Pubmed İndekslenen Yayınlar

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12597/3195

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A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation
(2024) Ceylan, H.K.; Kırbay, F.Ö.; Yazgan, İ.; Elibol, M.
Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the circulating system are signs of pathology-associated diseases. Therefore, its precise and selective detection is critical for clinical applications to monitor the progression of the pathology. In this study, an optical immunoassay biosensor was developed as a model study for detecting recombinant VEGF. The VEGF sample was purified from recombinant Kluyveromyces lactis GG799 yeast cells. Indirect ELISA was used during the detection, wherein iron oxide nanoparticles (FeNPs) were utilized to obtain optical signals. The FeNPs were synthesized in the presence of lactose p-amino benzoic acid (LpAB). VEGF antibody was conjugated to the LpAB-FeNPs through EDC/NHS chemistry to convert the iron oxide nanoparticles into VEGF specific probes. The specificity of the prepared system was tested in the presence of potential serum-based interferents (i.e., glucose, urea, insulin, C-reactive protein, and serum amyloid A), and validation studies were performed in a simulated serum sample. The proposed immunoassay showed a wide detection range (0.5 to 100 ng/mL) with a detection limit of 0.29 ng/mL. These results show that the developed assay could offer a sensitive, simple, specific, reliable, and high-throughput detection platform that can be used in the clinical diagnostics of VEGF.
A system approach to the self: Interpretive phenomenological analysis
(2023) Erdoğan, Ö.
The study aims to determine the life experiences that affect the formation of permanent identity features that form the self during adolescence and to determine the importance of these identity features for adolescents. For this purpose, in-depth interviews were conducted with 8 participants, 4 of whom were women and 4 of whom were men, between the ages of 22-24, to understand the life experiences that affect their self-development during childhood and adolescence. Four of the participants are university students, and 4 of them are high school graduates. Interviews and analyses were carried out within the Interpretive phenomenological analysis principles framework. According to the results of the analysis obtained from the interviews, it was found that the most influential factor on self-development was family communication orientation in childhood, and the participants who grew up in families with a conversation orientation had more positive emotions during childhood, saw themselves as part of the family more, and established more successful friendships. On the other hand, participants from families with a conformity orientation had more negative feelings in childhood, had a lower sense of belonging to the family, and were found to be less successful in friendships.
A theoretical study on 1H-indole-2,3-dione complexes with lithium, sodium, and potassium cations
(2024) Genc, F.; Kandemirli, F.; Senturk Dalgic, S.
Context: A comparative study of the change in different properties of electronic and structural of the free 1H-indole-2,3-dione molecule and its complexes has been obtained. HOMA analysis was performed to investigate the effects of lithium sodium and potassium cations on the aromaticity of lithium sodium and potassium complexes of 1H-indole-2,3-dione. Methods: Several 1H-indole-2,3-dione complexes with lithium, sodium, and potassium cations were optimized at the B3LYP/6-311G(d,p) level. The cation and π interaction has been investigated from different aspects, including interaction energy calculations, charge transfer values, and changes in the aromaticity of the ring upon complexation. The charge transfer and natural population analysis for the complexes were performed with the natural bond orbital (NBO) analysis. The properties of bond critical points in complexes were studied by applying the quantum theory of atoms in molecules (QTAIM). Finally, the aromaticity change of phenyl induced upon complex formation was evaluated by applying the harmonic oscillator model of aromaticity (HOMA). [Li-INa]+ and [[Li-INb]+ were optimized with the wB97XD function using a version of Grimme's D2 dispersion model, and the absorption energy was compared with the calculation made with the B3LYP functional.
Altered luteal expression patterns of genomic and non-genomic progesterone receptors in bitches at different reproductive states
(2024) Ucar, E.H.; Peker, C.; Hitit, M.; Kose, M.; Tatar, M.; Bozkaya, F.; Atli, M.O.
