Browsing by Author "Yetgin A."

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Antimicrobial activity and chemical composition screening of Anacyclus pyrethrum root
(2017-07-01) Canli K.; Yetgin A.; Akata I.; Altuner E.
Medical herbs have many bioactive component and they are used in microbial treatment since ancient times. The resistance of pathogens to antibiotics became a critical problem, so researches for novel antimicrobial agents are required. Anacyclus pyrethrum (pellitory, Spanish chamomile or Mount Atlas daisy) is commonly used as a traditional medicine, therefore the antimicrobial activity of the root of this medicinal plant was investigated against 17 bacteria and 1 fungi by using disk diffusion method. The test strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy analysis. The results were presented that A. pyrethrum has antimicrobial activity against all tested microbial species except E. faecalis and S. typhimurium. Eighteen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed NMR spectra for 3D structure determination and identification.
Antimicrobial activity and chemical composition screening of Epilobium montanum root
(2017-07-01) Canli K.; Yetgin A.; Akata I.; Altuner E.
Medical herbs have many bioactive component and they are used in microbial treatment since ancient time. The resistance of pathogen to antibiotic is became a critical problem, so novel antimicrobial agent related research is required. Epilobium montanum related antimicrobial research doesn’t exist, therefore root of this medicinal plant investigation was applied against 17 bacteria and 1 fungi by using disk diffusion method. These microbial species include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy. The results were presented that E. montanum has antimicrobial activity against all tested microbial species except E. coli. Seventeen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed by NMR spectra for 3D structure determination and identification.
Biochemical composition and in vitro antimicrobial activity of endemic helichrysum arenarium ssp. aucheriethanol extract
(2021-02-01) Bozyel M.E.; Canli K.; Benek A.; Yetgin A.; Altuner E.M.
The phenomenon of using plants for therapeutic purposes dates back to the earlier times in the history of humanity. Even though there have been remarkable developments in the modern medicine, pharmaceutical, and chemical industries, the therapeutic use of medicinal plants is still up to date. Due to the emergence of antibiotic-resistant strains and the proliferation of side effects in synthetic drugs, the importance of research on the antimicrobial potential and biochemical composition of plant extracts has increased. The main purpose of this present study is to screen both the antibacterial and antifungal activity of endemic Helichrysum arenarium ssp. aucheri ethanol extract by disk diffusion on some Gram-negative and Gram-positive bacteria and Candida albicans, and to identify the major compounds found in the ethanol extract by GC/MS. According to the results obtained from the study, it can be proposed that the extract showed antibacterial and anti-fungal activity for all microorganisms excluding Salmonella typhimurium SL1344, Salmonella infantis, E. coli (food isolate), Escherichia coli ATCC 25922 and Enterobacter aerogenes ATCC 13048, and some chemical compounds were detected, which may be responsible for the antimicrobial activity.
Chemical composition and in vitro antimicrobial activity of matthiola tricuspidata ethanol extract
(2021-10-01) Canli K.; Bozvel M.E.; Benek A.; Yetgin A.; Senturan M.; Altuner E.M.
It is widely acccptcd by scientists that innovation of new antimicrobial agents has extreme im-portance for the sake of the tiiturc of human beings; therefore the researches about the antimicrobial potentials of plant extracts and their biochemical composition have great importance. This present study aims to investigate the antimicrobial activity of Mat-thiola tricuspidata ethanol extract against several Gram-positive and Gram-negative microorganisms and Candida albicans by disk diffusion method, and to identity its chemical composition by GasChroma-tography-Mass Spectrometry. As a result, it was observed that the cxtract showed activity for all microorganisms excluding Enterobacter aerogenes ATCC 13048, Escherichia coli ATCC 25922, E. coli, Klebsiella pneumoniae, Salmonella infantis, and Salmonella typhimurium SL1344 and some chcmical compounds were dctcctcd, which may be responsible for the antimicrobial activity.
Comparison of Antimicrobial Activity of Allium sativum Cloves from China and Taşköprü, Turkey
(2018-01-01) Yetgin A.; Canli K.; Altuner E.
