Studies on Antimicrobial, Antibiofilm, Efflux Pump Inhibiting, and ADMET Properties of Newly Synthesized 1,3,4-Thiadiazole Derivatives**

Abstract

The novel compounds bearing 1,3,4-thiadiazole moiety were obtained and their structures were identified using FT-IR, NMR methods, elemental, and mass analysis. UV-Vis and fluorescence spectra were obtained in six different polarity solvents. In addition, antimicrobial, antibiofilm, and the efflux pump inhibiting activities of the new compounds on K. pneumoniae, methicillin-resistant S. aureus (MRSA), E. coli, and C. albicans were investigated. Furthermore, druglikeness and ADMET properties of the compounds were determined. As a result, it was observed that some of the derivatives presented antimicrobial activity against K. pneumoniae, S. aureus, and C. albicans. But the MBC/MFC test showed that all the activities were bacteriostatic. In addition, these derivatives have the potential of using both inhibiting and activating biofilm formation depending on the concentration. 1,3,4-thiadiazole derivatives also presented good efflux pump inhibition in K. pneumoniae and E. coli. ADMET tests showed that 1,3,4-thiadiazole derivatives have the potential of being used as therapeutic compounds.