Browsing by Author "Altuner, Ergin Murat"
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Pubmed ANTIMICROBIAL SCREENING OF ROOTS.(2016-08-12) Canlı, Kerem; Yetgin, Ali; Akata, Ilgaz; Altuner, Ergin MuratIt was previously shown that some parts of , which is commonly known as oud or oodh, such as roots have been used as a traditional medical herbal in different countries. In Turkey is one of the ingredients while preparing famous Mesir paste, which was invented as a medicinal paste and used from the Ottoman period to now at least for 500 years. The identification the antimicrobial activity of ethanol extract of roots is main purpose of this analysis.Pubmed Biochemical and Antioxidant Properties as well as Antimicrobial and Antibiofilm Activities of subsp. (Vved.) Stearn.(2023-06-06T21:00:00Z) Canli, Kerem; Turu, Dilay; Benek, Atakan; Bozyel, Mustafa Eray; Simsek, Özcan; Altuner, Ergin MuratIn this study, the chemical composition and biological activity of subsp. (Vved.) Stearn were investigated for the first time, focusing on its antimicrobial, antioxidant, and antibiofilm properties. A GC-MS analysis was employed to evaluate the composition of its secondary metabolites, identifying linoleic acid, palmitic acid, and octadecanoic acid 2,3-dihydroxypropyl ester as the major compounds in ethanol extract. The antimicrobial activity of subsp. was assessed against 26 strains, including standard, food isolate, clinical isolate, and multidrug-resistant ones, as well as three species using the disc diffusion method and the determination of the minimum inhibitory concentration (MIC). The extract showed strong antimicrobial activity against strains, including methicillin-resistant and multidrug-resistant strains, as well as and . Its antioxidant capacity was evaluated using the DPPH method, revealing a high level of antioxidant activity in the plant. Additionally, the antibiofilm activity of subsp. was determined, demonstrating a reduction in biofilm formation for the ATCC 25922 strain and an increase in biofilm formation for the other tested strains. The findings suggest potential applications of subsp. in the development of novel antimicrobial, antioxidant, and antibiofilm agents.Pubmed Biochemical, Antioxidant Properties and Antimicrobial Activity of Epiphytic Leafy Liverwort (L.) Dumort.(2023-05-04T00:00:00Z) Simsek, Ozcan; Canli, Kerem; Benek, Atakan; Turu, Dilay; Altuner, Ergin MuratIn this study, the biochemical, antioxidant properties, and antimicrobial activity of the epiphytic leafy liverwort (L.) Dumort were investigated. Due to the scarcity and difficulty in obtaining liverworts, research on their bioactivity is limited; thus, this study aimed to uncover the potential of . The antimicrobial activity was evaluated against various microorganisms, including food isolates, clinical isolates, multidrug-resistant strains, and standard strains, using the disk diffusion method and determining the minimum inhibitory concentration (MIC) values. This study represents the first antioxidant investigation on and an antimicrobial study using ethanol extract and the disk diffusion method. Notably, susceptibility was observed in ATCC 29212, FI, ATCC 7644, MDR, and ATCC 25923. The antioxidant capacity was assessed using the DPPH method, emphasizing the high scavenging performance. Gas chromatography-mass spectrometry (GC-MS) analysis identified the primary compounds as frullanolide (19.08%), 2,3-Dimethylanisole (15.21%), linoleic acid (11.11%), palmitic acid (9.83%), and valerenic acid (5.3%). The results demonstrated the significant antimicrobial activity of against the tested microorganisms and its potent antioxidant properties. These findings emphasize the potential of as a promising source of natural antimicrobial and antioxidant agents, underscoring the importance of further investigation into its bioactive compounds and elucidating the mechanisms of action in future studies.Pubmed Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic .