Browsing by Author "Mujtaba M."

Now showing 1 - 4 of 4

Chitosan Loses Innate Beneficial Properties after Being Dissolved in Acetic Acid: Supported by Detailed Molecular Modeling
(2020-12-14) Bilican I.; Pekdemir S.; Onses M.S.; Akyuz L.; Altuner E.M.; Koc-Bilican B.; Zang L.S.; Mujtaba M.; Mulerčikas P.; Kaya M.
Chitosan, which is obtained via deacetylation of chitin, has a variety of uses in agriculture, food, medicine, pharmaceuticals, and cosmetics. Industrial chitosan is in a gel form, which is produced by dissolving in acetic acids. These gels can be chitosan-only films or composite films that include other ingredients such as plant extracts or other polymers. Chitosan-based films, however, are not as natural as chitosan dissolved in weak acids, and they lack some of chitosan's innate properties. In this study, natural chitosan films (NCFs) were obtained from the pupa shells of black soldier flies through a process that maintains the original structure. The semisynthetic film (SCF) was then produced by dissolving the same NCF in acetic acid along with glycerol and glutaraldehyde. The semisynthetic film remarkably lost the beneficial properties of the natural film. The deteriorated characteristics include hydrophobicity, crystallinity, thermal properties, as well as a loss of fibril structure and a reduction in bacterial attachment. Moreover, the Ag-deposited NCFs manifested strikingly higher surface-enhanced Raman scattering activity as compared with the semisynthetic ones. These results, including the molecular modeling data, demonstrate that dissolving chitosan in acetic acid changes its polymeric structure.
Incorporation of sporopollenin enhances acid–base durability, hydrophobicity, and mechanical, antifungal and antioxidant properties of chitosan films
(2017-03-25) Kaya M.; Akyuz L.; Sargin I.; Mujtaba M.; Salaberria A.M.; Labidi J.; Cakmak Y.S.; Koc B.; Baran T.; Ceter T.
Sporopollenin-chitosan blend films were produced for the first time. Sporopollenin is a robust structural component of plant pollens exhibiting excellent features such as nontoxicity, biodegradability, biocompatibility, high thermal stability, durability to strong acid and base solutions and homogeneity in size. To benefit from these advantages, sporopollenin samples obtained from Betula pendula (silver birch) were incorporated into chitosan film at different concentration; 10, 20 and 40 mg in 100 mL chitosan gel (1%). Stereo microscopy, FT-IR and TG/DTG analyses showed that sporopollenin was successfully incorporated into the chitosan matrix. Incorporation of sporopollenin in gradually increasing amount into chitosan films was found advantageous in (1) enhancement in chemical durability of the films, (2) increment of hydrophobicity, (3) boosting the mechanical properties, (4) improvement of antifungal and (5) antioxidant activities. This study revealed that sporopollenin can be suggested as an effective blend material for biodegradable edible chitosan film production.
Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay
(2022-01-01) Mujtaba M.; Yilmaz B.A.; Cansaran-Duman D.; Akyuz L.; Yangın S.; Kaya M.; Çeter T.; Khawar K.M.
Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.
Newly isolated sporopollenin microcages from Platanus orientalis pollens as a vehicle for controlled drug delivery
(2017-08-01) Mujtaba M.; Sargin I.; Akyuz L.; Ceter T.; Kaya M.
Sporopollenin microcages were produced from the pollens of Platanus orientalis. Paracetamol was loaded into the microcages. Pollen, sporopollenin, paracetamol and paracetamol-loaded sporopollenin microcages were characterized with FT-IR, TGA and SEM. The analytical analyses demonstrated that sporopollenin microcages were structurally intact, highly reticulated and thermally stable. The loading efficiency of the sporopollenin microcages was found to be 8.2% using the passive loading technique and 23.7% via evaporating loading technique. In vitro release and kinetics studies were performed to test the suitability of sporopollenin microcages for loading. These studies revealed that sporopollenin from P. orientalis can be suggested as a suitable carrier for drug loading and controlled release studies.