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Apoptotic Effects of Quercitrin on DLD-1 Colon Cancer Cell Line

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dc.contributor.authorÇinçin, Zeynep Birsu
dc.contributor.authorÜnlü, Miray
dc.contributor.authorKıran, Bayram
dc.contributor.authorBireller, Elif Sinem
dc.contributor.authorBaran, Yusuf
dc.contributor.authorÇakmakoğlu, Bedia
dc.date.accessioned2026-01-02T23:12:15Z
dc.date.issued2014-08-06
dc.description.abstractQuercetin, which is the most abundant bioflavonoid compound, is mainly present in the glycoside form of quercitrin. Although different studies indicated that quercitrin is a potent antioxidant, the action of this compound is not well understood. In this study, we investigated whether quercitrin has apoptotic and antiproliferative effects in DLD-1 colon cancer cell lines. Time and dose dependent antiproliferative and apoptotic effects of quercitrin were subsequently determined by WST-1 cell proliferation assay, lactate dehydrogenase (LDH) cytotoxicity assay, detection of nucleosome enrichment factor, changes in caspase-3 activity, loss of mitochondrial membrane potential (MMP) and also the localization of phosphatidylserine (PS) in the plasma membrane. There were significant increases in caspase-3 activity, loss of MMP, and increases in the apoptotic cell population in response to quercitrin in DLD-1 colon cancer cells in a time- and dose-dependent manner. These results revealed that quercitrin has antiproliferative and apoptotic effects on colon cancer cells. Quercitrin activity supported with in vivo analyses could be a biomarker candicate for early colorectal carcinoma.
dc.description.urihttps://doi.org/10.1007/s12253-014-9825-3
dc.description.urihttps://gcris.iyte.edu.tr/bitstream/11147/5417/1/5417.pdf
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/25096395
dc.description.urihttps://dx.doi.org/10.1007/s12253-014-9825-3
dc.description.urihttp://hdl.handle.net/11147/5417
dc.identifier.doi10.1007/s12253-014-9825-3
dc.identifier.eissn1532-2807
dc.identifier.endpage338
dc.identifier.issn1219-4956
dc.identifier.openairedoi_dedup___::5d4fe11332859a5034bd7ffc7df19834
dc.identifier.orcid0000-0001-8165-6164
dc.identifier.orcid0000-0003-3465-1808
dc.identifier.orcid0000-0002-1056-4673
dc.identifier.orcid0000-0001-7960-9131
dc.identifier.pubmed25096395
dc.identifier.scopus2-s2.0-84938214197
dc.identifier.startpage333
dc.identifier.urihttps://hdl.handle.net/20.500.12597/35856
dc.identifier.volume21
dc.identifier.wos000354265700013
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofPathology & Oncology Research
dc.rightsOPEN
dc.subjectMembrane Potential, Mitochondrial
dc.subjectTime Factors
dc.subjectDose-Response Relationship, Drug
dc.subjectL-Lactate Dehydrogenase
dc.subjectCaspase 3
dc.subjectQuercitrin
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectAdenocarcinoma
dc.subjectIn Vitro Techniques
dc.subjectAntioxidants
dc.subjectColon cancer
dc.subjectAntineoplastic agent
dc.subjectCell Line, Tumor
dc.subjectColonic Neoplasms
dc.subjectHumans
dc.subjectQuercetin
dc.subjectAntiproliferative
dc.subjectCell Proliferation
dc.subject.sdg3. Good health
dc.titleApoptotic Effects of Quercitrin on DLD-1 Colon Cancer Cell Line
dc.typeArticle
dspace.entity.typePublication
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