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The Novel 5-Fluorouracil Loaded Ruthenium-based Nanocarriers Enhanced Anticancer and Apoptotic Efficiency while Reducing Multidrug Resistance in Colorectal Cancer Cells

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dc.contributor.authorDanisman-Kalindemirtas, Ferdane
dc.contributor.authorOzerkan, Dilsad
dc.contributor.authorKariper, Ishak Afsin
dc.contributor.authorBulut, Huri
dc.date.accessioned2026-01-05T23:15:43Z
dc.date.issued2023-02-22
dc.description.abstractRecently, nanocarriers have been made to eliminate the disadvantages of chemotherapeutic agents by nanocarriers. Nanocarriers show their efficacy through their targeted and controlled release. In this study, 5-fluorouracil (5FU) was loaded into ruthenium (Ru)-based nanocarrier (5FU-RuNPs) for the first time to eliminate the disadvantages of 5FU, and its cytotoxic and apoptotic effects on HCT116 colorectal cancer cells were compared with free 5FU. 5FU-RuNPs with a size of approximately 100 nm showed a 2.61-fold higher cytotoxic effect compared to free 5FU. Apoptotic cells were detected by Hoechst/propidium iodide double staining, and the expression levels of BAX/Bcl-2 and p53 proteins, in which apoptosis occurred intrinsically, were revealed. In addition, 5FU-RuNPs was also found to reduce multidrug resistance (MDR) according to BCRP/ABCG2 gene expression levels. When all the results were evaluated, the fact that Ru-based nanocarriers alone did not cause cytotoxicity proved that they were ideal nanocarriers. Moreover, 5FU-RuNPs did not show any significant effect on the cell viability of normal human epithelial cell lines BEAS-2B. Consequently, the 5FU-RuNPs synthesized for the first time may be ideal candidates for cancer treatment because they can minimize the potential drawbacks of free 5FU.
dc.description.urihttps://doi.org/10.1007/s10895-023-03180-9
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/36811696
dc.description.urihttps://avesis.erciyes.edu.tr/publication/details/1bec02e3-529f-40c2-b245-e4ff41e01d89/oai
dc.description.urihttps://hdl.handle.net/20.500.12713/5549
dc.identifier.doi10.1007/s10895-023-03180-9
dc.identifier.eissn1573-4994
dc.identifier.endpage1236
dc.identifier.issn1053-0509
dc.identifier.openairedoi_dedup___::98a513baa16ebde1b719062fd98730e3
dc.identifier.pubmed36811696
dc.identifier.scopus2-s2.0-85148522595
dc.identifier.startpage1227
dc.identifier.urihttps://hdl.handle.net/20.500.12597/43676
dc.identifier.volume33
dc.identifier.wos000936252100002
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofJournal of Fluorescence
dc.rightsCLOSED
dc.subjectColorectal Cancer
dc.subjectRuthenium-Based Nanoparticles
dc.subjectCytotoxicity
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectHCT116 Cells
dc.subjectRuthenium
dc.subjectNeoplasm Proteins
dc.subjectCell Line, Tumor
dc.subjectHumans
dc.subjectATP Binding Cassette Transporter, Subfamily G, Member 2
dc.subjectFluorouracil
dc.subjectColorectal Neoplasms
dc.subjectfu
dc.subject.sdg3. Good health
dc.titleThe Novel 5-Fluorouracil Loaded Ruthenium-based Nanocarriers Enhanced Anticancer and Apoptotic Efficiency while Reducing Multidrug Resistance in Colorectal Cancer Cells
dc.typeArticle
dspace.entity.typePublication
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