(2023-08-02) Canli, Kerem; Bozyel, Mustafa Eray; Turu, Dilay; Benek, Atakan; Simsek, Ozcan; Altuner, Ergin Murat(Lamiaceae) is an Eastern Mediterranean plant that is widely used in Turkish traditional medicine. This study aimed to investigate the biochemical composition, antimicrobial activity, and antioxidant potential of . In this study, the biochemical composition of the ethanol extract (OOEt) was analyzed using GC-MS. The antimicrobial activity was investigated using a disk diffusion test and determining minimum inhibitory concentrations (MIC) against 30 microorganism strains, including 28 bacteria (some multidrug-resistant) and 2 fungi. Additionally, the antioxidant activity was evaluated using the DPPH method. The main component identified was carvacrol. OOEt demonstrated antimicrobial activity against a wide range of tested microorganism strains. OOEt displayed the highest activity against (a Gram-positive bacterium) at 100 µL with a 52 mm inhibition zone. Additionally, DSMZ 50071 and P1, which are Gram-negative bacteria, were the most sensitive strains with a 24 mm inhibition zone in 100 µL of OOEt. The data obtained from (a multidrug-resistant strain) is particularly striking, as higher activity was observed compared to all positive controls. All tested fungal strains showed more effective results than positive controls. The antioxidant activity of OOEt was found to be stronger than that of the positive control, ascorbic acid. This study determined that has significant antimicrobial and antioxidant potential.Pubmed Biological Activities and Biochemical Composition of Endemic .(2023-04-09T00:00:00Z) Tunca-Pinarli, Yagmur; Benek, Atakan; Turu, Dilay; Bozyel, Mustafa Eray; Canli, Kerem; Altuner, Ergin MuratIn this study, we investigated the antimicrobial, antioxidant, and antibiofilm activities and the biochemical composition of . The antimicrobial activity of ethanol extract (AFEt) was tested against 48 strains, and this is the first study testing the antimicrobial activity of this plant to this extent. The antioxidant activity was determined using the DPPH assay, and the antibiofilm activity of aqueous extract (AFAq) against five strains was assessed. The chemical composition of the plant extract was determined using GC-MS with artemisia ketone (19.41%) as the main component. The findings indicated that AFEt displayed antimicrobial activity against 38 strains, with a particular efficacy observed against various strains, such as ATCC 25923, clinically isolated, multidrug resistant (MDR), and methicillin-resistant (MRSA) strains. In addition, the highest activity was observed against . Moreover, the extract demonstrated activity against strains. The plant extract also showed relatively good antioxidant activity compared to ascorbic acid, with an value of 55.52 µg/mL. However, AFAq acted as a biofilm activator against ATCC 25922, increasing the biofilm formation by 2.63-fold. In conclusion, our study demonstrates the potential of as a source of antimicrobial and antioxidant agents.Pubmed Comparison of Antimicrobial Activity of Cloves from China and Taşköprü, Turkey.(2018-11-27) Yetgin, Ali; Canlı, Kerem; Altuner, Ergin MuratIn this study, antimicrobial activities of two different samples of L. from Turkey (TR) (Taşköprü, Kastamonu, Turkey) and China (CN) were determined. A broad spectrum of Gram-negative and Gram-positive bacteria (17 bacteria) including species of , , , , , , , , and were used for testing antibacterial activity. In addition, antifungal activity against was also investigated. Antimicrobial activity was tested by using 3 different processes (chopping, freezing, and slicing by the disk diffusion method). The results showed that TR garlic presented more antimicrobial activity than CN garlic. Mechanism of activity of CN garlic could be proposed to be different from that of TR garlic.Pubmed IN VITRO ANTIMICROBIAL ACTIVITY SCREENING OF XYLARIA HYPOXYLON(2016-07-03) Canli, Kerem; Akata, Ilgaz; Altuner, Ergin MuratFungi have a potential of using both as nutritive and medicinal food stuff. Because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. The aim of this study is to determine the antimicrobial activity of , which were collected from Yomra, Trabzon, Turkey.Pubmed [Investigation of antifungal activity of Ononis spinosa L. ash used for the therapy of skin infections as folk remedies].