The binding of steroid hormones to their specific receptors is necessary to exert their effects on target cells. Progesterone (P), a steroid hormone, carries out its effects through both genomic and non-genomic (the cell membrane-associated) receptors. This study aimed to ascertain luteal expression patterns of genomic and non-genomic progesterone receptors in bitches in physiological (early dioestrus and early pregnant) and pathological (pyometra) reproductive states. Luteal tissue was collected from the bitches at early dioestrus (ED, n = 5), early pregnant (EP, n = 5), and pyometra (PY, n = 5). The expression profiles of Steroidogenic Acute Regulator Protein (STAR), Progesterone Receptor (PGR), Membrane Progestin Receptors (PAQR5, PAQR7 and PAQR8), and Progesterone Membrane Components (PGMRC1 and PGMRC2) were examined at the mRNA levels using Real-Time Polymerase Chain Reaction (RT-PCR). Protein levels of PGR, PGMRC1 and PGMRC2 were detected by western blotting (WB). The STAR expression was found in all groups, with a statistical difference observed between EP and PY groups (P < 0.05). The protein level of PGR was determined to be highest in the EP group and lowest in the PY group. The expression of PAQR8 increased in the EP group (P < 0.05). The PAQR5 exhibited high expression in the EP group and low expression in the PY group (P < 0.05). PGRMC1 was more elevated in the EP group and lower in the PY group (P < 0.05). Protein levels of PGMRC1 and PGMRC2 were also observed at the highest expression in EP group. According to the altered expression profiles for examined receptors, we suggest that those progesterone receptors have roles in early pregnancy or pyometra in bitches.
Ameliorative effects of humic acid and L-tryptophan on enzyme activity, mineral content, biochemical properties, and plant growth of spinach cultivated in saline conditions
(2024) Turfan, N.; Kibar, B.; Davletova, N.; Kibar, H.
Salinity poses a significant abiotic stress that limits plant productivity, thereby posing a serious threat to agricultural sustainability and worldwide food security. Techniques that can overcome this problem are needed. Recent focus has been placed on employing organic substances like humic acid (HA) and amino acids, including L-tryptophan (L-TRP), to mitigate the negative effects of salt stress on cultivated plants. Accordingly, in this research, the impact of foliar applications of HA and L-TRP, both separately and combined, on the growth parameters and biochemical properties of spinach subjected to salt stress was investigated. In the present study, eight treatments (1. control, 2. salt (NaCl), 3. HA, 4. L-TRP, 5. HA + NaCl, 6. L-TRP + NaCl, 7. HA + L-TRP, and 8. HA + L-TRP + NaCl) were investigated. The study showed that salt stress markedly reduced several growth properties in spinach, including plant height, number of leaves, leaf dimensions, and both fresh and dry weight. Additionally, it significantly lowered contents of chlorophyll (, , and total), carotenoid, polyphenol, lutein, anthocyanin, polyphenol oxidase, glycine betaine, relative water content, and the antioxidant enzyme activities (ascorbate peroxidase, catalase, peroxidase, and superoxide dismutase). On the other hand, significant increases were observed in sodium, chlorine, potassium, sulfur, zinc, nickel, proline, malondialdehyde, and hydrogen peroxide levels of spinach with salinity. Individual and combined applications of HA and L-TRP positively influenced plant growth, relative water content, activities of antioxidant enzyme, chlorophyll, and mineral contents of spinach under both normal and saline conditions. In conclusion, the combined use of HA and L-TRP under salt stress conditions is promising in mitigating the negative impacts of salinity and can be suggested as an effective alternative approach for cultivating spinach in saline environments.
An analysis of the relationship of "the Mozart effect" with BDNF levels in anatomy education
(2024) Petekkaya, E.; Ünalmış Aykar, D.; Kaptan, Z.
