Browsing by Author "Musa KAVAS"

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    Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings
    (2013-11-01) Mehmet Cengiz BALOĞLU; Musa KAVAS; Fatma Selin KÖSE; Derya GÖKÇAY; Oya AKÇA
    Drought stress is one of the most serious environmental limitations affecting the growth and productivity of plants. In the current study, oxidative damage and antioxidant responses under polyethylene glycol (PEG)-induced osmotic stress were compared in 2 melon cultivars, Kırkağac and Galia. Melon seedlings were subjected to PEG-6000 solutions of 2 different osmotic potentials, 0.2 MPa and 0.4 MPa. Various physiological parameters, malondialdehyde (MDA), proline content, and antioxidant enzymes including catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were measured. A reduction in fresh and dry weights of shoot and root tissues was observed. Significant proline accumulation was detected with increasing osmotic potential for both cultivars. A significant rise in MDA was detected in Kırkağac at 0.4 MPa osmotic potential. In Galia hydrogen peroxide ($H_{2}O_{2}$) content increased significantly as PEG concentration increased. CAT showed significantly increased activity only at 0.4 MPa osmotic potential in both cultivars. PEG-induced osmotic stress altered GR activity in both cultivars. These results suggest that (i) Galia is more tolerant than Kırkağac, and (ii) drought tolerance in both cultivars might be closely related to an increase in capacity for antioxidant enzyme activity and the osmoprotective function of proline. Comparing these responses will help to identify drought tolerance mechanisms in melon cultivars.
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    TRDizin
    Enhanced salt tolerance of transgenic tobacco expressing a wheat salt tolerance gene
    (2016-02-01) Musa KAVAS; Ayşe Meral YÜCEL; Hüseyin Avni ÖRTEM; Mehmet Cengiz BALOĞLU
    Soil salinity is one of the most important limiting factors of agricultural productivity in the world. The Triticum aestivum salt tolerance-related gene (TaSTRG) possesses a functional response to salt and drought stress conditions. A variety of stress factors, such as salt, drought, abscisic acid, and cold, may induce the expression of TaSTRG in wheat. In this study, the TaSTRG gene was transferred to tobacco via Agrobacterium-mediated transformation. Overexpression of TaSTRG in transgenic tobacco plants indicated higher salt tolerance and mediated more vigorous growth than in wild-type plants. Under salt stress conditions, the transgenic tobacco plants had higher germination and survival rates and longer root length than the control plants. Under salt treatments (200-250 mM), TaSTRG-overexpressing tobacco plants accumulated a higher amount of proline and had significantly lower malondialdehyde content than wild-type plants. Furthermore, transgene inheritance followed Mendelian laws, indicating the stability of TaSTRG in transgenic tobacco plants. These results indicated that the wheat TaSTRG gene plays an important role in responding to salt stress.