Araştırma Çıktıları

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(1 → 3)-β-d-glucan enhances the toxicity induced by Bortezomib in rat testis.
(2020-03-01T00:00:00Z) Akaras, Nurhan; Abuc, Ozlem Ozgul; Koc, Kubra; Bal, Tugba; Geyikoglu, Fatime; Atilay, Hilal; Erol, Huseyin Serkan; Yigit, Serdar; Gul, Murat
We aimed to determine the possible effects of the antioxidant agent (1 → 3)-β-D-glucan on bortezomib-induced rat testis damage. We used five groups of rats; control, (1 → 3)-β-D-glucan (75 mg/kg), bortezomib group, bortezomib + (1 → 3)-β-D-glucan groups (injection of (1 → 3)-β-D-glucan after bortezomib and sacrificed at 48th or 72nd h). The effects of these substances were assessed by measuring the levels of the antioxidant enzymes and LPO, and by performing immunohistochemical analysis with NF-κB. The histology of testis was evaluated using aniline blue staining. (1 → 3)-β-D-glucan leads to significant reductions in the levels of antioxidant enzymes and increased levels of LPO in testes. Moreover, it increased the NF-κB immunopositivity significantly in testis, especially in Bortezomib + (1 → 3)-β-D-glucan group at 48th h. The histological changes were observed in the bortezomib and/or (1 → 3)-β-D-glucan groups. Our results demonstrated that testis damage caused by the treatment with bortezomib was not eliminated by (1 → 3)-β-D-glucan and shockingly it increased the damage. PRACTICAL APPLICATIONS: The testis damage caused by the treatment with bortezomib was not eliminated by (1 → 3)-β-D-glucan and as a result, β-1,3-(D)-glucan enhanced the toxicity by leading a decrease in the levels of GSH, SOD, and CAT, thus caused an elevation in the immunoreactivity of NF-κB and altered the histopathological changes by enhancing the toxic effects of bortezomib. The findings of the previous studies about the antioxidative activity of (1 → 3)-β-D-glucan are controversial. So, it is necessary to consider the cytotoxicity of (1 → 3)-β-D-glucan in testis tissue. Thus, more studies on testis tissue are necessary to confirm that (1 → 3)-β-D-glucan is safe as an antioxidant.
1,2,3-Triazole substituted phthalocyanine metal complexes as potential inhibitors for anticholinesterase and antidiabetic enzymes with molecular docking studies.
(2022-07-01T00:00:00Z) Koçyiğit, Ümit M; Taslimi, Parham; Tüzün, Burak; Yakan, Hasan; Muğlu, Halit; Güzel, Emre
In recent years, acetylcholinesterase (AChE) and α-glycosidase (α-gly) inhibition have emerged as a promising and important approach for pharmacological intervention in many diseases such as glaucoma, epilepsy, obesity, cancer, and Alzheimer's. In this manner, the preparation and enzyme inhibition activities of peripherally 1,2,3-triazole group substituted metallophthalocyanine derivatives with strong absorption in the visible region were presented. These novel metallophthalocyanine derivatives () effectively inhibited AChE, with values in the range of 40.11 ± 5.61 to 78.27 ± 15.42 µM. For α-glycosidase, the most effective values of compounds and were with values of 16.11 ± 3.13 and 18.31 ± 2.42 µM, respectively. Also, theoretical calculations were investigated to compare the chemical and biological activities of the ligand () and its metal complexes (-). Biological activities of and its complexes against acetylcholinesterase for ID 4M0E (AChE) and α-glycosidase for ID 1R47 (α-gly) are calculated. Theoretical calculations were compatible with the experimental results and these 1,2,3-triazole substituted phthalocyanine metal complexes were found to be efficient inhibitors for anticholinesterase and antidiabetic enzymes.Communicated by Ramaswamy H. Sarma.
3-({4-[(2-Methyl-benzyl-idene)amino]-5-sulfanyl-idene-1H-1,2,4-triazol-3-yl}meth-yl)-1,3-benzoxazol-2(3H)-one.
