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Multidirectional insights on Chrysophyllum perpulchrum leaves and stem bark extracts: HPLC-ESI-MSn profiles, antioxidant, enzyme inhibitory, antimicrobial and cytotoxic properties

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dc.contributor.authorBaloglu, Mehmet Cengiz
dc.contributor.authorLlorent-Martínez, Eulogio J.
dc.contributor.authorAumeeruddy, Muhammad Zakariyyah
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.contributor.authorAltunoglu, Yasemin Celik
dc.contributor.authorUstaoglu, Buket
dc.contributor.authorOcal, Mustafa
dc.contributor.authorGürel, Songül
dc.contributor.authorBene, Kouadio
dc.contributor.authorSinan, Kouadio Ibrahime
dc.contributor.authorZengin, Gokhan
dc.date.accessioned2026-01-04T13:21:52Z
dc.date.issued2019-08-01
dc.description.abstractAbstract Chrysophyllum perpulchrum Mildbr. ex Hutch. & Dalziel has been reported to possess several therapeutic properties in African traditional medicine. However, its pharmacological properties have not been fully studied. Herein, we focused on the evaluation of the antioxidant, enzyme inhibitory, antimicrobial and cytotoxic effects of three solvent extracts (ethyl acetate, methanol, and water) of the stem bark and leaves. Polyphenolic components of the extracts were also identified by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). The methanolic stem bark extract possess the highest DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging, ferric and molybdenum (VI) reducing, and the highest inhibitory effect against acetylcholinesterase and butyrylcholinesterase. The aqueous stem bark extract displayed the highest ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) scavenging and cupric reducing power. In contrast, the leaf ethyl acetate extract was the most effective metal chelator and α-amylase inhibitor while the leaf methanolic extract exhibited the highest tyrosinase and α-glucosidase inhibition. The highest total phenolic content (131.58 mg GAE/g extract) was recorded from the methanolic stem bark extract while the leaf methanolic extract was the richest in total flavonoid content (30.43 mg RE/g extract). Flavan-3-ols were main group in the methanol extracts. The minimum inhibitiory concentration values for the methanolic stem bark extract against Gram positive and negative bacteria strains ranged between 62.5–125 μg/ml. Bark extracts of C. perpulchrum were very effective against Salmonella kentucky, Proteus vulgaris and Staphylococcus aureus at 62.5 μg/ml. In addition, cytotoxic activity of stem bark samples was evident at lower concentration than those of leaf extracts. Stem bark extracts displayed the highest prevention against to HeLa cell line (IC50 = 264 μg/mL after 48 h). In light of the above, C. perpulchrum might provide health benefits against oxidative stress and the associated chronic diseases. Nonetheless, the detailed mechanism of action is yet to be further investigated.
dc.description.urihttps://doi.org/10.1016/j.indcrop.2019.03.066
dc.description.urihttps://dx.doi.org/10.1016/j.indcrop.2019.03.066
dc.description.urihttps://hdl.handle.net/20.500.12491/9956
dc.description.urihttps://hdl.handle.net/20.500.12395/38016
dc.identifier.doi10.1016/j.indcrop.2019.03.066
dc.identifier.endpage42
dc.identifier.issn0926-6690
dc.identifier.openairedoi_dedup___::8e12fe081f1a2020779d6a40928514d3
dc.identifier.orcid0000-0003-2976-7224
dc.identifier.orcid0000-0002-0180-5172
dc.identifier.orcid0000-0003-3962-8666
dc.identifier.orcid0000-0003-2379-5363
dc.identifier.orcid0000-0001-8489-116x
dc.identifier.orcid0000-0002-6168-5707
dc.identifier.orcid0000-0001-6548-7823
dc.identifier.scopus2-s2.0-85063567077
dc.identifier.startpage33
dc.identifier.urihttps://hdl.handle.net/20.500.12597/37443
dc.identifier.volume134
dc.identifier.wos000471361200005
dc.language.isoeng
dc.publisherElsevier BV
dc.relation.ispartofIndustrial Crops and Products
dc.rightsOPEN
dc.subjectEnzyme inhibition
dc.subjectAnticancer
dc.subjectEnzyme Inhibition
dc.subjectAfrica
dc.subjectPolyphenolic
dc.subjectAntimicrobial
dc.subjectAntioxidant
dc.subject.sdg6. Clean water
dc.subject.sdg3. Good health
dc.titleMultidirectional insights on Chrysophyllum perpulchrum leaves and stem bark extracts: HPLC-ESI-MSn profiles, antioxidant, enzyme inhibitory, antimicrobial and cytotoxic properties
dc.typeArticle
dspace.entity.typePublication
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