Browsing by Author "Benek, Atakan"

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Antimicrobial Activity Screening of Ethanol Extract of and Investigation of Its Biochemical Composition.
(2019-01-10) Canlı, Kerem; Yetgin, Ali; Benek, Atakan; Bozyel, Mustafa Eray; Murat Altuner, Ergin
The aim of this study was to test antimicrobial activity of ethanol extract of against 22 bacteria and 1 yeast. Also, biochemical composition of the extract was investigated. A wide range of Gram-positive, Gram-negative microorganisms, and multidrug resistant bacteria were selected to test the antimicrobial activity. As a result, the extract is observed to contain fenchone )-) and camphor as major components and showed antimicrobial activity against all studied microorganisms except ATCC 25922 and . The results of the study present that is active against MDR strains too.
Bazı karayosunlarının antimikrobiyal, antioksidan ve antibiyofilm aktivitelerinin belirlenmesi
(Kastamonu Üniversitesi, 2020) Benek, Atakan
İnsanlar tarih boyunca çeşitli yöntemleri ve ilaçları kullanarak hastalıklar ile mücadele etmiştir. Yüzyıllar boyunca hastalıklara karşı sürdürülen bu mücadelede kullanılan ilaçların hammaddelerini çoğunlukla doğal ürünler oluşturmuştur, fakat gelişen teknoloji ile artan ilaç talebi sentetik ilaç üretiminin doğmasını ve yaygınlaşmasını sağlamıştır. Günümüzde hastalıklara karşı kullanılmakta olan sentetik ilaçların yetersiz kalmaya başlaması, bakterilerin antibiyotiklere karşı dirençlerinin artması nedeniyle antibiyotiklerin kullanılabildikleri sürenin oldukça azalması ve aynı zamanda kullanılmakta olan ilaçların yan etkilerinin tespit edilmesi, daha düşük yan etki spektrumuna sahip doğal ürünlerin tespit edilme zorunluluğunu arttırmıştır. Sentetik ilaçların yetersiz kalması ile ortaya çıkan durum sonucunda dünya genelinde bilim insanları tarih boyunca geleneksel tıpta kullanılan ve doğada bulunan farklı bitkilerin farmakolojik aktiviteleri üzerine araştırmalar yapmaya başlamıştır ve araştırılan bitki gruplarından birisi de karayosunlarıdır. Çinli ve Amerika yerlileri karayosunlarının yaraların iyileşmesinde etkili olduğunu ve yaralardaki enfeksiyonları azalttığını yüzyıllar önce keşfetmişlerdir. Bu keşiften itibaren günümüze kadar karayosunları hakkında pek çok araştırma yapılmış ve çeşitli alanlarda kullanılmıştır. Karayosunları farklı sistematik ekollere göre değişmek ile birlikte, 1 036 cins ve yaklaşık 18 409 türe sahip Bryophyta divizyosu içerisinde bulunmaktadır. Çok sayıda türe sahip olan karayosunları gösterdikleri güçlü farmakolojik etkileri basit yapıları ve kültüre alınabilme potansiyelleri ile günümüzde ilaçların içinde bulunduğu yetersiz durumdan çıkış için umut vaat etmektedir. Bu tez çalışmasında 6 farklı karayosunu örneğinin 20 farklı suş üzerinde antimikrobiyal etkisi ve DPPH yöntemi ile antioksidan kapasitesi in vitro olarak incelenmiştir. Ayrıca karayosunu örneklerinin Listeria innocua suşuna karşı antibiyofilm aktiviteleri belirlenmiştir.
Biochemical and Antioxidant Properties as well as Antimicrobial and Antibiofilm Activities of subsp. (Vved.) Stearn.
(2023-06-06T21:00:00Z) Canli, Kerem; Turu, Dilay; Benek, Atakan; Bozyel, Mustafa Eray; Simsek, Özcan; Altuner, Ergin Murat
In this study, the chemical composition and biological activity of subsp. (Vved.) Stearn were investigated for the first time, focusing on its antimicrobial, antioxidant, and antibiofilm properties. A GC-MS analysis was employed to evaluate the composition of its secondary metabolites, identifying linoleic acid, palmitic acid, and octadecanoic acid 2,3-dihydroxypropyl ester as the major compounds in ethanol extract. The antimicrobial activity of subsp. was assessed against 26 strains, including standard, food isolate, clinical isolate, and multidrug-resistant ones, as well as three species using the disc diffusion method and the determination of the minimum inhibitory concentration (MIC). The extract showed strong antimicrobial activity against strains, including methicillin-resistant and multidrug-resistant strains, as well as and . Its antioxidant capacity was evaluated using the DPPH method, revealing a high level of antioxidant activity in the plant. Additionally, the antibiofilm activity of subsp. was determined, demonstrating a reduction in biofilm formation for the ATCC 25922 strain and an increase in biofilm formation for the other tested strains. The findings suggest potential applications of subsp. in the development of novel antimicrobial, antioxidant, and antibiofilm agents.
