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Therapeutic potential of young plasma in reversing age-related liver inflammation via modulation of NLRP3 inflammasome and necroptosis

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dc.contributor.authorBaba, Burcu
dc.contributor.authorCeylani, Taha
dc.contributor.authorTeker, Hikmet Taner
dc.contributor.authorKeskin, Seda
dc.contributor.authorGenc, Aysun Inan
dc.contributor.authorGurbanov, Rafig
dc.contributor.authorAcikgoz, Eda
dc.date.accessioned2026-01-04T21:38:10Z
dc.date.issued2025-01-01
dc.description.abstractAbstract The phenomenon of inflammaging, characterized by an increase in low-grade chronic inflammation, is closely associated with diseases related to liver dysfunction. This study investigated daily plasma exchange between 5-week-old and 24-month-old Sprague Dawley rats for 30 days, focusing on protein secondary structures, NLRP3 inflammasome, and necroptosis. Conformation changes in protein secondary structures were identified by infrared spectroscopy-based pattern recognition analysis. Liver biopsies with histochemical and immunohistochemical staining were used to assess molecules associated with inflammation, necroptosis and NLRP3 inflammasome complex. Expression levels of NLRP3 components were determined by qPCR. Enhanced random coils, 310 helices, β-turns, and loop structures were identified in old rats and young rats with old plasma. Young rats and old rats with young plasma displayed higher α-helices and β-sheet structures. Young rats with old plasma showed increased NLRP3, ASC, caspase-1, IL-1β, and IL-18 mRNA levels, indicating an inflammatory response. Whereas old rats with young plasma exhibited lower inflammation levels. Histological evaluations revealed that young rats receiving aged plasma showed significantly increased levels of NLRP3, ASC, caspase-1, IL-1β, TNF-α, VEGFR2, RIPK1, and MLKL immunoreactivity, whereas decreased immunoreactivity in aged rats receiving young plasma. These findings suggest that young plasma reduces NLRP3 inflammasome activation and necroptosis in aged rats.
dc.description.urihttps://doi.org/10.1007/s10522-025-10260-9
dc.description.urihttps://doi.org/10.2139/ssrn.5133035
dc.description.urihttp://dx.doi.org/10.1007/s10522-025-10260-9
dc.description.urihttps://avesis.yyu.edu.tr/publication/details/e059b3e3-b4e7-4319-aaf8-961ceae61b76/oai
dc.description.urihttps://hdl.handle.net/20.500.12639/7540
dc.identifier.doi10.1007/s10522-025-10260-9
dc.identifier.eissn1573-6768
dc.identifier.issn1389-5729
dc.identifier.openairedoi_dedup___::b54a7f18b943a9b6a8fc95d034c88772
dc.identifier.orcid0000-0002-3041-6010
dc.identifier.pubmed40418410
dc.identifier.scopus2-s2.0-105006463539
dc.identifier.urihttps://hdl.handle.net/20.500.12597/42429
dc.identifier.volume26
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofBiogerontology
dc.rightsOPEN
dc.subjectNecroptosis
dc.subjectPlasma exchange
dc.subjectNLRP3 Inflammasome
dc.subjectInflammaging
dc.subjectInfrared spectroscopy
dc.subjectResearch Article
dc.titleTherapeutic potential of young plasma in reversing age-related liver inflammation via modulation of NLRP3 inflammasome and necroptosis
dc.typeArticle
dspace.entity.typePublication
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