Browsing by Author "Sen F."

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An investigation on systematic history and distribution areas of tarek (Alburnus tarichi (güldenstädt, 1814))
(2021-01-01) Atici A.A.; Elp M.; Sen F.
This study aimed to determine the distribution area of tarek (Alburnus tarichi (Güldenstädt, 1814)) and to define how it must be called according to the scientific name by evaluating its previous studies made by various systematisers and their reports on its distribution, and by comparing literature knowledge. The taxonomic characteristics of the collected samples were evaluated and several measurements and counts were taken on the samples; analfin origin 0-4 scales behind dorsal fin-base; 72-91 lateral line scales; 21-29 gill rakers; 7½-10 branched dorsal-fin rays and 10-12½ branched anal-fin rays. Body and caudal peduncle was moderately compressed. Body was covered by overlapping scales. Tarek has five different populations in the Basin. They are Van, Erçek, Nazik and Aygır Lakes and Koçköprü Dam Lake populations. The main living area of the tarek population is Lake Van; however, between May and June, mature individuals of tarek enter to tributaries of Van Lake. Some of them are Karasu, Bendimahi, Deliçay, Zilan, Karmuç, Sapur, Yanıkçay, Gevaş, Engil, Kurubaş and Akköprü Streams. At the end of study, it was concluded that tarek should be called as Alburnus tarichi (Güldenstädt, 1814) and it is an endemic fish species distributed only in Van Lake Basin.
Analysis of DNA protection, interaction and antimicrobial activity of isatin derivatives
(2019-02-01) Ganim M.A.; Baloglu M.C.; Aygun A.; Altunoglu Y.C.; Sayiner H.S.; Kandemirli F.; Sen F.
Isatin, thiosemicarbazone and their derivatives have been widely used in biological applications such as antimicrobial, antiviral and anticancer therapies. Herein, eight isatin and thiosemicarbazone derivative compounds were re-synthesized and evaluated for DNA binding analysis including DNA protection studies using plasmid DNA (pUC19) and DNA interaction experiments using calf thymus DNA (CT-DNA). All compounds were also utilized in vitro assay to assess the antimicrobial activity of compounds against different pathogenic bacterial strains. All isatin and thiosemicarbazone derivative compounds exhibited DNA protection activity which ranged from 23.5 to 59.5%. Among them, I3-(N-2-MP)-TSC had the greatest DNA protective activity. For DNA binding analysis, all compounds had the same constant concentration (40 μM), which interacts with CT-DNA. It was also observed that DNA interactions gave a high intrinsic binding constant (Kb = 1.72 × 104 M−1–9.73 × 105 M−1). Besides, several derivatives of isatin thiosemicarbazone exhibited significant and selective antibacterial activity with low concentration. These compounds primarily affected Gram-positive bacteria, but were not effective against P. vulgaris and E. coli. The Gram-positive methicillin-resistant S. aureus ATCC 43300 (MRSA) was the most influenced strain by these compounds. It was found that methyphenyl group at isatin was essential for its antibacterial activity for MRSA.
Biogenic platinum nanoparticles using black cumin seed and their potential usage as antimicrobial and anticancer agent
(2020-02-05) Aygun A.; Gülbagca F.; Ozer L.Y.; Ustaoglu B.; Altunoglu Y.C.; Baloglu M.C.; Atalar M.N.; Alma M.H.; Sen F.
Herein, the biogenic platinum nanoparticles (Pt NPs) were synthesized by using black cumin seed (Nigella sativa L.) extract as a reducing agent. The biogenic platinum nanoparticles synthesized by black cumin seed extract was characterized in detail by Transmission Electron Microscopy (TEM), UV–vis spectrophotometer, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS). According to TEM analysis, Pt nanoparticles have spherical shapes and sizes between 1–6 nm. Moreover, the biogenic Pt NPs was assessed for its cytotoxicity effect on MDA-MB-231 breast and HeLa cervical cancer lines and their antibacterial effect against selected strains of gram-positive and negative bacteria. The cytotoxicity and bacterial tests showed the effectiveness of biogenic Pt nanoparticles. Dose-dependent toxicity effects were shown in the MDA-MB-231 breast and HeLa cervical cancer lines (IC50: 36.86 μg/mL and 19.83 μg/mL, respectively). In addition, Pt NPs showed high zone diameters against gram-positive and gram-negative bacteria at concentrations of 100 and 500 μg/ml. These results contribute to the development of the pharmaceutical industry as a potential antibacterial and anticancer agent.
Effects of Total Suspended Solids at Different Levels on the Eggs and Larvae of Endemic Fish, Tarek (Alburnus tarichi Güldenstädt, 1814) in the Karasu River (Van, Turkey)
(2023-01-01) Atici A.A.; Elp M.; Sen F.
Synergistic and Antagonistic Effects of Phenylalanine and Various Antibiotics on the Growth of Pathogenic Bacteria
(2019-06-15) Sen F.; Ganim M.; Baloglu M.; Aygun A.; Sayiner H.; Altunoglu Y.; Kandemirli F.; Demirkan B.; Kuyuldar E.; Bulut E.
Broad-spectrum antibiotics have been widely used in the treatment of many systemic and local infections in humans and animals. Herein, we aimed to determine the synergistic and antagonistic effects of phenylalanine with antibiotics cefoxitin, amoxicillin, vancomycin, lincomycin, and bacitracin against 14 pathogenic bacteria. The effect of antibiotics, either alone or in combination with this biomolecular liquid, was tested using the disk diffusion method against different bacteria. The addition of phenylalanine to antibiotic disks directly affected their antimicrobial activity. All the antibiotics used did not show any antimicrobial activity against Staphylococcus haemolyticus when used alone. However, in combination with phenylalanine, each antibiotic inhibited the growth of S. haemolyticus. The use of this biomolecular liquid together with amoxicillin and vancomycin also increased the antimicrobial activity against Enterococcus durans. The use of phenylalanine in combination with antibiotics also resulted in antagonistic effects on some pathogens. Further, the effects of antibiotics in combination with phenylalanine on different bacterial pathogens were investigated in vitro. Results provide valuable information to further our understanding of the molecular mechanism of action of antibiotics and to improve their efficacy against bacterial pathogens.
The effects of sand pits and sand extractions region on Karasu Stream (van) to water quality criteria
(2018-01-01) Atici A.; Elp M.; Sen F.
In this study, physical, chemical and microbiological parameters were analyzed in water samples in order to determine the effects of sand pits and sand extraction in Karasu Stream. The water quality data were collected monthly between June 2014 and May 20'6 from five sampling stations distributed along the stream and 39 parameters were examined such as dissolved oxygen, dissolved oxygen saturation, temperature, pH, electrical conductivity, flow, total suspended solids, turbidity, chlorine, calcium, magnesium, total hardness, carbonate, bicarbonate, total alkalinity, nitrate, nitrite, ammonium, ammonia, orthophosphate, total phosphor, sulfate, cyanide, silicon, silicon dioxide, biological oxygen demand, chemical oxygen demand, fluoride, fecal coliform, copper, aluminum, zinc, chromium, manganese, iron, molybdenum, cobalt, nickeland sediment accumulation. Turkish, EU and WHO water quality standarts were used in the evaluation of the results. Sand pits, sand extractions and the pollution caused by the settlement around the stream ruined the water quality of the stream and threatened the lives of the fish living in Karasu.