Publication: Lipidomic markers of sperm cryotolerance in cattle.
dc.contributor.author | Evans, Holly C, Dinh, Thu T N, Ugur, Muhammet Rasit, Hitit, Mustafa, Sajeev, Dishnu, Kaya, Abdullah, Topper, Einko, Nicodemus, Molly C, Smith, Gary D, Memili, Erdogan | |
dc.contributor.author | Evans, HC, Dinh, TTN, Ugur, MR, Hitit, M, Sajeev, D, Kaya, A, Topper, E, Nicodemus, MC, Smith, GD, Memili, E | |
dc.date.accessioned | 2023-05-09T16:17:37Z | |
dc.date.available | 2023-05-09T16:17:37Z | |
dc.date.issued | 2020-11-19T00:00:00Z | |
dc.date.issued | 2020.01.01 | |
dc.description.abstract | The objective of the current study was to determine the fatty acid composition of sperm from Holstein bulls with different freezability (Good and Poor; n = 12). Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform-methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography-mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (P < 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18 µg) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9 µg; P < 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P ≤ 0.011). These results are significant because they reveal key understandings of fatty acid composition of sperm membrane and lay a foundation for the manipulation of membrane integrity, fluidity, and stability to advance the assisted reproductive technologies. | |
dc.identifier.doi | 10.1038/s41598-020-77089-9 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.pubmed | 33214639 | |
dc.identifier.scopus | 2-s2.0-85096308777 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12597/13111 | |
dc.identifier.volume | 10 | |
dc.identifier.wos | WOS:000594637300006 | |
dc.relation.ispartof | Scientific Reports | |
dc.relation.ispartof | SCIENTIFIC REPORTS | |
dc.rights | true | |
dc.title | Lipidomic markers of sperm cryotolerance in cattle. | |
dc.title | Lipidomic markers of sperm cryotolerance in cattle | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
oaire.citation.issue | 1 | |
oaire.citation.volume | 10 | |
relation.isPubmedOfPublication | 838108b3-02a5-4def-85b0-c80784cfea29 | |
relation.isPubmedOfPublication.latestForDiscovery | 838108b3-02a5-4def-85b0-c80784cfea29 | |
relation.isScopusOfPublication | 7ef14e81-f96d-4263-9ce2-c86163bfe59a | |
relation.isScopusOfPublication.latestForDiscovery | 7ef14e81-f96d-4263-9ce2-c86163bfe59a | |
relation.isWosOfPublication | a52251f6-e1bd-4a0f-a8a1-b52a5fc3830f | |
relation.isWosOfPublication.latestForDiscovery | a52251f6-e1bd-4a0f-a8a1-b52a5fc3830f |