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Presence of quorum sensing system, virulence genes, biofilm formation and relationship among them and class 1 integron in carbapenem-resistant clinical Pseudomonas aeruginosa isolates

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dc.contributor.authorBaşkan, Ceren
dc.contributor.authorSırıken, Belgin
dc.contributor.authorTüfekci, Enis Fuat
dc.contributor.authorKılınç, Çetin
dc.contributor.authorErtürk, Ömer
dc.contributor.authorErol, İrfan
dc.date.accessioned2026-01-04T17:02:22Z
dc.date.issued2022-07-08
dc.description.abstractCarbapenems are the most effective agents for treating clinical P. aeruginosa (PsA) infections. During an infection, a quorum-sensing (QS) system and its regulating virulence genes have a great role. The aim of the study was to detect the presence of a las and rhl QS system and related virulence genes, biofilm formation and a class 1 (Cls1) integron. A total of 52 carbapenem-resistant PsA (CRPsA) isolates obtained from Kastamonu, Turkey was analyzed. For the isolation and identification of CRPsA isolates, a conventional culture method, an automated VITEK-2 compact system, and oprL gene-based molecular technique were applied. The two QS system genes were detected in 51 (98.1%), and co-existed of four two QS system genes (lasI/R and rhIl/R genes) were determined in 41 (78.8%) of the isolates. algD, lasB, toxA and aprA genes were detected in between 46.1 and 88.5%, and co-existence of four two QS system genes with four virulence genes were detected in 40.4% of the isolates. Biofilm formation using microtiter plate assay and slime production using Congo Red Agar and Cls1 integron were determined in 84.6%, 67.3% and 51.9% of the isolates, respectively. According to statistical analyses results, there was a significant positive correlation (p < .10) between the las and the rhl systems and a strongly and positive correlation (p < .01 or p < .05) between the rhl system-three virulence genes and slime production-and among some virulence genes. In conclusion, the CRPsA isolates tested in the study are highly virulent and QS systems have a significant role in pathogenesis.
dc.description.urihttps://doi.org/10.1007/s00203-022-03061-y
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/35802194
dc.identifier.doi10.1007/s00203-022-03061-y
dc.identifier.eissn1432-072X
dc.identifier.issn0302-8933
dc.identifier.openairedoi_dedup___::8eef8dd0dc2003aa52e88ba24a30fc07
dc.identifier.orcid0000-0001-7849-4459
dc.identifier.orcid0000-0001-5051-2694
dc.identifier.pubmed35802194
dc.identifier.scopus2-s2.0-85133729665
dc.identifier.urihttps://hdl.handle.net/20.500.12597/39852
dc.identifier.volume204
dc.identifier.wos000823624200001
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofArchives of Microbiology
dc.rightsCLOSED
dc.subjectBacterial Proteins
dc.subjectCarbapenems
dc.subjectVirulence
dc.subjectVirulence Factors
dc.subjectBiofilms
dc.subjectDrug Resistance, Bacterial
dc.subjectPseudomonas aeruginosa
dc.subjectQuorum Sensing
dc.subjectIntegrons
dc.subject.sdg3. Good health
dc.titlePresence of quorum sensing system, virulence genes, biofilm formation and relationship among them and class 1 integron in carbapenem-resistant clinical Pseudomonas aeruginosa isolates
dc.typeArticle
dspace.entity.typePublication
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