Browsing by Author "Tumer K.C."

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Investigation of canine chaphamaparvovirus, canine bufavirus, and canine adenovirus in dogs with diarrhea: First report of novel canine bufavirus in Turkey
(2023-01-01) Abayli H.; Aslan O.; Tumer K.C.; Can-Sahna K.; Tonbak S.
Viral enteritis is a significant cause of death among dogs younger than 6 months. In this study, the presence of canine chaphamaparvovirus (CaChPV), canine bufavirus (CBuV), and canine adenovirus (CAdV) was investigated in 62 diarrheal dogs previously tested for other viral pathogens (canine parvovirus type 2, canine coronavirus, and canine circovirus). CBuV was detected in two dogs (3.22%) and CaChPV in one dog (1.61%). One dog tested positive for three parvoviruses (CPV-2b, CBuV, and CaChPV). All dogs tested negative to CAdV-1/CAdV-2. A long genome fragment of one of the two identified CBuVs and of the CaChPV was obtained and analyzed. New Turkish CBuVs had high identity rates (96%–98% nt; 97%–98% aa) with some Italian CBuV strains (CaBuV/9AS/2005/ITA and CaBuV/35/2016/ITA). The phylogenetic analysis powerfully demonstrated that these viruses belonged to a novel genotype (genotype 2). A part of the genome ChPV-TR-2021–19 revealed high identity rates (> 98% nt and > 99% aa) with some Canadian CaChPV strains (NWT-W88 and NWT-W171) and the Italian CaChPV strain Te/37OVUD/2019/IT. This study is the first report on the detection of CBuV-2 and the concomitant presence of three canine parvoviruses in Turkey. The obtained data will contribute to the molecular epidemiology and the role in the etiology of enteric disease of new parvoviruses.
Serum cardiac troponin I concentration increases in sheep with uterine torsion
(2022-11-01) Tumer K.C.; Safak T.
It is currently unclear whether myocardial damage occurs in sheep suffering from uterine torsion. Increased serum cardiac troponin I (cTnI) concentration is considered as the gold standard indicator of myocardial damage. The purpose of this study was to investigate the serum cTnI concentrations in sheep with uterine torsion and the relationship of serum cTnI concentrations in cases of uterine torsion to hematological and biochemical variables, the degree of uterine torsion and the estimated duration of labor. A total of 32 sheep were included in the study and were assigned to uterine torsion (UT, n = 14), other types of dystocia requiring cesarean section or manual assistance (OTD, n = 11), and unassisted vaginal delivery (UVD, n = 7) groups based on inclusion criteria. Advia Centaur TnI-Ultra, a human-specific immunoassay, was used to measure serum cTnI concentrations. The median serum cTnI concentration was significantly higher in the UT group than in the OTD and UVD groups (UT: 0.507 ng/mL (0.040–18.521); OTD: 0.044 ng/mL (0.016–0.135); UVD: 0.036 ng/mL (0.023–0.059); p < 0.001). In uterine torsion cases, serum cTnI concentrations correlated positively with serum CK (r = 0.556, p = 0.042), AST (r = 0.613, p = 0.022) and LDH activities (r = 0.609, p = 0.024), and degree of uterine torsion (r = 0.563, p = 0.036). The results indicated that uterine torsion leads to an increase in serum cTnI concentration. Further investigations can be undertaken to explore possible factors contributing to the release of cTnI from the myocardium into the circulation and whether the myocardial damage in uterine torsion is reversible or irreversible.
The relationship between serum cytokine profile and vitamin D in calves with neonatal diarrhea
(2023-05-01) Caliskan M.; Dabak M.; Tumer K.C.
It is important to know the characteristics of the immunological response in newborn calf diarrhea, which is often caused by bacterial, viral and protozoal pathogens. Cytokines are proteins that serve as chemical messengers to regulate the innate and adaptive arms of the immune response. Changes in circulatory cytokine levels provide valuable information for understanding the pathophysiological process and monitoring disease progression and inflammation. Vitamin D has important immunomodulatory effects, which include enhancing the innate immune system and inhibiting adaptative immune responses. The objective of this study was to evaluate the relationship between serum cytokine profile and vitamin D level in neonatal calves with diarrhea. The study population was comprised of 40 neonatal calves, 32 of which had diarrhea and 8 of which were healthy calves. The calves with diarrhea were allocated to four groups according to bacterial (Escherichia coli), viral (Rotavirus, Coronavirus) and protozoal (Cryptosporidium parvum) etiologies. Circulatory vitamin D metabolites (25-hydroxyvitamin D, 1,25-dihydroxyvitamin D) and cytokines (TNF-α, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13 and IL-17) in the calves were determined. There was no statistically significant difference among the groups in 25-hydroxyvitamin D levels. 1,25-dihydroxyvitamin D levels were higher in Coronavirus and E. coli groups compared to the controls. Serum levels of all cytokines except for IL-13, were higher in E. coli group than those of the control group. As a result, differences in serum cytokines and vitamin D levels according to etiological factors in calf diarrhea indicate that vitamin D may play a role in the immune response in the disease.
Urinary Neutrophil Gelatinase-Associated Lipocalin, Cystatin C and Clusterin as Biomarkers for Acute Kidney Injury in Cattle with Tropical Theileriosis
(2023-01-01) Tumer K.C.; Dincer P.F.P.; Babacan S.; Yerlikaya Z.