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Novel in vitro and in silico insights of the multi-biological activities and chemical composition of Bidens tripartita L.

dc.contributor.authorUysal S., Ugurlu A., Zengin G., Baloglu M.C., Altunoglu Y.C., Mollica A., Custodio L., Neng N.R., Nogueira J.M.F., Mahomoodally M.F.
dc.contributor.authorUysal, S, Ugurlu, A, Zengin, G, Baloglu, MC, Altunoglu, YC, Mollica, A, Custodio, L, Neng, NR, Nogueira, JMF, Mahomoodally, MF
dc.date.accessioned2023-05-09T16:01:57Z
dc.date.available2023-05-09T16:01:57Z
dc.date.issued2018-01-01
dc.date.issued2018.01.01
dc.description.abstractBidens tripartita L. is a traditional phyto-remedy used in several countries, yet there is still a paucity of data on its biological potential. We aimed to provide new insights on the pharmacological potential of extracts prepared from B. tripartita via highlighting its antioxidant, key enzymes inhibitory potency, and DNA protecting effects. Phytochemical profile was established using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) and bioactive compound(s) docked against target enzymes using in silico methods. Cytotoxicity against three cancer cell lines was assessed using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) cell viability test. The main compounds were luteolin-7-glucoside (cynaroside), chlorogenic acid, and epicatechin in the extracts. The methanol extract exhibited the highest radical scavenging activity. Ethyl acetate extract showed strongest α-amylase inhibitory activity, while the best α-glucosidase inhibitory effect recorded for the methanol extract. Molecular docking showed that cynaroside strongly interact to α-glucosidase cavity by establishing six hydrogen bonds. B. tripartita extracts were found to protect supercoiled form of pUC19 plasmid (>70%) and also showed anti-proliferative properties. Results amassed in the present study add on to a growing body of literature on the multi-pharmacological potency of B. tripartita which can be applied to bio-products development geared towards management of common diseases.
dc.identifier.doi10.1016/j.fct.2017.11.058
dc.identifier.eissn1873-6351
dc.identifier.endpage536
dc.identifier.issn0278-6915
dc.identifier.scopus2-s2.0-85037711133
dc.identifier.startpage525
dc.identifier.urihttps://hdl.handle.net/20.500.12597/12855
dc.identifier.volume111
dc.identifier.wosWOS:000423248100047
dc.relation.ispartofFood and Chemical Toxicology
dc.relation.ispartofFOOD AND CHEMICAL TOXICOLOGY
dc.rightsfalse
dc.subjectAnti-cancer | Anti-proliferative | Antioxidant | Cholinesterases | DNA protection | α-glucosidase
dc.titleNovel in vitro and in silico insights of the multi-biological activities and chemical composition of Bidens tripartita L.
dc.titleNovel in vitro and in silico insights of the multi-biological activities and chemical composition of Bidens tripartita L.
dc.typeArticle
dspace.entity.typePublication
oaire.citation.volume111
relation.isScopusOfPublication521f0ad3-d7e6-436a-8d5d-b909292c2f5d
relation.isScopusOfPublication.latestForDiscovery521f0ad3-d7e6-436a-8d5d-b909292c2f5d
relation.isWosOfPublication0d405949-63cf-4759-bcb2-9121798835a1
relation.isWosOfPublication.latestForDiscovery0d405949-63cf-4759-bcb2-9121798835a1

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