Browsing by Author "Tastan, Y."

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    Web of Science
    Medicine designed to combat diseases of affluence affects the early development of fish. How do plastic microparticles contribute?
    (2023.01.01) Medkova, D.; Hollerova, A.; Blahova, J.; Marsalek, P.; Mares, J.; Hodkovicova, N.; Doubkova, V.; Hesova, R.; Tichy, F.; Faldyna, M.; Tastan, Y.; Kotoucek, J.; Svobodova, Z.; Lakdawala, P.
    The incidence of diseases of affluence, such as diabetes mellitus, cardiovascular diseases, high blood pressure, and high cholesterol has been reported to rise. Consequently, the concentrations of residues of drugs designed to treat these diseases have been rising in water bodies. Moreover, the toxicity of these pharmaceuticals towards fish and other non-target organisms can be even enhanced by microplastic particles that are reportedly present in surface water. Therefore, the aim of this study was to describe the effects of three highly prescribed drugs, in particular metoprolol, enalapril, and metformin on fish early-life stages. Also, it was hypothesized that polystyrene microparticles will increase the toxicity of metoprolol to fish early-life stages. Embryonal acute toxicity tests on Danio rerio and Cyprinus carpio were carried out in order to describe the possible toxic effects of metoprolol, enalapril, and metformin. Also, the acute toxicity of polystyrene microparticles and the combination of metoprolol with polystyrene microparticles were tested on D. rerio embryos. Additionally, a 31-day long embryo-larval subchronic toxicity test was carried out with C. carpio in order to describe the long-term effects of low concentrations of metoprolol. The results of the study show that both metoprolol and enalapril have the potential to disrupt the early development of the heart in the embryonal stages of fish. Also, enalapril and metformin together with polystyrene microparticles seem to possibly disrupt the reproduction cycle and act as endocrine disruptors. Both pure polystyrene microparticles and the combination of them with metoprolol affect inflammatory processes in organisms. Additionally, metformin alters several metabolism pathways in fish early life stages. The results of the study bring new evidence that even low, environmentally-relevant concentrations of pharmaceuticals have the potential to disrupt the early development of fish, particularly on a molecular level.
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    Web of Science
    Prevalence, molecular identification and genotyping of the crayfish plague pathogen, Aphanomyces astaci in major narrow-clawed crayfish (Pontastacus leptodactylus Eschscholtz, 1823) populations from Türkiye
    (2024.01.01) Akhan, S.; Çagatay, I.T.; Berber, S.; Tastan, B.; Tastan, Y.; Dalar, T.
    Introduction Crayfish plague is considered the most important crayfish disease globally. It is caused by the fungus-like agent, Aphanomyces astaci. This study aimed to identify and determine the prevalence of A. astaci using PCR in narrow-clawed crayfish (Pontastacus leptodactylus) populations from across T & uuml;rkiye.Material and Methods A PCR was carried out with primers specific to the internal transcribed spacer region of the A. astaci pathogen on both telson and abdominal cuticle tissues from crayfish individuals from 41 different locations.Results Aphanomyces astaci was detected in the crayfish from 34 of the locations. Molecular diagnosis showed the prevalence rates of A. astaci to be between 0% and 68.2%. For 7 of the 34 locations, the strain of A. astaci was determined. Microsatellite analysis of tissue from individuals with positive PCR results revealed the A. astaci genotypes in seven populations. Genotype B was found to be the predominant genotype responsible for crayfish plague in Turkish crayfish populations. The Psl genotype (genotype B) was determined in six of the populations, and the As genotype (genotype A) was detected in only one.Conclusion Crayfish plague poses a significant threat to crayfish populations, necessitating the development of rapid, highly sensitive diagnostic methods. An understanding of the sensitivity of the PCR detection method and of the prevalence and genotyping of A. astaci in Turkish crayfish populations has been gained from this study.