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Lipidomic markers of sperm cryotolerance in cattle

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dc.contributor.authorEvans, Holly C.
dc.contributor.authorDinh, Thu T. N.
dc.contributor.authorUgur, Muhammet Rasit
dc.contributor.authorHitit, Mustafa
dc.contributor.authorSajeev, Dishnu
dc.contributor.authorKaya, Abdullah
dc.contributor.authorTopper, Einko
dc.contributor.authorNicodemus, Molly C.
dc.contributor.authorSmith, Gary D.
dc.contributor.authorMemili, Erdogan
dc.date.accessioned2026-01-04T14:41:27Z
dc.date.issued2020-11-19
dc.description.abstractAbstractThe objective of the current study was to determine the fatty acid composition of sperm from Holstein bulls with different freezability (Good and Poor;n= 12). Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform–methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography–mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (P< 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18 µg) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9 µg;P< 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P≤ 0.011). These results are significant because they reveal key understandings of fatty acid composition of sperm membrane and lay a foundation for the manipulation of membrane integrity, fluidity, and stability to advance the assisted reproductive technologies.
dc.description.urihttps://doi.org/10.1038/s41598-020-77089-9
dc.description.urihttps://www.nature.com/articles/s41598-020-77089-9.pdf
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/33214639
dc.description.urihttp://dx.doi.org/10.1038/s41598-020-77089-9
dc.description.urihttps://dx.doi.org/10.1038/s41598-020-77089-9
dc.description.urihttps://aperta.ulakbim.gov.tr/record/3383
dc.identifier.doi10.1038/s41598-020-77089-9
dc.identifier.eissn2045-2322
dc.identifier.openairedoi_dedup___::05e655cf8859dff43d5fbe1cb211835b
dc.identifier.orcid0000-0003-1234-6693
dc.identifier.pubmed33214639
dc.identifier.scopus2-s2.0-85096308777
dc.identifier.urihttps://hdl.handle.net/20.500.12597/38348
dc.identifier.volume10
dc.identifier.wos000594637300006
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofScientific Reports
dc.rightsOPEN
dc.subjectCryopreservation
dc.subjectMale
dc.subjectFatty Acids
dc.subjectLipids
dc.subjectSpermatozoa
dc.subjectArticle
dc.subjectGas Chromatography-Mass Spectrometry
dc.subjectLipidomics
dc.subjectAnimals
dc.subjectCattle
dc.subjectSemen Preservation
dc.titleLipidomic markers of sperm cryotolerance in cattle
dc.typeArticle
dspace.entity.typePublication
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Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform–methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography–mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (<jats:italic>P</jats:italic>&lt; 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18 µg) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9 µg;<jats:italic>P</jats:italic>&lt; 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (<jats:italic>P</jats:italic>≤ 0.011). 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