Browsing by Author "Verep D."

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Antimicrobial activities and some flavonoids in extracts of some medicinal plants
(2017-07-01) Şener I.; Gür M.; Verep D.; Güney K.; Altuner E.
Origanum majorana, Melissa officinalis, Anthemis cotula and Avena sativa were extracted by using 65% ethanol to isolate their active constituents. The antimicrobial activities of extracts were investigated against 15 microorganisms by using the disk diffusion method, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) and MFC (Minimal Fungicidal Concentration) tests. Furthermore, the presence of some flavonoids were analyzed by using HPLC. It was determined flavonoids in the extracts of O. majorana, M. officinalis, A. cotula and A. sativa. As a results it was observed that O. majorana was active against Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis, Salmonella typhimurium and Pseudomonas aeruginosa, where M. officinalis was active against Enterococcus faecium and Enterococcus faecalis. On the other hand, both A. cotula and A. sativa were observed to be active against Enterococcus faecium. The extracts of plant samples showed antibacterial activity against tested microorganisms at different levels.
Determination of some flavonoids and antimicrobial behaviour of some plants’ extracts
(2017-07-01) Gür M.; Verep D.; Güney K.; Güder A.; Altuner E.
C. sativa, C. intybus, L. stoechas, V. officinalis and G. glabra plants were extracted by using 65% ethanol to isolate their active constituents. The antimicrobial activities of extracts were investigated against 15 microorganisms by using the disk diffusion method, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) and MFC (Minimal Fungicidal Concentration) tests. Furthermore, the presence of eight flavonoids were analysed by using HPLC. It was found that C. sativa is active against C. albicans, E. faecalis, S. enteritidis and S. typhimurium with MIC values of 26.02 µg/mL, 13.01 µg/mL, 416.25 µg/mL and 832.50 µg/mL respectively, where C. intybus is active against C. albicans and E. faecalis, with MIC values of 13.01 µg/mL and 6.50 µg/mL, respectively. On the other hand, L. stoechas and V. officinalis were observed to be active against only S. enteritidis with MIC values of 52.03 µg/mL and 26.02 µg/L respectively, where G. glabra was active against only E. faecalis, with a MIC value of 52.03 µg/mL. The extracts of plant samples showed antibacterial activity against tested microorganisms at different levels. But the activities against C. albicans and E. faecalis is noteworthy. The flavonoids were determined at different amounts in extracts.
Some Important Plants for Epilepsy Treatment: Antioxidant Activity and Flavonoid Compositions
(2018-12-01) Gür M.; Güder A.; Verep D.; Güney K.; Özkan O.E.; Seki N.; Kandemirli F.
Epilepsy, a disorder of the brain, is a major health problem that the widespread availability and use of herbal medicines raise the potential for adverse effects in the epilepsy population. Although many synthetic antiepileptic drugs are available in operation, their efficiency does not verify with the all population suffering from this disorder because of the major restrictions such as side effects and the drug interactions in its clinical utility. This paper is concerned with antioxidant activity, total phenolic and flavonoid contents and flavonoid composition of the 14 plants for three different solvents (water, ethanol and hexane). For this reason, FRAP, DPPH free radical scavenging, metal-chelate and H2O2 scavenging activities were investigated. In addition, total phenolic and flavonoid contents of extracts were determined via spectroscopic techniques. Lastly, composition of catechin, epicatechin, rutin, naringin, myricetin, luteolin, naringenin and apigenin was found with normal-phase HPLC in the obtained extracts. Extracts of fourteen plants taxa foreseen in having antioxidant and antiepileptic features were obtained with soxhlet extraction using different solvents, i.e., water, ethanol and hexane in this study. The extracts of 14 plant taxa were acquired: French lavender (Lavandula stoechas L.—inflorescence), Valerian (Valeriana officinalis L.—herb), Syrian rue (Peganum harmala L.—seeds), Liquorice (Glycyrrhiza glabra L.—roots), Boxwood (Buxus sempervirens L.—leaves), Chicory (Cichorium intybus L.—herb), Marjoram (Origanum majorana L.—leaves), Lemon balm (Melissa officinalis L.—leaves), Prickly juniper (Juniperus oxycedrus L.—fruits), Wild camomile (Anthemis cotula L.—flowers), Oats (Avena sativa L.—herb), Coriander (Coriandrum sativum L.—seeds), Flax (Linum usitatissimum L.—seeds) and Marijuana (Cannabis sativa L.—seeds)”. The antioxidant capacities of plant extracts were assayed with four different assays including FRAP, metal chelating, H2O2, DPPH scavenging because the antioxidant capacity cannot be fully described by a single method. The determined flavonoid amounts are between 3.85 and 694-47 (catechin), 2.70 and 2045.49 (epicatechin), 0.12 and 47.11 (rutin), 0.02 and 24.99 (naringin), 0.80 and 153.19 (myricetin), 0.03 and 7.80 (luteolin), 0.02 and 4.75 (naringenin) and 0.38 and 2.73 (apigenin) as µg g−1 plant. These plant materials and their flavonoid components via gamma-aminobutyric acid (GABA) and GABAA receptors can be therapeutical due to their different mechanism on treatment of epilepsy.