(2013-02-01T00:00:00Z) Aydın, Abdullah; Hekimoğlu, Nuray; Akkurt, Mehmet; Onkol, Tijen; Ciçekli, Sölen Urlu; Büyükgüngör, Orhan
In the title compound, C(18)H(15)N(5)O(2)S, a weak intra-molecular C-H⋯S hydrogen bond results in a small dihedral angle of 3.71 (9)° between the methyl-phenyl and triazole rings, which, in turn, form dihedral angles of 80.09 (8) and 77.32 (8)°, respectively, with the benzoxazolone mean plane. In the crystal, N-H⋯O hydrogen bonds link mol-ecules into chains along [001], and weak C-H⋯N hydrogen bonds and π-π inter-actions between the five- and six-membered rings [centroid-centroid distances = 3.5074 (11) and 3.616 (1) Å] consolidate the crystal packing.
3-D Mental Rotation Ability Testing with Mixed Reality
(2023.01.01) Piri, Z.; Cagiltay, K.
Mental Rotation ability is a key skill for success in many careers. Thus, accurate measurement of this ability is important. Three-dimensional holographic displays can help students process mental rotation stimuli easier and demonstrate their real performances without the limitations of two-dimensional representations. There are few studies measuring mental rotation ability in Mixed Reality environments. In this study, we transferred a mental rotation test, Purdue Spatial Visualization Test: Rotations (PSVT:R), into Mixed Reality. We compared the 2-D and 3-D versions of the test in terms of mental rotation performance and cognitive load with 47 participants. While the accuracy of 2-D and 3-D PSVT:R did not differ significantly, the 3-D test induced a lower cognitive load than the 2-D version. Gender difference was observed in the 2-D test but not in the 3-D test. 3-D PSVT:R is an authentic and reliable tool for measuring spatial ability. Moreover, it can be used for training purposes.
3D numerical analysis of a Li-ion battery cooling system with honeycomb configuration in electrical vehicles
(Elsevier B.V., 2024) Nazlı, C.; Gürdal, M.; Arslan, K.
This study focuses on the thermal challenges faced by lithium-ion batteries in electric vehicles and the importance of effective thermal management systems. It has been conducted a 3D numerical analysis to investigate the impact of different distances between batteries on heat transfer and flow characteristics in an air-cooled cooling module with an innovative honeycomb configuration. Boundary conditions and the study results were given with dimensionless parameters with longitudinal ratio (x/λ), distance ratio (λ), and height ratio (y/H). It was found that smaller distances between batteries let to higher temperatures at the beginning of the cooling channel due to the shorter channel width. However, as the distance between batteries increases, convective heat transfer performance improves, resulting in better temperature distributions and higher Nusselt numbers. The results shed light on the importance of optimizing the spatial arrangement of batteries in a cooling module to achieve efficient thermal management. This research contributes to bridging existing gaps in knowledge regarding battery thermal management in electric vehicles and provides insights for the design and development of more effective cooling strategies for lithium-ion batteries. In conclusion, according to the distance between batteries (S=1–3-5 mm) under a laminar flow regime, the best average Nussell numbers obtained for S=5 mm %17 and %7 heat enchantments compared to other S=1 mm and S=3mm cases, respectively. It can be observed that the convective heat transfer performance is optimal when all cases are located on the 9th battery. After the 9th battery column, the optimal heat transfer performance was observed for a thickness of 5 mm. The S=3 mm case exhibited a 7 % reduction in convective heat transfer performance compared to the S=5 mm case.
3D numerical analysis of a Li-ion battery cooling system with honeycomb configuration in electrical vehicles
(2024.01.01) Nazli, C.; Gürdal, M.; Arslan, K.