(2010-10-01T00:00:00Z) Altuner, Ergin Murat; Ceter, Talip; Işlek, CemilTraditional folk remedies used for centuries come up focus of interest in recent years, due to the trend of use of herb-derived natural products. In addition, increasing morbidity and mortality rates of opportunistic fungal infections and accelerating antifungal resistance rates of fungi lead to the use of alternative therapies with herb-derived preparations as novel antifungals. Ononis spinosa L. (spiny restharrow), which is classified in Leguminosae family, is one of the plants used in herbal medicine as folk remedies for the treatment of skin lesions and/or infections as well as many other disorders. Antibacterial, antifungal, anti-inflammatory and analgesic effects of Ononis spinosa (OS) have already been supported by different studies. The roots and aerial sections of OS are the mainly employed parts for application, however local communities inhabiting at southeastern parts of Anatolia, Turkey, employ the ashes of OS widely to heal the skin infections. There have been no reports about the antifungal activity of OS ashes as far as the current literature is concerned. The aim of this study was to investigate the antifungal activity of ashes of OS, collected from a rural area located at Southeast Anatolia. Ashes of OS have been obtained by burning the plant samples at 400°C, and extracted in sterile distilled water and ethanol. The efficacy of aqueous and ethanol extracts of OS ashes were tested against 10 fungi, of which one was a Candida albicans standard strain (ATCC 95071) and the others were clinical isolates (C.albicans, Candida glabrata, Candida tropicalis, Candida krusei, Candida guilliermondii, Candida parapsilosis, Candida pelliculosa, Trichosporon asahii, Trichophyton rubrum). Antifungal susceptibility test was performed by disc diffusion (DD) method and the results were confirmed with minimum inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values determined by microdilution method. The results indicated that both aqueous and ethanol extracts of OS ash showed antifungal activity against C. Albicans ATCC 95071 (DD inhibition zones were 16 and 15 mm, respectively; MIC = 1.25 µg/ml, MFC = 1.25 µg/ml), whereas against C.glabrata clinical isolate only ethanol extract exhibited antifungal activity (DD inhibition zone = 10 mm, MIC = 5.00 µg/ml, MFC = 40.00 µg/ml). No antifungal effect was detected against the other clinical Candida spp, T.asahii and T.rubrum isolates. In conclusion, since our results emphasize that extracts of OS ash that traditionally used for skin disorders, showed promising degrees of antifungal activity against some of Candida strains, these preliminary data should be supported by further large-scale studies.Pubmed Some Bryophytes Trigger Cytotoxicity of Stem Cell-like Population in 5-Fluorouracil Resistant Colon Cancer Cells.(2021-06-20) Özerkan, Dilşad; Erol, Ayşe; Altuner, Ergin Murat; Canlı, Kerem; Kuruca, Dürdane SerapColorectal cancer is the third most common cancer worldwide. Cancer stem cells are known to play an important role in relapse, and metastases of the disease after chemotherapy. Investigation of new drugs, and their combinations targeting these cells and thus eliminating cancer is one of the most urgent needs of today's chemotherapy. The aim of the present study was to evaluate the effects of Bryophytes like (AA), (HS), (TT), (SR), (BR) species extracted with ethyl alcohol on 5-fluorouracil(5-FU) resistant colorectal cancer cell lines (HCT116 and HT29). After extraction, stock solutions of bryophytes were prepared, and IC values were detected in drug-resistant cells obtained with 5-FU application. CD24+, CD44+/CD133+ surface markers and P-glycoprotein (P-gp) mediated efflux were isolated from both 5-FU treated cells and analyzed using the flow cytometry. In all bryophyte-treated groups, the binding Rho123 (low Rho fluorescence) and Rho (high Rho fluorescence) were sorted from 5-FU resistant HCT116, and HT-29 cells. All types of bryophytes were found cytotoxic. Bryophyte extract reduced the percentage of Rho cells in cultures incubated with 5-FU. In summary, the implementation of these bryophytes might be regarded as an effective approach for treatment of colorectal cancer due to their cytotoxic effect that decreases the recurrence of the disease.Supplemental data for this article is available online at https://doi.org/10.1080/01635581.2021.1933098.
