Browsing by Author "Samli H."

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A case with mosaic ring chromosome 18
(2013-11-15) Samli H.; Özgöz A.; Içduygu F.; Hekimler K.; Sivaci Y.; Imirzalioǧlu N.
The classical mode of ring chromosome formation is by break forming in both arms of the affected chromosome, fusion of the breaking points and loss of the distal fragments. Ring chromosome of the chromosome 18 is relatively common among ring chromosomes and the rate of having typical clinical sings of 18p and 18q sydromes vary related to the length of the deletion in 18p and 18q. Ring 18 phenotype is characterised by growth retardation, mental retardation and nonspecific abnormalities, also facial dysmorphism and malformations may be observed. Our case referred with congenital malformation, motor mental retardation (MMR), short stature, high palate, pectus excavatus was evaluated genetically. GTL banding and FISH methods were performed for the metaphase plaques obtained from peripheral lymphocytes cultered for 72 hours. The karyotype of the case was detected to be 46,XX,r(18)[25]/46,XX[75] and confirmed by FISH analysis. © Copyright 2013 by Gazi University Medical Faculty.
Possibility of paclitaxel to induce the stemness-related characteristics of prostate cancer cells
(2021-10-01) Içduygu F.M.; Samli H.; Özgöz A.; Vatansever B.; Oztürk K.H.; Akgün E.
Background. Drug resistance poses a crucial problem in the treatment of prostate cancer. Recent studies have shown that chemotherapy agents may cause cancer cells to acquire stem cell-like properties, resulting in drug resistance and, eventually, treatment failure. Objectives. To evaluate whether long-term paclitaxel exposure causes an increase in the stem cell-like properties of prostate cancer cells. Materials and methods. Paclitaxel-resistant PC-3 cells were generated from parental PC-3 cells by treating them with increasing concentrations of paclitaxel. The expression levels of the stem cell markers NANOG, C-MYC, CD44, and ABCG2 were evaluated using quantitative real-time polymerase chain reaction (RT-qPCR). A sphere formation assay was performed to test the potential of the cells to behave as stem cells, and a wound healing assay was carried out to evaluate migration ability of the cells. Results. The expression levels of C-MYC and NANOG were significantly higher in paclitaxel-resistant PC-3 cells compared to the parental PC-3 cells. However, there was no significant increase in the expression of CD44 or ABCG2. In addition, the sphere-forming capacity and migration ability of resistant PC-3 cells were increased. Conclusions. The results of the current study indicate that paclitaxel exposure may increase the stem cell-like properties of PC-3 prostate cancer cells.