This study focuses on the thermal challenges faced by lithium-ion batteries in electric vehicles and the importance of effective thermal management systems. It has been conducted a 3D numerical analysis to investigate the impact of different distances between batteries on heat transfer and flow characteristics in an air-cooled cooling module with an innovative honeycomb configuration. Boundary conditions and the study results were given with dimensionless parameters with longitudinal ratio (x/lambda), distance ratio (lambda), and height ratio (y/H). It was found that smaller distances between batteries let to higher temperatures at the beginning of the cooling channel due to the shorter channel width. However, as the distance between batteries increases, convective heat transfer performance improves, resulting in better temperature distributions and higher Nusselt numbers. The results shed light on the importance of optimizing the spatial arrangement of batteries in a cooling module to achieve efficient thermal management. This research contributes to bridging existing gaps in knowledge regarding battery thermal management in electric vehicles and provides insights for the design and development of more effective cooling strategies for lithium-ion batteries. In conclusion, according to the distance between batteries (S=1-3-5 mm) under a laminar flow regime, the best average Nussell numbers obtained for S=5 mm %17 and %7 heat enchantments compared to other S=1 mm and S=3mm cases, respectively. It can be observed that the convective heat transfer performance is optimal when all cases are located on the 9th battery. After the 9th battery column, the optimal heat transfer performance was observed for a thickness of 5 mm. The S=3 mm case exhibited a 7 % reduction in convective heat transfer performance compared to the S=5 mm case.
5-HT7 receptors as a new target for prostate cancer physiopathology and treatment: an experimental study on PC-3 cells and FFPE tissues.
(2021-06-01T00:00:00Z) Cinar, Irfan; Sirin, Busra; Halici, Zekai; Palabiyik-Yucelik, Saziye Sezin; Akpinar, Erol; Cadirci, Elif
Prostate cancer (PCa) is one of the most common types of cancer seen among men worldwide. Previous studies have demonstrated that serotonin regulates cell proliferation, migration, and invasion in vitro; the presence of 5-HT receptors in cancer cells; and the role of serotonin in tumor development. The most recently discovered of these receptors is 5-HT7 but also least characterized receptors of serotonin. The aim of this study is to investigate the existence and possible role of 5-HT7 receptors in healthy and cancerous prostate tissues and also investigate effects of receptor agonists and antagonists on PC-3 cells to evaluate potential therapeutic effects. PC-3 cells were cultured and effects of 5-HT7 receptor agonist (LP-44) and antagonist (SB-269970) were evaluated on these cells. After proliferation analyses, relative expression of apoptotic markers and 5-HT7 receptor mRNA expression levels were determined through real-time PCR. Annexin V-FITC/PI double staining and Hoechst 33258 staining assay methods were applied to determine apoptosis. Additional PCR studies were performed on healthy and cancerous prostate tissue to see existence of receptors in human samples. The viability of PC-3 cells was decreased by SB-269970 after 48 and 72 h of incubation. However, LP-44 increased PC-3 cell proliferation at all time points. In 10 M SB-269970 treated PC-3 cells, there was significant increase in the expression of CAS-3 (4-fold), CAS-9 (2.5-fold), BAX (1.9-fold), and Tp-53 (4.8-fold) gene mRNA levels when compared to non-treated control group. Conversely, there was a significant decrease in NF-κB (2.9-fold) and 5-HT7 receptor (3.6-fold) mRNA expression in cells treated with SB-269970 when compared to control. SB-269970 that antagonized 5-HT7 receptors also induced apoptosis in Annexin V-FITC/PI double staining assay and Hoechst 33258 staining assays when compared with other groups. In human samples, 5-HT7 receptor mRNA expression was approximately 200-fold higher than that of heathy ones. In this study, for the first time, the 5-HT7 receptor antagonist SB-269970 has been shown to inhibit proliferation in PC-3 cells and to be associated with an apoptosis-inducing effect. These results suggest blocking 5-HT7 receptors can be a novel therapeutic target for the treatment of prostate cancer.
A Bibliometric Analysis of Scientific Research in Türkiye on National Parks Between 2002 and 2021
(Istanbul University-Cerrahpasa, 2024) Yelsiz, M.Ş.; Yücedaǧ, C.; Çiçek, N.; Ayan, S.