Biochemical, Antioxidant Properties and Antimicrobial Activity of Epiphytic Leafy Liverwort (L.) Dumort.
(2023-05-04T00:00:00Z) Simsek, Ozcan; Canli, Kerem; Benek, Atakan; Turu, Dilay; Altuner, Ergin Murat
In this study, the biochemical, antioxidant properties, and antimicrobial activity of the epiphytic leafy liverwort (L.) Dumort were investigated. Due to the scarcity and difficulty in obtaining liverworts, research on their bioactivity is limited; thus, this study aimed to uncover the potential of . The antimicrobial activity was evaluated against various microorganisms, including food isolates, clinical isolates, multidrug-resistant strains, and standard strains, using the disk diffusion method and determining the minimum inhibitory concentration (MIC) values. This study represents the first antioxidant investigation on and an antimicrobial study using ethanol extract and the disk diffusion method. Notably, susceptibility was observed in ATCC 29212, FI, ATCC 7644, MDR, and ATCC 25923. The antioxidant capacity was assessed using the DPPH method, emphasizing the high scavenging performance. Gas chromatography-mass spectrometry (GC-MS) analysis identified the primary compounds as frullanolide (19.08%), 2,3-Dimethylanisole (15.21%), linoleic acid (11.11%), palmitic acid (9.83%), and valerenic acid (5.3%). The results demonstrated the significant antimicrobial activity of against the tested microorganisms and its potent antioxidant properties. These findings emphasize the potential of as a promising source of natural antimicrobial and antioxidant agents, underscoring the importance of further investigation into its bioactive compounds and elucidating the mechanisms of action in future studies.
Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic .
(2023-08-02) Canli, Kerem; Bozyel, Mustafa Eray; Turu, Dilay; Benek, Atakan; Simsek, Ozcan; Altuner, Ergin Murat
(Lamiaceae) is an Eastern Mediterranean plant that is widely used in Turkish traditional medicine. This study aimed to investigate the biochemical composition, antimicrobial activity, and antioxidant potential of . In this study, the biochemical composition of the ethanol extract (OOEt) was analyzed using GC-MS. The antimicrobial activity was investigated using a disk diffusion test and determining minimum inhibitory concentrations (MIC) against 30 microorganism strains, including 28 bacteria (some multidrug-resistant) and 2 fungi. Additionally, the antioxidant activity was evaluated using the DPPH method. The main component identified was carvacrol. OOEt demonstrated antimicrobial activity against a wide range of tested microorganism strains. OOEt displayed the highest activity against (a Gram-positive bacterium) at 100 µL with a 52 mm inhibition zone. Additionally, DSMZ 50071 and P1, which are Gram-negative bacteria, were the most sensitive strains with a 24 mm inhibition zone in 100 µL of OOEt. The data obtained from (a multidrug-resistant strain) is particularly striking, as higher activity was observed compared to all positive controls. All tested fungal strains showed more effective results than positive controls. The antioxidant activity of OOEt was found to be stronger than that of the positive control, ascorbic acid. This study determined that has significant antimicrobial and antioxidant potential.
Biological Activities and Biochemical Composition of Endemic .
(2023-04-09T00:00:00Z) Tunca-Pinarli, Yagmur; Benek, Atakan; Turu, Dilay; Bozyel, Mustafa Eray; Canli, Kerem; Altuner, Ergin Murat
In this study, we investigated the antimicrobial, antioxidant, and antibiofilm activities and the biochemical composition of . The antimicrobial activity of ethanol extract (AFEt) was tested against 48 strains, and this is the first study testing the antimicrobial activity of this plant to this extent. The antioxidant activity was determined using the DPPH assay, and the antibiofilm activity of aqueous extract (AFAq) against five strains was assessed. The chemical composition of the plant extract was determined using GC-MS with artemisia ketone (19.41%) as the main component. The findings indicated that AFEt displayed antimicrobial activity against 38 strains, with a particular efficacy observed against various strains, such as ATCC 25923, clinically isolated, multidrug resistant (MDR), and methicillin-resistant (MRSA) strains. In addition, the highest activity was observed against . Moreover, the extract demonstrated activity against strains. The plant extract also showed relatively good antioxidant activity compared to ascorbic acid, with an value of 55.52 µg/mL. However, AFAq acted as a biofilm activator against ATCC 25922, increasing the biofilm formation by 2.63-fold. In conclusion, our study demonstrates the potential of as a source of antimicrobial and antioxidant agents.