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Effect of serotonin 7 receptors and transcript variants on RIN-5F pancreatic beta cell line

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dc.contributor.authorYayla, Muhammed
dc.contributor.authorToktay, Erdem
dc.contributor.authorOzdemir Sarikaya, Bengul
dc.contributor.authorErmis, Ugur
dc.contributor.authorAkgun, Sakir
dc.contributor.authorCinar, Irfan
dc.date.accessioned2026-01-04T22:02:30Z
dc.date.issued2025-06-01
dc.description.abstractThe physiological effects of 5-HT7 receptors expressed in pancreatic beta cells have not yet been elucidated. We first aimed to investigate the effect of 5-HT7 receptor agonist (AS19) and antagonist (SB269970) application on insulin secretion in RIN-5F pancreatic beta cells. Subsequently, we aimed to investigate the effects of agonist and antagonist applications on cell damage induced by STZ.Cell damage was caused by giving 5 mM STZ solution to the cells for 12 h. The protective effects of 5-HT7 receptor agonist and antagonist on this subsequent damage were investigated. IGF-1, TNF-α, TGF-B1, NF-KB, Bax, Caspase 3, Caspase 9, 5-HT7, 5HT7x2, 5HT7x3 mRNA expression levels were compared between groups.While agonist application stimulates insulin secretion, the effect of the antagonist varies.SB269970 reduced oxidative stress and downregulated TNF-a, TGF-B1 and NF-KB expression and also prevented apoptosis by decreasing Bax, caspase 3 and 9 levels against STZ-induced beta cell damage.The effect of 5-HT7 receptors on insulin secretion and their effects against STZ damage will be guiding for more detailed studies in the treatment of diabetes and related diseases in the future.
dc.description.urihttps://doi.org/10.1016/j.tiv.2025.106050
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/40086649
dc.identifier.doi10.1016/j.tiv.2025.106050
dc.identifier.issn0887-2333
dc.identifier.openairedoi_dedup___::d0c280d5b6505fcec458f30cb2a2d817
dc.identifier.pubmed40086649
dc.identifier.scopus2-s2.0-86000539118
dc.identifier.startpage106050
dc.identifier.urihttps://hdl.handle.net/20.500.12597/42702
dc.identifier.volume106
dc.language.isoeng
dc.publisherElsevier BV
dc.relation.ispartofToxicology in Vitro
dc.rightsCLOSED
dc.subjectSulfonamides
dc.subjectApoptosis
dc.subjectStreptozocin
dc.subjectRats
dc.subjectCell Line
dc.subjectSerotonin Receptor Agonists
dc.subjectOxidative Stress
dc.subjectInsulin-Secreting Cells
dc.subjectReceptors, Serotonin
dc.subjectInsulin Secretion
dc.subjectAnimals
dc.subjectInsulin
dc.subjectSerotonin Antagonists
dc.subjectRNA, Messenger
dc.titleEffect of serotonin 7 receptors and transcript variants on RIN-5F pancreatic beta cell line
dc.typeArticle
dspace.entity.typePublication
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