In Türkiye, national parks play a prominent role among protected areas. Besides meeting people's recreational needs, these protected areas are home to natural and cultural resources. As a result, numerous research has been conducted to find out more about assets and recreational uses of these areas. In this study, the bibliometric analysis method was used to examine research published in Türkiye on national park between 2002 and 2021 within the scope of Clarivate Analytics' Web of Science (WoS). For this purpose, 156 publications, selected from the WoS database, were analyzed. The year 2017 had the most publications. In addition to Türkiye, significant countries in the spatial distribution analysis of national parks included the United States and Italy. About 338 authors in all were included in the author analysis for the publications from national parks. The current study found that 117 different institutions had publications about the national park. The highest number of publications and citations originated from Karadeniz Teknik and Kastamonu Universities, respectively. In this study, the WoS database was used as search engine because it is the most frequently preferred and recognized database, containing all records of high-quality research publications and continuing to be regarded as one of the primary sources of bibliographic information. However, some publications in other databases, such as Scopus and Google Scholar, cannot be overlooked in further research.
A Bibliometric Analysis of Scientific Research in Türkiye on National Parks Between 2002 and 2021
(2024.01.01) Yelsiz, M.S.; Yücedag, C.; Çiçek, N.; Ayan, S.
In Turkiye, national parks play a prominent role among protected areas. Besides meeting people's recreational needs, these protected areas are home to natural and cultural resources. As a result, numerous research has been conducted to find out more about assets and recreational uses of these areas. In this study, the bibliometric analysis method was used to examine research published in Turkiye on national park between 2002 and 2021 within the scope of Clarivate Analytics ' Web of Science (WoS). For this purpose, 156 publications, selected from the WoS database, were analyzed. The year 2017 had the most publications. In addition to T & uuml;rkiye, significant countries in the spatial distribution analysis of national parks included the United States and Italy. About 338 authors in all were included in the author analysis for the publications from national parks. The current study found that 117 different institutions had publications about the national park. The highest number of publications and citations originated from Karadeniz Teknik and Kastamonu Universities, respectively. In this study, the WoS database was used as search engine because it is the most frequently preferred and recognized database, containing all records of high-quality research publications and continuing to be regarded as one of the primary sources of bibliographic information. However, some publications in other databases, such as Scopus and Google Scholar, cannot be overlooked in further research.
A broad-spectrum biological activities of Heracleum humile extracts: A first report of the antiviral, anti-cancer and chemical properties
(2024.01.01) Ocal, M.; Yelken, S.D.; Altunoglu, Y.C.; Baloglu, M.C.; Soomro, S.I.; Zengin, G.; Angeloni, S.; Mustafa, A.M.; Caprioli, G.; Paksoy, M.Y.
The current work focused on researching the bioactive potential of ethyl acetate (EA), methanol (MeOH), and water extract of H. humile plant, specifically ethyl acetate, methanol extracts were acquired with maceration method, and water extract was obtained by the infusion method. The highest total phenolic ingredient was determined in the water extract, while the highest total flavonoid ingredient was found in the MeOH extract. The water extract displayed the most potent antioxidant activity in the assays used. The ethyl acetate extract showed the highest enzyme inhibition activity in Butyrylcholinesterase inhibition (BChE) and amylase assay. Antibacterial efficiency was analyzed using microdilution analysis. The minimum inhibitory concentration (MIC) amounts ranged from 125 to 500 mu g/mL except for water extract. The antiviral potential of the extracts was assessed against Bovine herpes virus 1 (BoHV-1) (Cooper strain). Methanol extract showed more potent antiviral effect. Selectivity indices were calculated for ethyl acetate and methanol extract as 3.32 and 13.39, respectively. All extracts displayed Deoxyribonucleic Acid (DNA) protection activity at approximately 25%-35%. For cell viability evaluation, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) test was performed against triple-negative human breast adenocarcinoma cells (MDA-MB-231) and Michigan Cancer Foundation-7 (MCF-7) cells. The most effective results were found in ethyl acetate extract at 48h. The 50% inhibitor concentrations (IC50) were determined for MDA-MB-231 and MCF-7 as 116.7 mu g/mL and 247.9 mu g/mL, respectively. Based on the bioactive compound content results, extracts of H. humile include valuable compounds that possess significant biological properties. In conclusion, these extracts could offer promising novel plant-derived therapeutics.
