Browsing by Author "Tatar M."

Now showing 1 - 6 of 6

An investigation of the endoplasmic reticulum stress in obesity exposure in the prenatal period
(2023-12-01) Tüfekci̇ K.K.; Tatar M.; Terzi̇ F.; Bakirhan E.G.
Expression of ADAMTS 1-4-8 and placental growth factor in ovary and oviduct during pregnancy in the first trimester
(2023-01-01) Tatar M.; Tufekci K.K.; Uslu S.; Öner J.
A Disintegrin and Metalloprotease Domains with Thrombospondins Motifs (ADAMTS), proteinases responsible for the destruction of extracellular matrix structures, have essential roles in the physiological and pathological processes of the female reproductive system, which is a dynamic structure. This study aimed to evaluate the immunoreactivity of placental growth factor (PLGF) and ADAMTS' (1, -4, and -8) in the ovary and oviduct during pregnancy in the first trimester. Our findings suggest a predominant role of ADAMTS-4 and ADAMTS-8 as proteoglycan-degrading enzymes from the ADAMTS-1 in the first trimester. As an angiogenic factor, PLGF showed more immunoreactivity than ADAMTS-1 in the ovary. This study provides the first evidence that ADAMTS-4 and ADAMTS-8 are more expressed in ovarian cells and follicles at different developmental stages during the first trimester of pregnancy than ADAMTS-1. Consequently, we suggest that ADAMTSs and PLGF act together and may exert specific effects on the formation, stabilisation, and function (or a combination thereof) of the matrix surrounding and protecting the follicles.
Expression of placental growth factor and a disintegrin and metalloprotease with a thrombospondin type motifs 1-4-8 during the three trimesters of rat pregnancy at the maternal-fetal interface
(2023-01-01) Tatar M.; Uslu S.; Öner J.
Oleuropein Mitigates Acrylamide-Induced Nephrotoxicity by Affecting Placental Growth Factor Immunoactivity in the Rat Kidney
(AVES, 2023) Tüfekci K.K.; Tatar M.
objective: Oleuropein is one of the main components of the antioxidant properties of olive leaves. Placental growth factor is an important regulator in angiogenesis and inflammation, its levels being variable in pathological conditions. In this study, we aimed to examine changes in placental growth factor expression and the effect of oleuropein, found in olive leaves, in rats exposed to acrylamide nephrotoxicity. Material and Methods: Twenty-four male Wistar albino rats were allocated into 4 groups. The control group received saline solution only. The oleuropein group received oleuropein (4.2 mg/kg), the acrylamide group received acrylamide (5 mg/kg), and the acrylamide and oleuropein group received acrylamide (5 mg/kg) and oleuropein (4.2 mg/kg). All substances were administered via gastric gavage for 21 days. Kidney tissues were removed at the end of the study and subjected to histopathological, stereological, and immunohistochemical procedures. Results: Histopathological examination revealed dilatation, vacuolization, and degeneration in the proximal and distal tubules and increased placental growth factor immunoreactivity in the acrylamide group. Cavalieri volume analysis revealed increased cortex, distal, and proximal tubule volumes (P [removed]
Protective and therapeutic role of melatonin against tunicamycininduced ER stress in testicular tissue of rats
(2022-02-01) Tatar M.; Eren Ü.
Objective(s): This study aimed to investigate the possible consequences of administering exogenous melatonin as prevention or treatment against tunicamycin-induced endoplasmic reticulum (ER) stress in the testicular tissue of rats. Materials and Methods: In this study, 42 adult Sprague Dawley rats, randomly divided into seven equal groups, were administered intraperitoneal tunicamycin to induce ER stress. Both prophylactic (PMel) and therapeutic melatonin (TMel) groups were administered melatonin for seven days. ER stress in the cell was detected through immunohistochemical and molecular analyses using GPR78 expression. Results: Increased oxidant levels and apoptosis rates were shown in testicular tissue because of ER stress. The sections in the melatonin-administered and control groups were similar, with melatonin-administered groups showing an increase in the antioxidant ratio. Histometric examinations revealed both TMel and melatonin applications reduced the diameter of the tubules. However, immunohistochemical and molecular analyses showed that PMel administration decreased the concentration of GRP78 more effectively than TMel. Conclusion: Applying melatonin prior to cell damage occurrence can be recommended for its effectiveness in protecting from tunicamycin-induced ER stress.
Protective Effect of Myricetin Against Experimentally Induced Torsion in Rats
(Pleiades Publishing, 2023) Tatar M.; Polat Z.; Öner J.; Öner H.
esticular torsion causes ischemia and free radical formation by obstructing vascular flow caused by complete or partial rotation of the testis. Myricetin is a plant-derived flavonoid with various biological effects, such as potent anti-oxidation and anti-inflammatory properties. The study aims to evaluate the protective effects of myricetin on the apoptosis of testicular germ cells after experimental testicular torsion. Twenty-four Wistar albino rats were divided into four groups; Control, Sham, Torsion group (720 deg torsion for 2 h), and Myricetin group (torsion model was created, and myricetin injection (1.5 mg/kg) was done during seven days). End of the experiment, testis tissues were removed and fixed in Bouin’s solution. Crossman’s triple stain was done to determine the histological changes. Also, 8-OHdG and Caspase-3 immunoreactivities were assessed, and TUNEL staining was done. According to the results, we found that 8-OHdG, Caspase-3 immunoreactivity, and TUNEL-positive cells increased, accompanied by several histopathological changes in the torsion group. In contrast, myricetin significantly attenuated testicular injury by reducing apoptosis and DNA damage. These findings suggest that myricetin protects testes against torsion-induced damage in rats, possibly by reducing oxidative stress and inhibiting apoptosis. Our study provides a theoretical basis for further research into maintaining male reproductive health.