Increasing microplastics pollution: An emerging vector for potentially pathogenic bacteria in the environment

Abstract

Microplastics (MP), plastic particles <5 mm, are of global concern due to their worldwide distribution and potential repercussions on ecosystems and human well-being. In this study, MP were collected from the urbanized Susurluk basin in Türkiye to evaluate their vector function for bacterial biofilms, both in the wet and dry seasons. Bacterial biofilms were predominantly found on polyethylene (PE), polypropylene (PP), and polystyrene (PS), which constitute the most common MP types in the region. Specific potentially pathogenic bacterial genera, including Pseudomonas sp., Comamonas sp., Salmonella spp., and Shigella spp., were prevalent on MP surfaces. Notably, PE and PP harboured numerous genera of potential human and/or animal origin such as Staphylococcus, Proteus, Escherichia, Enterococcus, and Enterobacter. Water quality played a pivotal role in bacterial biofilm formation on MP. Higher salinity in estuarine areas reduced bacterial abundance on MP, while the more polluted freshwater Nilüfer Stream harboured a higher abundance of total bacteria, particularly of potentially pathogenic strains. Seasonal variations, ambient water conditions, and polymer type are all factors that could influence bacterial colonization on MPs. This catchment-wide evaluation, which includes various habitat types (lentic and lotic systems), the enrichment of cultivable viable bacteria on microplastics (MPs) - a key factor in the spread of pathogens - has significant implications for both environmental and public health. Unlike controlled laboratory experiments or in-situ studies with various particles, this study emphasized the dynamic and complex nature of bacterial strains on MPs, which varied depending on seasonal dynamics and antropogenic impacts in open systems. Further research is needed to thoroughly investigate to fully explore the complex interactions among MPs, microbial communities, and their ecological roles, especially in the context of changing environmental factors across entire river catchments.