In 1993, an increase was observed in the spatial IQ scores of the volunteers who listened to Mozart's sonata K448 for 10 min, and this phenomenon entered the literature as the "Mozart effect." Other studies have shown that this effect is particularly evident in spatial skill tests. A large body of research has provided evidence that spatial ability is associated with success in learning anatomy. In this study, Kastamonu University Faculty of Medicine students were divided into two groups during 16-h practical training spanning 30 days. While one of the groups listened to Mozart's K448 sonata as the background music in all lessons, the control group attended the lessons in their standard form. At the end of each lesson, all students solved a modified mental rotation test including questions involving anatomical structures. Before starting the study, after the first laboratory class, on the 15th and 30th day of the study, blood samples were taken from the participants, and plasma brain-derived neurotrophic factor (BDNF) levels were determined. The effect of time on mental rotation score and plasma BDNF level was significant (p < 0.001 for both). The effect of group was also significant (p < 0.001 for both). Pairwise comparisons showed significance in the fifth, sixth, seventh, and eighth mental rotation test (p < 0.001, p = 0.041, p < 0.001, p < 0.001, respectively) and in the third (Day 15) and fourth (Day 30) BDNF measurement (p < 0.001 for both). Our findings may indicate that specific background music may be useful for anatomy teaching.
An investigation of the distributions of ferroptosis and necroptosis mediators in the maternal-fetal interface at different days of rat pregnancy
(2023) Tatar, M.; Tüfekci, K. K.
Ferroptosis and necroptosis are recognized as playing major roles in the regulation of various physiological processes. However, the physiological role of the cell death mediated by these two pathways in the developmental process has not yet been clearly established. This study investigated ferroptosis and necroptosis signalling pathways in maternal-fetal tissue in the different gestational days (GD) of rat pregnancy using immunohistochemical and western blot methods in order to fill this gap. Twenty-four female Wistar albino rats were mated and divided into three groups. Maternal-fetal tissue samples were collected on GD 5, 12 and 19 of pregnancy. Expression and total protein levels of the markers glutathione peroxidase-4, soluble transporter family 7 member 11, transferrin receptor, receptor-interacting serine/threonine-protein kinase 1, receptor-interacting serine/threonine-protein kinase 3 and mixed lineage kinase domain-like protein were investigated on both the maternal and fetal surfaces of the placenta using immunohistochemical and western blot methods. The results showed varying levels of protein expression of both ferroptosis and necroptosis mediators in the GD 5, 12 and 19 of pregnancy. Immunohistochemical analyses revealed that these mediators were located on both the maternal (decidua and metrial gland) and fetal surfaces (labyrinth zone, yolk sac and basal zone) and that their expression levels changed in the different GD. The findings revealed the existence of important ferroptosis and necroptosis pathway mediators in rat maternal-fetal tissue. These results may provide a molecular framework for a better understanding of the communication between the placenta, decidua and fetus during the developmental process.
An investigation of the endoplasmic reticulum stress in obesity exposure in the prenatal period
(Elsevier, 2023) Tüfekci, K.K.; Tatar, M.; Terzi, F.; Bakirhan, E. G.
Objectives: Exposure to maternal obesity has been shown to make offspring more prone to cognitive and metabolic disorders later in life. Although the underlying mechanisms are unclear, the role of endoplasmic reticulum (ER) stress in the fetal programming process is remarkable. ER stress can be activated by many chronic diseases, including obesity and diabetes. Therefore, our study aimed to investigate the role of ER stress caused by maternal diet-induced obesity in the offspring hippocampus. We also evaluated the protective effect of N-acetylcysteine (NAC) against ER stress. Methods: A rat obesity model was created by providing a high-fat (60% kcal) diet. Nacetylcysteine (NAC) was administered at a dosage of 150 mg/kg via the intragastric route. The animals were mated at the age of 12 weeks. The same diet was maintained during pregnancy and lactation. The experiment was terminated on the postnatal 28th day, and the offspring's brain tissues were examined. Immunohistochemical staining for ER stress markers was performed on sections taken from tissues after routine histological procedures. Results: The results revealed increased GRP78, PERK, and eIF2α immunoreactivities in the hippocampal dentate gyrus (DG) and cornu ammonis 1 (CA1) regions in the obese group offspring, while the expression of those markers in those regions normalized with NAC supplementation (p<0.01). Statistical analysis of XBP1 immunoreactivity H-scores revealed no difference between the study groups (p>0.05). Discussion: These results suggest that exposure to obesity during the prenatal period may cause increased ER stress in hippocampal neurons, which have an important role in the regulation of learning, memory and behavior, and this may contribute to decreased cognitive performance. On the other hand, NAC stands out as an effective agent that can counteract hippocampal ER stress.
