The effect of N-acetylcysteine use against ER stress in kidney tissue of obese rats

Abstract

Endoplasmic reticulum (ER) stress has recently gained increasing importance in the pathogenesis of obesity-associated kidney disease. N-acetylcysteine (NAC) is an antioxidant that acts directly and indirectly through upregulating cellular antioxidants. This study's obesity model was created using a high-fat (60% k/cal) diet. NAC (150 mg/kg) was administered by intragastric gavage for eight weeks in both the NAC and obesity + NAC (ObNAC) groups. GRP78 and PERK expressions were determined immunohistochemically in sections taken from kidney tissues obtained at the end of the experiment. The H score of GRP78 was significantly higher in the Ob group than in the Cont, NAC, and ObNAC groups (p 0.05). The PERK H score of the Ob group was also significantly higher than the Cont, NAC, and ObNAC groups (p