A Climate-Sensitive Approach for Determining the Urban Growth Boundaries: Towards a Spatial Exploration for Bursa, Türkiye
(2023.01.01) Isinkaralar, O.
Population growth is inevitable in urban areas responsible for climate crises worldwide, and urban development is affected by processes fed by many dynamics. Predicting and planning the growth limits of the city effectively is a critical issue for achieving sustainable urban growth and managing climate risks. The study used the cellular automata-Markov chain method to define development areas regarding natural structure and land use/land cover. It aimed to present a method that can be applied to different urban areas by focusing on effective urban growth management with a climate-sensitive approach. It offered a climate-sensitive approach to determining growth limits according to scenarios. The boundaries of 2030 have been determined for the city of Bursa, which exhibited an increased average summer value of the land surface temperature from 24 degrees C to 45 degrees C between 2012 and 2021, stands out with its natural riches, and shows a rapid urban growth trend. The proposed method modeled the limits of urban growth with a climate-sensitive approach, and the model's suitability was demonstrated by Kappa statistics (Klocation = 0.8884). The determined urban boundary will reduce the rate of the urban built-up area from 86% to 70% by 2030. While the climate crisis threatens all life, urban activities, urban growth, and sprawl continue to increase rapidly. However, the growth of urban areas within certain limits is a critical need for engineering and urban planning. For decision makers, spatially determining the limits of human intervention in nature is an important guideline. Extreme weather events and disasters such as floods, tsunamis, and changes in water levels brought about by climate change can potentially cause chaotic crises. Loss of life and economic crises may occur in possible processes. Therefore, measures must be taken on behalf of humanity and life to adapt to climate effects and keep the level of impact under control. Spatial models based on a wide range of temporal contexts have a structure that broadens our perspective. The study provides an approach that can be implemented in various fields with models produced based on scenarios.
A Climate-Sensitive Approach for Determining the Urban Growth Boundaries: Towards a Spatial Exploration for Bursa, Türkiye
(American Society of Civil Engineers (ASCE), 2023) Isinkaralar O.
Population growth is inevitable in urban areas responsible for climate crises worldwide, and urban development is affected by processes fed by many dynamics. Predicting and planning the growth limits of the city effectively is a critical issue for achieving sustainable urban growth and managing climate risks. The study used the cellular automata-Markov chain method to define development areas regarding natural structure and land use/land cover. It aimed to present a method that can be applied to different urban areas by focusing on effective urban growth management with a climate-sensitive approach. It offered a climate-sensitive approach to determining growth limits according to scenarios. The boundaries of 2030 have been determined for the city of Bursa, which exhibited an increased average summer value of the land surface temperature from 24°C to 45°C between 2012 and 2021, stands out with its natural riches, and shows a rapid urban growth trend. The proposed method modeled the limits of urban growth with a climate-sensitive approach, and the model's suitability was demonstrated by Kappa statistics (Klocation = 0.8884). The determined urban boundary will reduce the rate of the urban built-up area from 86% to 70% by 2030. © 2023 American Society of Civil Engineers.
A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation
(Springer, 2024) Ceylan, H.K.; Kırbay, F.Ö.; Yazgan, İ.; Elibol, M.
Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the circulating system are signs of pathology-associated diseases. Therefore, its precise and selective detection is critical for clinical applications to monitor the progression of the pathology. In this study, an optical immunoassay biosensor was developed as a model study for detecting recombinant VEGF165. The VEGF165 sample was purified from recombinant Kluyveromyces lactis GG799 yeast cells. Indirect ELISA was used during the detection, wherein iron oxide nanoparticles (FeNPs) were utilized to obtain optical signals. The FeNPs were synthesized in the presence of lactose p-amino benzoic acid (LpAB). VEGF165 antibody was conjugated to the LpAB-FeNPs through EDC/NHS chemistry to convert the iron oxide nanoparticles into VEGF165 specific probes. The specificity of the prepared system was tested in the presence of potential serum-based interferents (i.e., glucose, urea, insulin, C-reactive protein, and serum amyloid A), and validation studies were performed in a simulated serum sample. The proposed immunoassay showed a wide detection range (0.5 to 100 ng/mL) with a detection limit of 0.29 ng/mL. These results show that the developed assay could offer a sensitive, simple, specific, reliable, and high-throughput detection platform that can be used in the clinical diagnostics of VEGF. Graphical Abstract: (Figure presented.)