Anatomical and Functional Results of Early or Late Switching from Anti-VEGF to Dexamethasone Implant in Case of Poor Anatomical Response in Naïve Patients with Macular Edema Secondary to Branch Retinal Vein Occlusion
(2024) Yozgat, Z.; Işik, M.U.
Purpose: To compare the outcomes of early or late switching from intravitreal (IV) anti-vascular endothelial growth factor (anti-VEGF) injection to IV Dexamethasone (DEX) implant injection in treatment-naïve patients with macular edema secondary to branch retinal vein occlusion. Methods: This study included 68 eyes of 68 treatment-naïve BRVO patients who started anti-VEGF treatment. After the loading dose, the patients were divided into two groups: Early DEX group (n:34) (DEX implant treatment started after 3 loading doses) and Late DEX group (n:34) (DEX implant treatment started after 6 months). Visual acuity and examination findings were recorded at baseline, 3rd, 6th, and 12th month follow-ups. Optical coherence tomography data were recorded for central macular subfield thickness assessment. Results: A total of 30 (44.1%) women and 38 (55.9%) men participated, and the average age was 67.6 ± 6.4 years. The mean letter gains at week 52 was 15.1 and 20.9 in the Early DEX and Late DEX groups, respectively. The group with the highest gain of ≥15 letters was the Late DEX group (26/34 patients) and the gain of ≥15 letters was 14/34 in the Early DEX group (p: 0.006). At week 52, the anatomical gain was 115.3 µm and 136.9 µm in the Early DEX and Late DEX groups, respectively. Conclusions: A gain of 15 or more letters was demonstrated to be higher in patients who switched to DEX implant late after anti-VEGF treatment. If it is necessary to switch, the late switch may be more effective for more visual gain at the end of the first year.
Are Preoperative CT Findings Useful in Predicting the Duration of Laparoscopic Appendectomy in Pediatric Patients? A Single Center Study
(2024) Taskent, I.; Ece, B.; Narsat, M.A.
Preoperative computed tomography (CT) imaging plays a vital role in accurately diagnosing acute appendicitis and assessing the severity of the condition, as well as the complexity of the surgical procedure. CT imaging provides detailed information on the anatomical and pathological aspects of appendicitis, allowing surgeons to anticipate technical challenges and select the most appropriate surgical approach. This retrospective study aimed to investigate the correlation between preoperative CT findings and the duration of laparoscopic appendectomy (LA) in pediatric patients. This retrospective study included 104 pediatric patients diagnosed with acute appendicitis via contrast-enhanced CT who subsequently underwent laparoscopic appendectomy (LA) between November 2021 and February 2024. CT images were meticulously reviewed by two experienced radiologists blinded to the clinical and surgical outcomes. The severity of appendicitis was evaluated using a five-point scale based on the presence of periappendiceal fat, fluid, extraluminal air, and abscesses. The average operation time was 51.1 ± 21.6 min. Correlation analysis revealed significant positive associations between operation time and neutrophil count ( = 0.014), C-reactive protein levels ( = 0.002), symptom-to-operation time ( = 0.004), and appendix diameter ( = 0.017). The total CT score also showed a significant correlation with operation time ( < 0.001). Multiple regression analysis demonstrated that a symptom duration of more than 2 days ( = 0.047), time from CT to surgery ( = 0.039), and the presence of a periappendiceal abscess ( = 0.005) were independent predictors of prolonged operation time. In the perforated appendicitis group, the presence of a periappendiceal abscess on CT was significantly associated with prolonged operation time ( = 0.020). In the non-perforated group, the presence of periappendiceal fluid was significantly related to longer operation times ( = 0.026). In our study, preoperative CT findings, particularly the presence of a periappendiceal abscess, were significantly associated with prolonged operation times in pediatric patients undergoing laparoscopic appendectomy. Elevated CRP levels, the time between CT imaging and surgery, and a symptom duration of more than 2 days were also found to significantly impact the procedure's duration.
