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Investigation of chemical profile, biological properties of Lotus corniculatus L. extracts and their apoptotic-autophagic effects on breast cancer cells

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dc.contributor.authorYerlikaya, Şerife
dc.contributor.authorBaloğlu, Mehmet Cengiz
dc.contributor.authorDiuzheva, Alina
dc.contributor.authorJeko, Jozsef
dc.contributor.authorCziaky, Zoltan
dc.contributor.authorZengin, Gökhan
dc.date.accessioned2026-01-05T23:20:57Z
dc.date.issued2019-09-01
dc.description.abstractThis study aimed to reveal chemical profiles and biological activities of ethyl acetate (EA), methanol (MeOH), and water extracts of Lotus corniculatus. Ethnobotanical reports have indicated the importance of phytochemical properties of the genus Lotus. In this study, the effects of medicinal plant extracts on antioxidant (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelating assays), enzyme inhibitory (on cholinesterase, tyrosinase, a-amylase and a-glucosidase), DNA protection and anticancer properties (including anti-proliferative, cell death and telomerase activity marker gene analysis, apoptotic DNA fragmentation analysis, cell migration test) were evaluated. According to chemical analysis, quercetin derivatives geraldol, isorhamnetin and kaempferol-O-coumaroylhexoside-O-deoxyhexoside isomers were dominant in the extracts. MeOH extracts showed the highest total flavonoids capacity with 21.13 mg RE/g. EA extract showed the strongest anti-amylase activity among the tested extracts. Water extract had the most protective activity against plasmid DNA. To indicate cell survival, MTT test was performed against human MCF-7 and MDA-MB-231 breast cancer cells. Half-maximal inhibitory concentration for cells were calculated and used for detection of mechanisms behind the cancer cell death. EA extract showed up-regulation of Bax proapoptotic gene and apoptotic DNA fragmentation activity on highly invasive MDA-MB-231 cells. Beclin-1 and LC3-II autophagy genes were higly expressed after treatment of MCF-7 cells with EA extracts. EA and MeOH extracts inhibited cell migration ability of both cancer cells. Linoleamide, was dominant component in EA extract and caused apoptosis on MDA-MB-231 breast cancer cells via increasing intranuclear Ca²+. The detailed mechanism behind the anticancer properties should be further investigated.
dc.description.urihttps://doi.org/10.1016/j.jpba.2019.05.068
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/31185340
dc.description.urihttps://dx.doi.org/10.1016/j.jpba.2019.05.068
dc.description.urihttps://hdl.handle.net/20.500.12395/37942
dc.identifier.doi10.1016/j.jpba.2019.05.068
dc.identifier.endpage299
dc.identifier.issn0731-7085
dc.identifier.openairedoi_dedup___::b0b69521fa87363df8c00d514f49f755
dc.identifier.orcid0000-0002-5349-7298
dc.identifier.orcid0000-0001-6548-7823
dc.identifier.pubmed31185340
dc.identifier.scopus2-s2.0-85066857240
dc.identifier.startpage286
dc.identifier.urihttps://hdl.handle.net/20.500.12597/43734
dc.identifier.volume174
dc.identifier.wos000479328000035
dc.language.isoeng
dc.publisherElsevier BV
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis
dc.rightsOPEN
dc.subjectChemical ingredients
dc.subjectMelanoma, Experimental
dc.subjectApoptosis
dc.subjectBreast Neoplasms
dc.subjectDNA Fragmentation
dc.subjectAcetates
dc.subjectAntioxidants
dc.subjectExtracts
dc.subjectMice
dc.subjectCell Line, Tumor
dc.subjectAutophagy
dc.subjectAnimals
dc.subjectHumans
dc.subjectFlavonoids
dc.subjectMethanol
dc.subjectAntineoplastic Agents, Phytogenic
dc.subjectOxidative Stress
dc.subjectAmylases
dc.subjectLotus
dc.subjectMCF-7 Cells
dc.subjectFemale
dc.subjectDrug Screening Assays, Antitumor
dc.subject.sdg3. Good health
dc.titleInvestigation of chemical profile, biological properties of Lotus corniculatus L. extracts and their apoptotic-autophagic effects on breast cancer cells
dc.typeArticle
dspace.entity.typePublication
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