, 23.3810 μg/mL at 330MPa for 10min and 25.9270 μg/mL at 330MPa for 30min, while this is 1.9460 μg/mL in 24h by the CEM. In addition to these results, visual pollen deformation and eruption, pollen wall and surface damage have also been observed. © 2012 Taylor & Francis.