Browsing by Author "Sarah AKAR"

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    Antimicrobial and anti-quorum sensing activities of giant fennel (Ferula elaeochytris Korovin) from the Hatay region
    (2020-03-01) Sarah AKAR; Nilay ÇÖPLÜ; Mehmet Cengiz BALOĞLU; Anfal Izaldeen ALKATEEB; Enis Fuat TÜFEKCI; Muammer KİRAZ; Orhan CORUM; Yasemin Celik ALTUNOĞLU
    Aim: Today, increasing antibiotic resistance has focused the science world onthe discovery of new antimicrobial molecules or the development of alternative methods of struggle. One of the alternative methods is thought to be inhibition of bacterial quorum sensing (QS). Because the QS system performs acrucial part in the synthesis of virulence factors in bacteria. Numerous medicinal plants are known to have promising antimicrobial and anti-QS activities.One of these plants may be giant fennel (Ferula elaeochytris Korovin), whichhas been extensively used in Anatolia for years. Therefore, it was aimed to investigate the antimicrobial and anti-QS activities of the root extract of F. elaeochytris Korovin in this study. .Materials and Methods: The antimicrobial activity of the extract was screened by disc diffusion assay against various microorganisms. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) ofthe extract against sensitive strain(s) were determined using broth microdilution assay. Anti-QS activity investigated on inhibition of violacein and pyocyanin production in Chromobacterium violaceum and Pseudomonas aeruginosabioreporter strains, respectively.Results: The extract exhibited the antimicrobial activity against only Staphylococcus aureus (zone of inhibition (ZOI) = 9.3±0.6 mm). The MIC and theMBC values were determined as 4.4±1.9 mg/mL and >105 mg/mL, respectively. No inhibition of violacein production was detected. Pyocyanin productionwas reduced by 60% and 82% at sub-MIC concentrations (at 1.64 mg/mL and3.28 mg/mL, respectively) as against the control (p<0.05) without suppressing bacterial growth.Conclusion: This study shows that the root extract of F. elaeochytris Korovinmay be a good candidate to develop antistaphylococcal and anti-QS agents.
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    Determination of PER-1 and OXA-10-like ExtendedSpectrum Beta-Lactamases Frequency in CeftazidimeResistant Pseudomonas aeruginosa Strains Isolated in Kastamonu Training and Research Hospital, Turkey
    (2022-03-01) Sarah AKAR; Mehmet Cengiz BALOĞLU; Nilay ÇÖPLÜ; Yasemin ÇELİK ALTUNOĞLU; Enis Fuat TÜFEKCİ; Çetin KILINÇ
    Introduction: Detecting Pseudomonas aeruginosa strains producing extended-spectrum beta-lactamase (ESBL) in the hospital setting and taking necessary precautions against them is important for infection control and public health. This study aimed to investigate PER-1 and OXA-10-like ESBLs production frequency in ceftazidime-resistant P. aeruginosa obtained from Kastamonu Training and Research Hospital. Materials and Methods: Forty-two ceftazidime-resistant P. aeruginosa strains from different patients between April 2018 and March 2020 were included in the study. Identification of the strains and antibiotic susceptibility tests were studied according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria by VITEK 2 Compact automated system (BioMerieux, France). ESBL production of the strains was studied by combined disk test, phenotypically. The presence of PER-1 and OXA-10-like genes was investigated by polymerase chain reaction (PCR). Confirmation of the PCR product was done using DNA sequencing on an ABI PRISM 3130XL Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). Results: The resistance rates of the strains were: 19%, 81%, 88%, 88%, 91%, 95%, and 95% for amikacin, gentamicin, meropenem, cefepime, imipenem, piperacillin-tazobactam, and ciprofloxacin, respectively. Twenty-seven (64%) strains had the ESBL phenotype. Only one strain had PER-1 gene. OXA-10-like gene was not found in any strains. PER-1 sequence was identical and corresponded to the published sequences for PER-1 gene in GenBank at the National Center for Biotechnology Information. Conclusion: These results showed that PER-1 and OXA-10-like genes were not common among ceftazidime-resistant P. aeruginosa strains obtained from our hospital. ESBL production can be determined phenotypically in ceftazidime-resistant P. aeruginosa strains. However, confirming the results with molecular tests is significant for epidemiological studies.