A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation
(2024.01.01) Ceylan, H.K.; Kirbay, F.O.; Yazgan, I.; Elibol, M.
Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the circulating system are signs of pathology-associated diseases. Therefore, its precise and selective detection is critical for clinical applications to monitor the progression of the pathology. In this study, an optical immunoassay biosensor was developed as a model study for detecting recombinant VEGF(165). The VEGF(165) sample was purified from recombinant Kluyveromyces lactis GG799 yeast cells. Indirect ELISA was used during the detection, wherein iron oxide nanoparticles (FeNPs) were utilized to obtain optical signals. The FeNPs were synthesized in the presence of lactose p-amino benzoic acid (LpAB). VEGF(165) antibody was conjugated to the LpAB-FeNPs through EDC/NHS chemistry to convert the iron oxide nanoparticles into VEGF(165) specific probes. The specificity of the prepared system was tested in the presence of potential serum-based interferents (i.e., glucose, urea, insulin, C-reactive protein, and serum amyloid A), and validation studies were performed in a simulated serum sample. The proposed immunoassay showed a wide detection range (0.5 to 100 ng/mL) with a detection limit of 0.29 ng/mL. These results show that the developed assay could offer a sensitive, simple, specific, reliable, and high-throughput detection platform that can be used in the clinical diagnostics of VEGF.
A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation
(2024) Ceylan, H.K.; Kırbay, F.Ö.; Yazgan, İ.; Elibol, M.
Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the circulating system are signs of pathology-associated diseases. Therefore, its precise and selective detection is critical for clinical applications to monitor the progression of the pathology. In this study, an optical immunoassay biosensor was developed as a model study for detecting recombinant VEGF. The VEGF sample was purified from recombinant Kluyveromyces lactis GG799 yeast cells. Indirect ELISA was used during the detection, wherein iron oxide nanoparticles (FeNPs) were utilized to obtain optical signals. The FeNPs were synthesized in the presence of lactose p-amino benzoic acid (LpAB). VEGF antibody was conjugated to the LpAB-FeNPs through EDC/NHS chemistry to convert the iron oxide nanoparticles into VEGF specific probes. The specificity of the prepared system was tested in the presence of potential serum-based interferents (i.e., glucose, urea, insulin, C-reactive protein, and serum amyloid A), and validation studies were performed in a simulated serum sample. The proposed immunoassay showed a wide detection range (0.5 to 100 ng/mL) with a detection limit of 0.29 ng/mL. These results show that the developed assay could offer a sensitive, simple, specific, reliable, and high-throughput detection platform that can be used in the clinical diagnostics of VEGF.
A Comparative Investigation of Arcobacter cryaerophilus Infection among Albino Crosses and High- and Low-Body-Weight Rainbow Trout
(Journal of Aquatic Animal Health 14:39 39-44, 2002, 2002) Aydın, Seyit; Engin, Murat; Bircan, Recep
Arcobacter cryaerophilus was isolated from naturally infected rainbow trout Oncorhynchus mykiss, and its pathogenicity was tested by intramuscular injection into healthy I-year-old high-body-weight (HBW) and low-body-weight (LB W) normally pigmented rainbow trout and albino crosses. Experimental infections caused deaths with gross clinical abnormalities such as exophthalmia, liver damage, bloody hemorrhagic kidney and heart, and swollen intestines. No significant differences in deaths were observed among the three infected fish groups. Hematocrit levels in blood of the experimentally infected HBW rainbow trout were significantly less than in healthy fish. No significant decreases were observed in the serum total protein of both the experimentally infected albino crosses and the high weight groups. Albumin and creatinine concentrations in serum were not significantly different among the three treatments.