Assessment of metals (Ni, Ba) deposition in plant types and their organs at Mersin City, Türkiye
(2024) Koç, İ.; Canturk, U.; Isinkaralar, K.; Ozel, H.B.; Sevik, H.
The increase in heavy metal concentrations in the air, especially after the Industrial Revolution, is notable for the scientific world because of the adverse effects that threaten environmental and human health. Among the trace elements, nickel (Ni) is carcinogenic, and all barium (Ba) compounds are toxic. Trace elements are critical for human and environmental health. Their threat further increases, especially in the urban areas and surroundings with a high population. In urban areas, the trace element contamination in the airborne can be reduced using plants. However, which plant and plant organs absorb trace elements could not be determined. In the present study, Ni and Ba concentrations in the branch, wood, and leaf samples of 14 species collected from the city center of Mersin province were determined. As a result, broad-leaved species' Ni and Ba concentrations in their leaf sample were generally higher than other species. Almost all species had the lowest Ni and Ba concentrations in their wood samples. Among these 14 species, it was found that Ni concentration was very high, especially in non-washed leaves of Platanus orientalis, Photinia serrulata, and Citrus reticulate, and Ba concentration was very high in Citrus reticulata, Chamaecyparis lawsoniana, Laurus nobilis, and Acer hyrcanum. Using broad-leaved species in urban areas where pollution is at high levels will significantly contribute to reducing Ni and Ba pollution. It is recommended that these points be considered in future urban landscaping projects.
Assessment of Psoas Muscle Index in Middle-Aged Type 2 Diabetes Patients: Impact of Insulin Therapy on Sarcopenia
(2024) Taskent, I.; Ece, B.; Aydin, S.
Objective: Sarcopenia, characterized by progressive skeletal muscle loss, poses significant health risks, including physical impairment and mortality. The relationship between sarcopenia and insulin resistance suggests insulin therapy's potential in preserving muscle mass, particularly in Type 2 diabetes mellitus (T2DM) patients. This study aims to evaluate the Psoas Muscle Index (PMI) via computed tomography (CT) in middle-aged T2DM patients on insulin therapy versus oral antidiabetic drugs (OAD) and controls. Methods: This retrospective study included 107 middle-aged T2DM patients undergoing non-contrast CT scans and 58 age-matched controls. CT images were analyzed to calculate PMI. Statistical analysis included Chi-square tests, independent samples t-tests, Mann-Whitney U tests, and correlation analyses. Results: Insulin-treated patients exhibited higher PMI than OAD users (p < 0.001), while OAD users had lower PMI than controls (p < 0.001). No significant difference was found between insulin-treated patients and controls (p = 0.616). Negative correlations were observed between T2DM duration/age and PMI across all groups, with a positive correlation between T2DM duration and BMI observed in the OAD group. Conclusions: Insulin therapy in T2DM patients, regardless of age or disease duration, positively impacts muscle mass, highlighting its potential in preserving muscular health and advocating for tailored treatment strategies in T2DM management.
Biomonitoring with the Use of the Herbal Plant Taraxacum officinale as a Source of Information on Environmental Contamination
(2024) Respondek, Z.; Isinkaralar, O.; Świsłowski, P.; Isinkaralar, K.; Rajfur, M.
