Browsing by Author "Ocal M."

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A broad-spectrum biological activities of Heracleum humile extracts: A first report of the antiviral, anti-cancer and chemical properties
(2024-12-01) Ocal M.; Duran Yelken S.; Altunoglu Y.C.; Baloglu M.C.; Soomro S.I.; Zengin G.; Angeloni S.; Mustafa A.M.; Caprioli G.; Paksoy M.Y.
Comparative Content, Biological and Anticancer Activities of Heracleum humile Extracts Obtained by Ultrasound-Assisted Extraction Method
(2022-07-01) Ocal M.; Altunoglu Y.C.; Angeloni S.; Mustafa A.M.; Caprioli G.; Zengin G.; Paksoy M.Y.; Baloglu M.C.
As the safety and effectiveness of synthetic drugs remain in doubt, researchers are trying to develop natural medicines from medicinal plants. Herein, ethyl acetate, methanol and water extracts from the Heracleum humile plant were obtained by an ultrasonic-assisted extraction process and the aim was to evaluate some biological effects of the extracts due to the limited data on the pharmacological properties of Heracleum humile in the literature. Weak antibacterial activity was observed on tested bacterial species. The minimum inhibitory concentration and the minimum bactericidal concentration values ranged from 250 to 500 μg/mL. In addition, cytotoxic activity was determined using the MTT test. The strongest findings were determined for ethyl acetate extract on the MDA-MB-231 cell lines at the 48th hour (IC50:97.94 μg/mL), followed by the MCF-7 cell lines at the 24th hour (IC50:103.9 μg/mL). All extracts of Heracleum humile contained mainly flavonoids, phenolic acids and their derivatives, i. e., well-known compounds that possess numerous biological activities such as antioxidant, anti-inflammatory, anticancer, antimicrobial etc. The study results could provide important information that Heracleum humile could be a potential candidate as a natural enzyme inhibitor. It can be concluded that these extracts could be useful in the elementary step of improving novel plant-derived multifunctional pharmaceuticals.
Integration of in vitro and in silico approaches to assess three Astragalus species from Turkey flora: A novel spotlight from lab bench to functional applications
(2022-10-01) Zengin G.; Uba A.I.; Ocal M.; Sharifi-Rad M.; Caprioli G.; Angeloni S.; Altunoglu Y.C.; Baloglu M.C.; Yıldıztugay E.
Members of the genus Astragalus have a great interest as a source of natural bioactive compounds on a scientific platform. To provide multidirectional insights into three Astragalus species (A. setulosus, A. anthylloides, and A. ovalis), the current work focused on the chemical characterization and biological properties of their extracts (aerial parts and roots). The chemical characterization of the extracts was detected by HPLC-MS/MS analysis. The biological properties were evaluated by antioxidant, enzyme inhibitory, and cytotoxic parameters. Assays for radical quenching, reducing capacity, and metal chelation were also used to evaluate antioxidant properties. To test the enzyme inhibitory effects of the extracts, cholinesterases, tyrosinase, α-amylase, and α-glucosidase were utilized as target enzymes. Two cancer cell lines, (MCF-7 (human breast cancer cell line) and HeLa (Human cervix cancer cell line), were selected to evaluate cytotoxic effects. Generally, 5- caffeoylquinic acid (2.43–283.92 μg/g extract), hyperoside (4.33–216.22 μg/g extract) and rutin (1.09–184.98 μg/g extract) were the main constituents. The extracts from aerial parts and roots of A. anthylloides showed stronger radical scavenging and reducing power abilities compared to A. setulosus and A. ovalis. The best AChE and BChE inhibitory effects were determined in the aerial parts of A. setulosus (2.18 mg GALAE/g) and roots of A. ovalis (4.76 mg GALAE/g), respectively. The extracts of A. ovalis had the highest tyrosinase inhibitory abilities. The extract from aerial parts of A. setulosus showed stronger cytotoxic effects compared to other extracts. Pearson's correlation analysis revealed that the presence of some compounds (resveratrol, p-coumaric, 5-caffeoylquinic, and ferulic acids, etc) was linked to the observed biological activities. Molecular docking was also provided for the possible interaction of enzymes as well as protein targets of the tested cell lines. Our findings provide a scientific basis for the Astragalus species, which may serve as a source of naturally occurring bioactive compounds for health-promoting applications.
Multidirectional insights on Chrysophyllum perpulchrum leaves and stem bark extracts: HPLC-ESI-MS ⁿ profiles, antioxidant, enzyme inhibitory, antimicrobial and cytotoxic properties
(2019-08-01) Baloglu M.C.; Llorent-Martínez E.J.; Aumeeruddy M.Z.; Mahomoodally M.F.; Altunoglu Y.C.; Ustaoglu B.; Ocal M.; Gürel S.; Bene K.; Sinan K.I.; Zengin G.
Chrysophyllum perpulchrum Mildbr. ex Hutch. & Dalziel has been reported to possess several therapeutic properties in African traditional medicine. However, its pharmacological properties have not been fully studied. Herein, we focused on the evaluation of the antioxidant, enzyme inhibitory, antimicrobial and cytotoxic effects of three solvent extracts (ethyl acetate, methanol, and water) of the stem bark and leaves. Polyphenolic components of the extracts were also identified by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MS n) . The methanolic stem bark extract possess the highest DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging, ferric and molybdenum (VI) reducing, and the highest inhibitory effect against acetylcholinesterase and butyrylcholinesterase. The aqueous stem bark extract displayed the highest ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) scavenging and cupric reducing power. In contrast, the leaf ethyl acetate extract was the most effective metal chelator and α-amylase inhibitor while the leaf methanolic extract exhibited the highest tyrosinase and α-glucosidase inhibition. The highest total phenolic content (131.58 mg GAE/g extract) was recorded from the methanolic stem bark extract while the leaf methanolic extract was the richest in total flavonoid content (30.43 mg RE/g extract). Flavan-3-ols were main group in the methanol extracts. The minimum inhibitiory concentration values for the methanolic stem bark extract against Gram positive and negative bacteria strains ranged between 62.5–125 μg/ml. Bark extracts of C. perpulchrum were very effective against Salmonella kentucky, Proteus vulgaris and Staphylococcus aureus at 62.5 μg/ml. In addition, cytotoxic activity of stem bark samples was evident at lower concentration than those of leaf extracts. Stem bark extracts displayed the highest prevention against to HeLa cell line (IC 50= 264 μg/mL after 48 h). In light of the above, C. perpulchrum might provide health benefits against oxidative stress and the associated chronic diseases. Nonetheless, the detailed mechanism of action is yet to be further investigated.