A Comparative Study About Physical Properties of Copper Oxide and Zinc Oxide Nanoparticles on Fagus orientalis L. as Bioindicator
(Springer Nature, 2024) Isinkaralar, K.; Isinkaralar, O.; Özel, H.B.; Şevik, H.
Nanoparticles (NPs) have recently begun to be used extensively in many areas of our lives. It is stated that the resulting NPs pollution will affect even the most remote ecosystems on the Earth. Therefore, determining the effects of NPs on diverse ecosystems is a research topic of interest. From this perspective, current researches on NPs affecting forest ecosystems and forest trees are very scarce. The aim of this investigation was to reveal the inhibitory and toxic properties of CuO and ZnO-NPs on oriental beech seed germination parameters and identify the toxic threshold values of CuO and ZnO-NPs. Therefore, the oriental beech (Fagus orientalis L.) seeds obtained from ten populations (P1 to P10) were treated with CuO and ZnO-NPs as single compounds at concentrations of 200, 400, 600, 800 and 1000 mg/L and germination rate (GR), germination percentage (GP), root collar diameter (RCD), plumula length (PL), radicle thickness (RT) and radicle diameter (RD) were determined for 35 days. The water containing CuO-ZnO NPs as a single compound at 1000 mg/L negatively affected all growth in general. The significant decrease in our study occurred at P9. The CuO-ZnO NPs were reduced in GP, RCD, PL, RT, and RD as 3.5–4.2%; 11.2–0.1%; 9.1–36.4%; 23.07–38.46%; and 39.29–17.86%, while GR enhanced as 21.46–20.09%. It was found that water with a low concentration of NP (200 mg/L) is suitable for irrigation of seeds grown in soil media and does not have a significant toxic effect on the growth and uptake of metal ions. The findings and limitations of the present research allow us to assess the feasibility of reusing NP-contaminated water in agriculture. Nevertheless, further research is needed to understand the toxic effects of NP mixtures on growth and absorption mechanisms.
A Comparative Study About Physical Properties of Copper Oxide and Zinc Oxide Nanoparticles on Fagus orientalis L. as Bioindicator
(2024.01.01) Isinkaralar, K.; Isinkaralar, O.; Ozel, H.B.; Sevik, H.
Nanoparticles (NPs) have recently begun to be used extensively in many areas of our lives. It is stated that the resulting NPs pollution will affect even the most remote ecosystems on the Earth. Therefore, determining the effects of NPs on diverse ecosystems is a research topic of interest. From this perspective, current researches on NPs affecting forest ecosystems and forest trees are very scarce. The aim of this investigation was to reveal the inhibitory and toxic properties of CuO and ZnO-NPs on oriental beech seed germination parameters and identify the toxic threshold values of CuO and ZnO-NPs. Therefore, the oriental beech (Fagus orientalis L.) seeds obtained from ten populations (P1 to P10) were treated with CuO and ZnO-NPs as single compounds at concentrations of 200, 400, 600, 800 and 1000 mg/L and germination rate (GR), germination percentage (GP), root collar diameter (RCD), plumula length (PL), radicle thickness (RT) and radicle diameter (RD) were determined for 35 days. The water containing CuO-ZnO NPs as a single compound at 1000 mg/L negatively affected all growth in general. The significant decrease in our study occurred at P9. The CuO-ZnO NPs were reduced in GP, RCD, PL, RT, and RD as 3.5-4.2%; 11.2-0.1%; 9.1-36.4%; 23.07-38.46%; and 39.29-17.86%, while GR enhanced as 21.46-20.09%. It was found that water with a low concentration of NP (200 mg/L) is suitable for irrigation of seeds grown in soil media and does not have a significant toxic effect on the growth and uptake of metal ions. The findings and limitations of the present research allow us to assess the feasibility of reusing NP-contaminated water in agriculture. Nevertheless, further research is needed to understand the toxic effects of NP mixtures on growth and absorption mechanisms.