The aim of this study was to assess the level of contamination of the common dandelion--with selected metals (Mn, Fe, Ni, Cu, Zn, Cd, and Pb) and to demonstrate that this plant can be used in passive biomonitoring of industrial sites. Two sample transects (the first was near a forest, an area potentially uncontaminated by analytes [A], while the second ran near a steel mill, a contaminated area [B]), each about 1.5 km long, located in Ozimek, Opole Province, Poland, were used in this study. Metals in plant and soil samples were determined by atomic absorption spectroscopy (AAS). Based on the analysis of the obtained results to determine the concentration of metals, plants at site A were more contaminated with Mn (240 mg/kg d.m.) and those at site B with Fe (635 mg/kg d.m.). Mean Pb values (8.39 mg/kg d.m.) were higher at the industrial site (B) and statistically significant at the forest site (A), together with Mn and Fe at the < 0.001 level. The values for showed that Cu (0.473) and Zn (0.785) accumulated to an average degree on both transects. This shows that dandelion is heavily loaded with these metals. Both dandelion and soil samples showed the highest concentrations of Mn, Fe, and Zn, especially in the polluted area B, which is the result of pollution not only from the smelter (dust from electric arc furnaces in steel smelting, extraction installations in production halls transmitting pollutants into the air from molding sand, or waste from molding and core masses dumped on the heap and blown by the wind from the landfill) but also from the high anthropopressure caused by human activity-for example, heating processes or road transport. Our results confirmed that can be successfully used as a herbal plant in passive biomonitoring to assess the quality of the environment, but it must be collected from uncontaminated areas if we want to use it like a medicinal plant.
Can highway tunnel constructıon change the habitat selection of roe deer (Capreolus capreolus Linnaeus, 1758)?
(2023) Evcin, Ö.
One of the main things wildlife does for survival is movement. Wild animals need movement to meet their needs, such as reproduction, breeding, foraging, and dispersal. Although wildlife species use roads for various purposes, they also use them when moving from one habitat to another. In recent years, especially when it comes to habitat fragmentation brought about by urbanization, wild animals frequently use highways. Highways have a wide range of effects on factors such as biodiversity, wildlife, and ecology. Roads can cause habitat loss, habitat fragmentation, and habitat degradation; alter the composition of vegetation; act as barriers to the flow of genes and movement; increase human access to pristine areas; and even increase the risk of extinction for many threatened species. Species belonging to the family Cervidae also include the species most affected by road networks. Roe deer (Capreolus capreolus Linnaeus, 1758) is the smallest of the 3 Cervid species living in Turkey. Roe deer are often injured or die in road accidents, and they are one of the most important species affected by the adverse effects of roads in Turkey. For this reason, it was investigated whether the road tunnel construction affected the distribution of roe deer in the region. In the study, the general distribution of roe deer in the Ilgaz Mountain, and the factors affecting their possible distribution were determined by ecological niche modeling. Data were taken between before (2012-2015) and after the highway tunnel built (2020-2022) in Ilgaz Mountain, which connects the Western Black Sea and Central Anatolia and is located in the middle of Kastamonu and Çankırı provinces. As a result of the modeling, it was found that before the construction of the tunnel, the most influential factor in the distribution of the deer was road density. After the tunnel construction, roads ceased to be the main factor affecting the distribution of the species. This study showed that roe deer are disturbed by the density of vehicles on the road passing through the middle of their habitat. With the decrease in the number of vehicles, they are more willing to cross the road and tend to use the areas close to the road as they are less disturbed.
Care burden and resilience in parents of children with special needs and chronic diseases
(2024) Kaş Alay, G.; Kaçan, H.
Aim: Comparison of care burden and resilience levels in parents of children with special needs and chronic diseases. Methods: This study, designed with a cross-sectional and relationship-seeking design, was conducted in a Training and Research Hospital in Turkey. Data was obtained from parents of children with special needs (n = 102) and chronic diseases (n = 102) who presented to the outpatient clinic for routine follow-up between October 2022 and February 2023. The consecutive sampling method was used while choosing the sample. The "Descriptive Characteristics Questionnaire," "Zarit Caregiver Burden Scale (ZCB)," and "Resilience Scale for Adults (RSA)" were used to collect data. Data were evaluated using the SPSS 22.0 statistical program. Results: The burden of care in parents differed significantly between the two groups (p < 0.001), the majority of parents of children with chronic diseases (PCCD) had a mild/moderate care burden, and the majority of parents of children with special needs (PCSN) had a moderate/severe level of care. The care burden scores and total resilience scores were found to be higher in the PCSN group, and the care burden scores and total resilience scores were found to be lower in the PCCD group (p < 0.001). Conclusion: This research determined that PCSN showed more care burden and less resilience than PCCD. In this context, it is recommended to regularly monitor the resilience, caregiver burden, and quality of life of caregivers of children with special needs and conduct preventive and strengthening programs.
