Browsing by Author "Altuner E., Çeter T., Alpas H."

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Effect of high hydrostatic pressure on the profile of proteins extracted from Betula pendula pollens
(2014-10-02) Altuner E., Çeter T., Alpas H.; Altuner, EM, Ceter, T, Alpas, H
High hydrostatic pressure (HHP) has high success potential in pollen protein extraction, but its effect on pollen protein profiles has not been studied yet. The aim of this study is to put forward whether HHP processing causes a change in the protein profiles extracted from pollens or not. In this study, proteins extracted from Betula pendula pollens were studied at 100, 200 and 300MPa at room temperature for 5min. In addition, the efficiency of three different extraction solvents, namely phosphate buffer saline (PBS) buffer pH 7.5, trichloroacetic acid-acetone and Tris-HCl buffer pH 8.8, was also observed, and the results were compared with the conventional pollen protein extraction procedure. As a result, it is concluded that 200MPa for 5min has extracted similar amounts of protein compared with the conventional extraction method which lasted for 24h, which lasted for 24 h. On the other hand, the application time for 200 MPa for 5 min is extremely shorter when it is compared to the conventional extraction method.
High hydrostatic pressure processing: A method having high success potential in pollen protein extraction
(2012-06-01) Altuner E., Çeter T., Alpas H.; Altuner, EM, Ceter, T, Alpas, H
Even a single peptide that is present in the pollen wall and cytoplasm could cause pollen allergy. To produce skin-prick test kits, the first step is the extraction of these molecules. In this study, Cedrus atlantica pollens were subjected to 220 and 330MPa for 10 and 30min in order to extract these molecules. After high hydrostatic pressure processing (HHPP), the total amounts of proteins (TAPs) are measured and compared with the results of the conventional extraction method (CEM). As a result, the TAPs extracted by HHPP is 18.0210 μg/mL at 220MPa for 10min, 22.5770 μg/mL at 220MPa for 30min, 23.3810 μg/mL at 330MPa for 10min and 25.9270 μg/mL at 330MPa for 30min, while this is 1.9460 μg/mL in 24h by the CEM. In addition to these results, visual pollen deformation and eruption, pollen wall and surface damage have also been observed. © 2012 Taylor & Francis.