Browsing by Author "Sargin I."

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A new pollen-derived microcarrier for pantoprazole delivery
(2017-02-01) Akyuz L.; Sargin I.; Kaya M.; Ceter T.; Akata I.
Plant-derived carriers have emerged as promising materials for drug encapsulation. Especially, sporopollenin microcapsules extracted from diverse pollen species have been proved to be effective drug carriers due to their biocompatibility, homogeneity in size, resistance to harsh chemical conditions and high thermal stability. Here in this study, sporopollenin microcapsules were isolated successfully from the pollens of a common tree (Corylus avellana, the European hazelnut) and used as a carrier for pantoprazole (PaNa) (a proton pump inhibitor). The drug entrapment efficiency was recorded as 29.81%. SEM micrographs clearly showed the drug was loaded into the microcapsules through the apertures of microcapsule and also some drugs were adsorbed on the surface of microcapsules. FT-IR spectra analysis confirmed the drug loading. Thermogravimetric analysis revealed that thermal stability of PaNa was enhanced by encapsulation. In vitro release studies showed that PaNa-loaded sporopollenin microcapsules exhibited better release performance than the control. C. avellana sporopollenin microcapsules can make an efficient carrier for delivery of PaNa.
Controlled release and anti-proliferative effect of imatinib mesylate loaded sporopollenin microcapsules extracted from pollens of Betula pendula
(2017-12-01) Sargin I.; Akyuz L.; Kaya M.; Tan G.; Ceter T.; Yildirim K.; Ertosun S.; Aydin G.H.; Topal M.
Sporopollenin is a promising material for drug encapsulation due to its excellent properties; uniformity in size, non-toxicity, chemically and thermally resilient nature. Herein, morphologically intact sporopollenin microcapsules were extracted from Betula pendula pollens. Cancer therapeutic agent (imatinib mesylate) was loaded into the microcapsules. The encapsulation efficiency by passive loading technique was found to be 21.46%. Release behaviour of the drug from microcapsules was found to be biphasic, with an initial fast release followed by a slower rate of release. Imatinib mesylate release from the drug itself (control) was faster than from imatinib mesylate-loaded sporopollenin microcapsules. The release profiles for both free and entrapped drug samples were significantly slower and more controlled in PBS buffer (pH 7.4) than in HCl (pH 1.2) buffer. Cumulative drug release from IM-MES-loaded sporopollenin microcapsules was found to be 65% within 24 h for PBS, whereas release from the control was completed within 1 h. Also, a complete dissolution of control in HCl buffer was observed within first 30 min. MTT assay revealed that drug-loaded microcapsules were effective on WiDr human colon carcinoma cell line. B. pendula sporopollenin can be suggested as an effective carrier for oral delivery of imatinib mesylate.
Design and application of sporopollenin microcapsule supported palladium catalyst: Remarkably high turnover frequency and reusability in catalysis of biaryls
(2017-01-15) Baran T.; Sargin I.; Kaya M.; Menteş A.; Ceter T.
Bio-based catalyst support materials with high thermal and structural stability are desired for catalysts systems requiring harsh conditions. In this study, a thermally stable palladium catalyst (up to 440 °C) was designed from sporopollenin, which occurs naturally in the outer exine layer of pollens and is widely acknowledged as chemically very stable and inert biological material. Catalyst design procedure included (1) extraction of sporopollenin microcapsules from Betula pendula pollens (∼25 μm), (2) amino-functionalisation of the microcapsules, (3) Schiff base modification and (4) preparation of Pd(II) catalyst. The catalytic activity of the sporopollenin microcapsule supported palladium catalyst was tested in catalysis of biaryls by following a fast, simple and green microwave-assisted method. We recorded outstanding turnover number (TON: 40,000) and frequency (TOF: 400,000) for the catalyst in Suzuki coupling reactions. The catalyst proved to be reusable at least in eight cycles. The catalyst can be suggested for different catalyst systems due to its thermal and structural durability, reusability, inertness to air and its eco-friendly nature.
Incorporation of sporopollenin enhances acid–base durability, hydrophobicity, and mechanical, antifungal and antioxidant properties of chitosan films
(2017-03-25) Kaya M.; Akyuz L.; Sargin I.; Mujtaba M.; Salaberria A.M.; Labidi J.; Cakmak Y.S.; Koc B.; Baran T.; Ceter T.
Sporopollenin-chitosan blend films were produced for the first time. Sporopollenin is a robust structural component of plant pollens exhibiting excellent features such as nontoxicity, biodegradability, biocompatibility, high thermal stability, durability to strong acid and base solutions and homogeneity in size. To benefit from these advantages, sporopollenin samples obtained from Betula pendula (silver birch) were incorporated into chitosan film at different concentration; 10, 20 and 40 mg in 100 mL chitosan gel (1%). Stereo microscopy, FT-IR and TG/DTG analyses showed that sporopollenin was successfully incorporated into the chitosan matrix. Incorporation of sporopollenin in gradually increasing amount into chitosan films was found advantageous in (1) enhancement in chemical durability of the films, (2) increment of hydrophobicity, (3) boosting the mechanical properties, (4) improvement of antifungal and (5) antioxidant activities. This study revealed that sporopollenin can be suggested as an effective blend material for biodegradable edible chitosan film production.
Newly isolated sporopollenin microcages from Platanus orientalis pollens as a vehicle for controlled drug delivery
(2017-08-01) Mujtaba M.; Sargin I.; Akyuz L.; Ceter T.; Kaya M.
Sporopollenin microcages were produced from the pollens of Platanus orientalis. Paracetamol was loaded into the microcages. Pollen, sporopollenin, paracetamol and paracetamol-loaded sporopollenin microcages were characterized with FT-IR, TGA and SEM. The analytical analyses demonstrated that sporopollenin microcages were structurally intact, highly reticulated and thermally stable. The loading efficiency of the sporopollenin microcages was found to be 8.2% using the passive loading technique and 23.7% via evaporating loading technique. In vitro release and kinetics studies were performed to test the suitability of sporopollenin microcages for loading. These studies revealed that sporopollenin from P. orientalis can be suggested as a suitable carrier for drug loading and controlled release studies.
Preparation and characterisation of biodegradable pollen-chitosan microcapsules and its application in heavy metal removal
(2015-02-01) Sargin I.; Kaya M.; Arslan G.; Baran T.; Ceter T.
Biosorbents have been widely used in heavy metal removal. New resources should be exploited to develop more efficient biosorbents. This study reports the preparation of three novel chitosan microcapsules from pollens of three common, wind-pollinated plants (Acer negundo, Cupressus sempervirens and Populus nigra). The microcapsules were characterized (Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy and elemental analysis) and used in removal of heavy metal ions: Cd(II), Cr(III), Cu(II), Ni(II) and Zn(II). Their sorption capacities were compared to those of cross-linked chitosan beads without pollen grains. C. sempervirens-chitosan microcapsules exhibited better performance (Cd(II): 65.98; Cu(II): 67.10 and Zn(II): 49.55mgg-1) than the other microcapsules and the cross-linked beads. A. negundo-chitosan microcapsules were more efficient in Cr(III) (70.40mgg-1) removal. P. nigra-chitosan microcapsules were found to be less efficient. Chitosan-pollen microcapsules (except P. nigra-chitosan microcapsules) can be used in heavy metal removal.