Carotenoid characterization, fatty acid profiles, and antioxidant activities of haloarchaeal extracts
(2023) Kesbiç, F. I.; Gültepe, N.
Microorganisms that can survive in saline environments, known as halotolerant or halophilic organisms, have a wide range of current and potential uses in biotechnology. In this study, it was aimed to determine the carotenoids of halophilic archaea strains isolated from the brine samples taken from different points of Salt Lake (Turkey) and determine the antioxidant activities of their carotenoids. To identify the halophilic archaea strains, they were cultivated in MAM JCM 168 medium and subjected to antibiotic susceptibility, fatty acid, two-dimensional and three-dimensional imaging by scanning electron microscopy and atomic force microscopy, biochemical and phylogenetic assays. The findings show that five different halophilic archaea strains have been identified as Halorubrum lipolyticum, Halorubrum sodomense, Haloarcula salaria, Halorubrum chaoviator, and Haloarcula japonica with 98% and above similarity ratio. The main fatty acids of all haloarchaeal strains were octadecanoic acid (C18:0) and palmitic acid (C16:0). The major carotenoid of the species was determined as all-trans bacterioruberin, and different carotenoid types such as lycopene, β-carotene, and 2-isopentenyl-3,4-dehydrorodopin were found as well as bacterioruberin isomers. The antioxidant activities of carotenoids extracted from the species were analyzed by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging method and the extracts showed antioxidant activity statistically significantly higher than ascorbic acid and butylated hydroxytoluene as reference products (p < 0.05).
Chronotype, sleep quality, impulsivity and aggression in patients with borderline personality disorder and healthy controls
(2024) Taşdelen, Y.; İnaltekin, A.
Impulsivity, aggression, and suicide are the major clinical symptoms of borderline personality disorder (BPD). Although previous studies indicated poor sleep quality and its relationship with clinical symptoms in patients with BPD, chronotype, an important sleep parameter, was not investigated in these patients. This study aimed to analyze chronotype and its relationship with clinical symptoms in patients with BPD. Participants in this study consisted of 68 BPD patients and 65 healthy controls. Subjective sleep characteristics, impulsivity, aggression, suicide probability, and chronotype were assessed using the Pittsburgh Sleep Quality Index (PSQI), Barratt Impulsivity Scale, Buss-Perry Aggression Scale (BPAQ), Suicide Probability Scale, and Morningness - Eveningness Questionnaire, respectively. PSQI total and subscale scores subjective sleep quality, sleep latency, sleep duration, habitual sleep efficiency, sleep disturbances, and daytime drowsiness were significantly higher in the BPD group ( < 0.001). There was a positive correlation between the PSQI total score and the BPAQ total score ( = 0.268, = 0.027). The rate of evening type was significantly higher in the control group ( = 0.004). Suicide attempts and the subscale of suicide probability hopelessness, suicidal ideation, and negative self-evaluation scores were significantly higher in evening type BPD patients. ( = 0.017, = 0.009, = 0.001, = 0.047). Sleep quality is associated with aggression, and the eveningness chronotype is associated with suicide. It may be useful to focus on sleep problems in treating BPD patients.
Cochlear Synaptopathy Evaluation With Electrocochleography in Patients With Hearing Difficulty in Noise Despite Normal Hearing Levels
(2024) Yaşar, M.; Öner, F.; Atalay, F.; Anbar, S.S.
