Phytotoxicity, Toxicity on Brine Shrimp and Insecticidal Effect of Aytac & Anderb. Growing in Turkey.

Abstract

The aim of this study was to investigate the probable toxicity on brine shrimp, phytotoxicity, and insecticidal activity of Aytac & Anderb.

Objectives: The aim of this study was to investigate the probable toxicity on brine shrimp, phytotoxicity, and insecticidal activity of Chrysophthalmumgueneri Aytac & Anderb.Materials and Methods: The MeOH (80%) extract obtained from the whole plant of C. gueneri was fractionated through subsequent solventextractions in increasing polarity with n-hexane, chloroform, and n-butanol. The MeOH (80%) extract and all fractions of C. gueneri were evaluatedfor their biologic activities using in vitro screening bioassays such as brine shrimp lethality test and phytotoxicity against Lemna minor, as well asinsecticidal activity against Rhyzopertha dominica and Tribolium castaneum.Results: The findings showed that the n-hexane and chloroform fractions of the plant had significant phytotoxic activities with 100% growthinhibition (GI) at 1000 μg/mL against L. minor. Moreover, the MeOH (80%) extract (53% GI) and n-butanol fraction (46.6% GI) of the plant hadmoderate phytotoxic activities at 1000 μg/mL. Otherwise, no samples had toxicity on the brine shrimps. In addition, the remaining water fractionhad low insecticidal activity with 20% mortality against T. castaneum.Conclusion: Our results show that the n-hexane and chloroform fractions of C. gueneri had potential phytotoxic effects.