The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis

Abstract

Aim: Sepsis is a fatal serious clinical disorder. The oxidative stressin sepsis plays an important role in the inflammation. Therefore, theeffects of oleuropein (OLE) and ceftriaxone (CEFT) on sepsis wereinvestigated in this study.Materials and Methods: Thirty male rats were divided into fiveequal groups: Sham, Sepsis, CEFT, OLE-150 and OLE-300, The150 mg/kg of ceftriaxone (i.p.), and 150 and 300 mg/kg OLE (p.o.)were administered to the treatment groups fifteen minutes beforethe experiment. After 24 hours, blood samples, lungs and hearts tissues were taken. The creatine kinase (CK) and lactate dehydrogenase (LDH) activities in serum, lipid peroxidation (LPO), glutathione(GSH), superoxide dismutase (SOD) and catalase (CAT) levels wereall determined. In addition, the expressions of the interleukin (IL)-8in lung and tumor necrosis factor (TNF)-α in heart were pathologically and immunohistochemically were determined.Results: Sepsis caused significant increases in CK, LDH, LPO, GSHand heart CAT activity and caused inhibition of SOD and lung CATactivity (p<0.001). The OLE significantly decreased the LPO and GSHlevels (p<0.001) and increased the SOD in activity of both tissuesand CAT activity in lung tissue (p<0.001). Moreover, CEFT significantly decreased the LPO level (p<0.01) and increased tissue SODand CAT activities in lung tissue (p<0.001). CK and LDH activities inserum showed marked decrease in OLE and CEFT groups (p<0.001).Conclusion: Antioxidant and a notable anti-inflammatory effect ofOLE by reducing IL-8 and TNF-α levels have been stated. Thus, astrong antioxidant, such as OLE, would be beneficial for the treatment of sepsis