Browsing by Author "Ugurlu A."

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Bioinformatics and its utilization in green biotechnology
(2020-01-01) Altunoglu Y.C.; Ugurlu A.; Baloglu P.; Baloğlu M.C.
Bioinformatics use computer technology to manage and process those data from biological experiments. Many bioinformatics methods have been developed to compute those huge data by genomics. Collection, storage and application of bioinformatics methods of plant genetic resources can contribute to the production of plants with higher crop quality and resistant to abiotic stresses, diseases and insects. These approaches can lead to understanding of biological systems.
Multi-targeted potential of Pittosporum senacia Putt.: HPLC-ESI-MSⁿ analysis, in silico docking, DNA protection, antimicrobial, enzyme inhibition, anti-cancer and apoptotic activity
(2019-12-01) Mahomoodally M.; Picot-Allain C.; Hosenally M.; Ugurlu A.; Mollica A.; Stefanucci A.; Llorent-Martínez E.; Baloglu M.; Zengin G.
Pittosporum senacia (PS) Putt. (Pittosporaceae), indigenous to the Mascarene Islands, is a common ingredient in traditional medicines. However, there is currently a dearth of studies to validate some of these traditional claims. Given the broad traditional uses of PS against several diseases, we aimed to provide a comprehensive insight into the biological and chemical profile of P. senacia. The antioxidant, enzyme inhibitory activity, anticancer, and phytochemical composition of the methanolic extract of P. senacia leaf extracts were studied. The possible interaction and binding mode of the most abundant phytochemicals were studied via in silico docking experiments on tyrosinase and α-glucosidase. The mechanism behind the cytotoxic property of P. senacia extract for MDA-MB-231 was also examined using different methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability test checking apoptosis-associated genes, and wound healing assays. Twenty-six compounds were identified, of which caffeoylquinic acid derivatives, ferulic acid derivative, cinnamoylquinic acid derivative and two other polyphenols (oleuropeine and isoramnetin glucoside) being abundant, have been tested using in silico studies, against α-glucosidase and tyrosinase. The extract (IC50 = 118.8 μg/ml) exhibited time and dose dependent anti-proliferative effect on human breast cancer cell line, MDA-MB-231. According to the expression profile of apoptosis inhibitors and apoptosis promoters genes, expression of Bax and Bak genes were significantly increased compared to Bcl-2 and Birc5 genes. Based on wound healing analysis, cell migration was inhibited after the application of the plant extract. The present findings suggested that PS might be a good candidate as sources of bioactive compounds for designing functional applications.
Novel in vitro and in silico insights of the multi-biological activities and chemical composition of Bidens tripartita L.
(2018-01-01) Uysal S.; Ugurlu A.; Zengin G.; Baloglu M.C.; Altunoglu Y.C.; Mollica A.; Custodio L.; Neng N.R.; Nogueira J.M.F.; Mahomoodally M.F.
Bidens tripartita L. is a traditional phyto-remedy used in several countries, yet there is still a paucity of data on its biological potential. We aimed to provide new insights on the pharmacological potential of extracts prepared from B. tripartita via highlighting its antioxidant, key enzymes inhibitory potency, and DNA protecting effects. Phytochemical profile was established using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) and bioactive compound(s) docked against target enzymes using in silico methods. Cytotoxicity against three cancer cell lines was assessed using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) cell viability test. The main compounds were luteolin-7-glucoside (cynaroside), chlorogenic acid, and epicatechin in the extracts. The methanol extract exhibited the highest radical scavenging activity. Ethyl acetate extract showed strongest α-amylase inhibitory activity, while the best α-glucosidase inhibitory effect recorded for the methanol extract. Molecular docking showed that cynaroside strongly interact to α-glucosidase cavity by establishing six hydrogen bonds. B. tripartita extracts were found to protect supercoiled form of pUC19 plasmid (>70%) and also showed anti-proliferative properties. Results amassed in the present study add on to a growing body of literature on the multi-pharmacological potency of B. tripartita which can be applied to bio-products development geared towards management of common diseases.
Syzgium coriaceum Bosser & J. Guého—An endemic plant potentiates conventional antibiotics, inhibits clinical enzymes and induces apoptosis in breast cancer cells
(2020-01-01) Mahomoodally M.; Ugurlu A.; Llorent-Martínez E.; Nagamootoo M.; Picot-Allain M.; Baloglu M.; Altunoglu Y.; Hosenally M.; Zengin G.
Syzygium species are renowned for being important reservoirs of phytochemicals with pharmaceutical and biomedical potential. However, no attempt has been made to delineate the pharmacological potential and phytochemical profile of Syzgium coriaceum Bosser & J. Guého, an endemic plant to Mauritius. The present study aimed to determine the antibacterial, antioxidant, cytotoxicity, enzyme inhibitory and phytochemical profile of the ethyl acetate and methanol extracts of S. coriaceum. Preliminary qualitative phytochemical study of the extracts showed the presence of phenol, tannins, and alkaloids. Chemical characterisation showed the presence of derivatives of tannins, gallic acids, quercetin, and kaempferol. Potentiating activity between S. coriaceum extracts and antibiotics (ampicillin and streptomycin) using the checkerboard method showed additive interaction. The extracts showed potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) (2.95 and 2.93 mmol trolox equivalent (TE)/g sample for ethyl acetate and methanol extracts, respectively) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) (4.09 and 3.83 mmol TE/g sample for ethyl acetate and methanol extracts, respectively) scavenging abilities. Syzygium coriaceum extracts were active inhibitors of α-glucosidase (about 47 mmol acarbose equivalent/g sample for ethyl acetate and methanol extract). S. coriaceum methanol extract caused maximum inhibition against human breast adenocarcinoma (MDA-MB-231) cancer cells after 48 h treatment with the IC50 value of 53.41 μg/mL. Expression of anti-apoptotic Bcl2 and BIRC5 genes were down-regulated. It can be concluded that S. coriaceum extracts lead to MDA-MB-231 cells apoptosis. This investigation has provided a comprehensive report of the biological and chemical profile of S. coriaceum. Collected scientific evidences can open new avenues for research and contributes towards establishing primary data on Syzygium species endemic to Mauritius for bioprospection of novel phytopharmaceuticals.