Browsing by Author "Orhan CORUM"

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    Antimicrobial and anti-quorum sensing activities of giant fennel (Ferula elaeochytris Korovin) from the Hatay region
    (2020-03-01) Sarah AKAR; Nilay ÇÖPLÜ; Mehmet Cengiz BALOĞLU; Anfal Izaldeen ALKATEEB; Enis Fuat TÜFEKCI; Muammer KİRAZ; Orhan CORUM; Yasemin Celik ALTUNOĞLU
    Aim: Today, increasing antibiotic resistance has focused the science world onthe discovery of new antimicrobial molecules or the development of alternative methods of struggle. One of the alternative methods is thought to be inhibition of bacterial quorum sensing (QS). Because the QS system performs acrucial part in the synthesis of virulence factors in bacteria. Numerous medicinal plants are known to have promising antimicrobial and anti-QS activities.One of these plants may be giant fennel (Ferula elaeochytris Korovin), whichhas been extensively used in Anatolia for years. Therefore, it was aimed to investigate the antimicrobial and anti-QS activities of the root extract of F. elaeochytris Korovin in this study. .Materials and Methods: The antimicrobial activity of the extract was screened by disc diffusion assay against various microorganisms. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) ofthe extract against sensitive strain(s) were determined using broth microdilution assay. Anti-QS activity investigated on inhibition of violacein and pyocyanin production in Chromobacterium violaceum and Pseudomonas aeruginosabioreporter strains, respectively.Results: The extract exhibited the antimicrobial activity against only Staphylococcus aureus (zone of inhibition (ZOI) = 9.3±0.6 mm). The MIC and theMBC values were determined as 4.4±1.9 mg/mL and >105 mg/mL, respectively. No inhibition of violacein production was detected. Pyocyanin productionwas reduced by 60% and 82% at sub-MIC concentrations (at 1.64 mg/mL and3.28 mg/mL, respectively) as against the control (p<0.05) without suppressing bacterial growth.Conclusion: This study shows that the root extract of F. elaeochytris Korovinmay be a good candidate to develop antistaphylococcal and anti-QS agents.