Association between MDR 1 (ABCB1) gene C3435T, C1236T, G2677T/A, A2956G polymorphisms and the risk of breast cancer among Turkish Women

Abstract

Objective: The most common malignancy in women is breast cancer worldwide. Previous studies performed in different populations have suggested an association between Multi-Drug Resistance (MDR1) gene polymorphisms and breast cancer risk in women. The purpose of the current study is to examine relationship between MDR1 polymorphisms (C3435T, G2677T/A, C1236T, A2956G) and the risk of breast cancer in Turkish women.Methods: In this study 35 breast cancer cases and 20 healthy controls were enrolled. Identification of MDR1 genotypes was performed with the polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) technique.Results: We observed significant difference in distribution of C3435T genotypes between the cases and the controls (cases, CC 37.1%, CT 28.6%, and TT 34.3%; controls, CC 25%, CT 65%, and TT 10%, p: 0.023). On the other hand, no significant differences in genotype and allele frequencies of C1236T and G2677T/A polymorphisms were observed between groups. We also found that all subjects carry AA genotype for A2956G polymorphism. Conclusion: Although our study group is small, the results suggest that the MDR1 C3435T polymorphism may increase the breast cancer risk in Turkish women.