Objective: This study examined patients with normal hearing thresholds who had trouble understanding speech in noise. We used electrocochleography (ECochG) to detect and compare SP/AP amplitude area ratios, a potential indicator of cochlear synaptopathy, and investigate speech perception disorder in noise. Methods: The study included 68 people aged between 18 and 65 years, 35 patients and 33 healthy volunteers, who applied to the otorhinolaryngology clinic between November 2023 and March 2024 with a 2-month history of difficulty understanding speech in noisy environments. Everyone was given a tiptrode electrode ECochG test, and the results were compared between groups. An ECochG test was recorded with tiptrode electrodes and was performed on all participants, and the results were compared between groups. Results: In the ECochG test, the summation potential/action potential (SP/AP) amplitude and area ratios of patients who had difficulty understanding speech in a noisy environment were statistically higher than those of the control group. Conclusion: ECochG testing may provide additional evidence to evaluate auditory nerve pathways.
Comparative analysis of biometrical and reproductive indices, proximate composition, and hemato-biochemical variables of cuchia eel Monopterus cuchia (Hamilton, 1822) from six different localities of Bangladesh
(2024) Nandi, S.K.; Al Mamun, M.A.; Suma, A.Y.; Abdul Kari, Z.; Wei, L.S.; Tahiluddin, A.B.; Manjappa, N.K.; Nasren, S.; Saha, S.; Téllez-Isaías, G.; Bottje, W.G.; Kabir, M.A.
Cuchia eel () is among the most sought-after freshwater fish, owing to its exceptional nutritional profile and high consumer demand. The current research aimed to establish baseline data by comparing the proximate composition, hematological, and plasma biochemical indices of Cuchia eel populations across six different geographical locations in Bangladesh: Bogra, Haluaghat, Jamalpur, Moktagacha, Sylhet, and Tangail. By examining these parameters, we aim to gain valuable insights into the nutritional benefits, physiological responses, and potential adaptations of this species to varying environments. The statistical analysis revealed no significant ( > 0.05) variances in the whole-body proximate composition of the fish captured from distinct areas. However, it was observed that different geographical regions had remarkable impacts on the variations of the majority of the hematological parameters, except for some cases. Additionally, there was a notable ( < 0.05) increase or decrease in most of the serum biochemical contents in certain localities as compared to others in this study. Light microscopic examination of Cuchia eel blood smears exhibited lower numbers but larger sizes of RBCs. The findings of this study lead to the conclusion that different localities had significant impacts on the hematology and blood biochemical indices of Cuchia eel, even though the whole-body proximate composition showed no significant variations. This research contributes to a deeper understanding of the physiological aspects of Cuchia eel.
Comparison of Resorption in Autogenous Dorsal Onlay Cartilage Grafts: An Experimental Study
(2024) Öner, F.; Kozan, G.
Objectives: The present study was designed to compare the graft resorption characteristics of autogenous cartilage from the septum, auricle, and costal in the superficial muscular aponeurotic system of the nasal dorsum of the rabbit model. Methods: Equal-sized perichondrium-free septal, auricular, and costal cartilage grafts were collected from fifteen New Zealand white rabbits. Cartilage grafts were taken at the scale of two grafts from each animal's ear, two from its costal part, and one from its septum. Costal cartilage grafts that were shaped with a micro-motor device and monopolar electrocautery, elastic cartilage grafts that were shaped with a micro-motor device and monopolar electrocautery, and septal cartilage grafts that were shaped with a scalpel were all implanted into the dorsum of rabbit's noses to create five groups. All autogenous cartilage tissues were removed 3 months later. Cartilages were evaluated for histological features, graft mass, and chondrocyte density resorption. Results: The elastic cartilage group, where electrocautery was used to shape the cartilage, had a higher resorption score than the other groups. The costal cartilage graft shaped with a micro-motor was also observed to have the best cartilage regeneration score. Conclusion: We observed that the resorption of costal cartilage was lower than that of ear and septum cartilage. It was determined that micro-motor application for the shaping process caused less resorption and stimulated more regeneration than cautery application. Level of evidence iii: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

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