Browsing by Author "TATAR, M."

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Oleuropein attenuates placental growth factor expression by regulating oxidative stress and apoptosis in acrylamide hepatotoxicity
(Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Dergisi, 2023-08-31) TATAR, M.; TÜFEKCİ, K. K.
The liver is susceptible to toxic effects, as it is the main site of acrylamide biotransformation and detoxification. Researchers have claimed that placental growth factor (PlGF) and its pathway are potentially involved in numerous diseases, including liver fibrosis and angiogenesis. Oleuropein is a natural phenolic compound with potent antioxidant effects. The purpose of this study was to examine the role of PlGF and the potential protection provided by oleuropein in acrylamide hepatotoxicity. Wistar albino rats were assigned into control, acrylamide (ACR) (5 mg/kg), oleuropein (OLE) (4.2 mg/kg), and ACR+OLE groups. Acrylamide and oleuropein were administered for 21 days. The control group received only physiological saline. Liver tissues were evaluated histologically and immunohistochemically. Histological examinations revealed significant enlargement of the sinusoidal vessels and abundant hepatocytes with pyknotic nuclei in the ACR group. Acrylamide toxicity resulted in elevated PlGF, accumulation of 8-hydroxydeoxyguanosine (8-OHdG), and increased Caspase-3 immunoreactivity in the liver. Oleuropein treatment reduced the increased expression of PlGF, 8-OHdG, and Caspase-3 against these deleterious effects observed in the ACR group. A positive correlation was observed between PlGF levels as well as oxidative stress and apoptosis markers in acrylamide toxicity. Oleuropein probably counteracted this mechanism by exhibiting antioxidant activity.
The Effect of N-Acetylcysteine Use on Endoplasmic Reticulum Stress in the Kidney Tissues of Obese Rats
(2023) TATAR, M.
Endoplasmic reticulum (ER) stress has recently acquired increasing importance in the pathogenesis of obesity-associated kidney disease. N-acetylcysteine, otherwise known as NAC, is an antioxidant that works directly and indirectly by increasing the production of antioxidants in cells. A diet consisting of 60% calories from fat was used to establish the obesity model for the present investigation. In the NAC and obesity + NAC (ObNAC) groups, NAC was administered by intragastric tube at 150 mg/kg for eight weeks. GRP78 and PERK expressions were determined immunohistochemically in sections collected from kidney tissues at the end of the experiment. The GRP78 H score was significantly higher in the obese group than in the control, NAC, and ObNAC groups (p<0.01). The ObNAC group H-score was significantly lower than that of the obese group (p < 0.01) but was not different from the control and NAC groups. The obese group PERK H-score was also significantly higher than the control, NAC, and ObNAC groups (p<0.01). In the ObNAC group, the H-score was significantly lower than that in the obese group (p<0.01) and significantly higher than those in the control and NAC groups (p<0.01). Increasing changes in stress markers may be improved by NAC application, since obesity induced by a high-fat diet activates ER stress in kidney tissue.