A comparison for thyroid functions and clini-cal features in deficit and non-deficit schizo-phrenia
(2024.01.01) Inaltekin, A.; Tasdelen, Y.
Objective: The primary occurrence and persistence of negative symptoms observed in patients with schizophrenia is deficit syndrome. Although the association between thyroid hormones and schizophrenia symptoms has been reported, no studies have investigated thyroid function in patients with deficit schizophrenia (DS). This study aims to investigate the clinical features and thyroid function in DS patients by comparing them with patients with non-deficit schizophrenia (NDS) and a control group. Method: 33 subjects from DS, 35 subjects from NDS and 35 healthy control subjects were included in the study. Patients with schizophrenia were classified into DS and NDS using the deficit syndrome table. Thyroid function was assessed by the levels of TSH, free T3 (fT3), and free T4 (fT4). Sociodemographic data and clinical characteristics were evaluated using the Sociodemographic Data Form, the Positive Symptoms Evaluation Scale (SAPS), the Negative Results: There was no significant difference between DS and NDS groups in terms of age, gender, marital status and education (p>0.05). The percentage of unemployed was significantly higher in the DS group than in the NDS group (p=0.005). There were 14 (42.4%) suicide attempts in the DS group and 11 (31.4%) in the NDS group, and there was no significant difference between the groups (p>0.05). There was no significant difference between the groups when comparing the thyroid functions of the DS, NDS and healthy control groups regarding fT4, fT3 and TSH (p>0.05). There was no significant correlation between TSH, free T3, free T4 and total SANS, total SAPS and CDSS scores (p>0.05). Discussion: According to our study thyroid function is not different in DS, NDS and healthy controls and is not associated with positive, negative and depressive symptoms in patients with schizophrenia.
A Comprehensive Overview of the Effects of Saffron Extract and Its Ingredients on Neurologic, Diabetes, Cardiovascular and Gastrointestinal Diseases: Biological Studies Using CRISPR-Cas9, Docking, Omics and Bioinformatics Approaches
(2023.01.01) Çeter, T.; Mollaamin, F.; Monajjemi, M.
Although the types of scientific tests used change over time, saffron intake has been found to reduce symptoms of diabetes, cholesterol, Alzheimer's, depression, and many other diseases. The purpose of this study was to provide safe and well-controlled clinical tests to clearly analyze the potential mechanisms of the effects of saffron. Biological markers after saffron use are measured in relationship with the predicted health results, so that the results of various studies can be compared and explicated. Since the natural propagation of saffron does not occur quickly and its corms can be manually dug up, separated and replanted, biotechnological methods can increase the ability to generate large amounts of various saffron compounds, such as crocin, picrocrocin, crocetin and safranal, in vitro. Since pathogenic plants cause crop losses in agriculture environment, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein 9 (Cas9) can help with biotic and abiotic problems via a crop molecular reproduction plan, with improvement of the genes' responses to issues induced through viruses, fungi, and bacteria. Quantitative Structure Activity Relationship (QSAR) methods can be used for determining the maintenance times of ingredients for saffron extract through analyzing solid phase micro-extraction gas chromatography-mass spectrometry (SPMEGC-MS). QSAR is an accurate method for detecting relationships between the molecular properties of chemical reactions and biological systems. Bioinformatics tools can help the mechanism of the transcriptome of saffron based on the structural foundation of the flavor, color biogenesis, genomic establishment and biological gynoecium of saffron. The data extracted from bioinformatics websites can be used for constructing biological routes containing the biosynthesis of main ingredients of saffron, i.e., crocin, crocetin, safranal, picrocrocin. Omics-based technologies have been extensively discussed within biology and can be used for saffron. These studies have given rise to concepts for the better understanding of saffron growth and its therapeutic action. Molecular docking simulation has also been discussed to find the location of safranal inside lysozyme. The interaction of safranal with molecular biology was discussed by mixing approaches including CRISPR/Cas9, docking, bioinformatics and omics. This provides a new approach that is in with the results obtained

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