In this study, antimicrobial activities of two different samples of Allium sativum L. from Turkey (TR) (Taşköprü, Kastamonu, Turkey) and China (CN) were determined. A broad spectrum of Gram-negative and Gram-positive bacteria (17 bacteria) including species of Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, and Staphylococcus were used for testing antibacterial activity. In addition, antifungal activity against Candida albicans was also investigated. Antimicrobial activity was tested by using 3 different processes (chopping, freezing, and slicing by the disk diffusion method). The results showed that TR garlic presented more antimicrobial activity than CN garlic. Mechanism of activity of CN garlic could be proposed to be different from that of TR garlic.
Determination of the chemical composition and antimicrobial activity of Frankenia hirsuta
(2017-12-24) Canli K.; Şimşek Ö.; Yetgin A.; Altuner E.
Frankenia hirsuta is widely located in Turkey, but the antimicrobial potential and biochemical composition analysis of it weren’t determined yet. By using the disk diffusion method, the susceptibility of 17 bacteria and 1 fungi were analyzed, which included Bacillus, Candida, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella and Staphylococcus genera. 0.8, 1.5 and 3.1 mg of samples were prepared by using absolute ethanol. The bioactive composition of the plant extract was determined by gas chromatography- mass spectroscopy, and National Institute of Standards and Technology library was used for the mass spectra analysis. The results showed that F. hirsuta had antimicrobial activity against all of the studied micro-organisms except E. aerogenes and E. coli. Several active metabolites were identified, but some composition of this sample didn’t match with the library. These results are the first report for the antimicrobial potential and biochemical composition of F. hirsuta.
In Vitro Antimicrobial Activity Screening of Ethanol Extract of Lavandula stoechas and Investigation of Its Biochemical Composition
(2019-01-01) Canli K.; Yetgin A.; Benek A.; Bozyel M.; Altuner E.
The aim of this study was to test antimicrobial activity of ethanol extract of Lavandula stoechas against 22 bacteria and 1 yeast. Also, biochemical composition of the extract was investigated. A wide range of Gram-positive, Gram-negative microorganisms, and multidrug resistant bacteria were selected to test the antimicrobial activity. As a result, the extract is observed to contain fenchone (bicyclo[2.2.1]heptan-2-one, 1,3,3-trimethyl-, (1R)-) and camphor (+)-2-bornanone) as major components and showed antimicrobial activity against all studied microorganisms except Escherichia coli ATCC 25922 and Klebsiella pneumoniae. The results of the study present that L. stoechas is active against MDR strains too.
In vitro antimicrobial screening of Aquilaria agallocha roots
(2016-01-01) Canlı K.; Yetgin A.; Akata I.; Altuner E.M.
Background: It was previously shown that some parts of Aquilaria agallocha, which is commonly known as oud or oodh, such as roots have been used as a traditional medical herbal in different countries. In Turkey A. agallocha is one of the ingredients while preparing famous Mesir paste, which was invented as a medicinal paste and used from the Ottoman period to now at least for 500 years. The identification the in vitro antimicrobial activity of ethanol extract of A. agallocha roots is main purpose of this analysis. Materials and Methods: By using 17 bacteria and 1 fungi, which include Bacillus, Candida, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella and Staphylococcus genera, the activity of A. agallocha root extracts were analysed by the help of the disk diffusion method, that is one of the methods commonly used to determine antimicrobial activities. Results: As a result of the study it was observed that ethanol extracts of A. agallocha roots have a clear antimicrobial activity against nearly all microorganism used in the study, but only two bacteria namely E. coli ATCC 25922 and S. typhimurium SL 1344. Conclusion: According to the disk diffusion test results it may be possible to propose that A. agallocha roots should have a medicinal uses especially against E. faecium, L. monocytogenes ATCC 7644, B. subtilis DSMZ 1971, C. albicans DSMZ 1386, S. epidermidis DSMZ 20044 and S. aureus ATCC 25923.