Browsing by Author "Mahmut GÜR"

Now showing 1 - 5 of 5

Chemical Composition and Antimicrobial Activities of Cold-Pressed Oils Obtained From Nettle, Radish and Pomegranate Seeds
(2018-12-01) Sana I. SOULMAN; Hana Ealoma AKWIETEN; Kerim GÜNEY; Bayram KIRAN; Mahmut GÜR; Ergin Murat ALTUNER; Talip ÇETER
Aim of study: The aim of this study is to put forward the antimicrobial activity of cold pressed oils obtained from seeds of nettle (Urtica dioica), radish (Raphanus sativus) and pomegranate (Punica granatum).Material and Methods: Oils of these seeds were analysed for their antibacterial and antifungal activities by the disk diffusion and MIC tests against fifteen microorganisms, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus ATCC 25923, Salmonella typhimurium SL 1344, Salmonella kentucky, Salmonella infantis, Salmonella enteritidis, Pseudomonas fluorescens P1 ATCC 13075, Pseudomonas aeruginosa DSMZ 50071, Klebsiella pneumoniae, Escherichia coli ATCC 25922, Enterococcus faecium, Enterococcus faecalis ATCC 29212, Enterobacter aerogenes ATCC 13048, Candida albicans DSMZ 1386 and Bacillus subtilis DSMZ 1971. The results were compared against 11 standard antibiotics, which are cefazolin, clindamycin, chloramphenicol, ciprofloxacin, amoxicillin/clavulanic acid, sulfamethoxazole/trimethoprim, ceftriaxone, gentamicin, ampicillin, cephalothin, cefuroxime and vancomycin. The extracts were also chemically analysed by using GC-MS.Main results: As a result, radish oil is observed to be active against all microorganisms with the highest activity, where nettle oil is active against all microorganisms except for S. epidermidis. The lowest activity was observed in pomegranate oil.Highlights: The results of the study clearly puts forward that oils obtained from nettle, radish and pomegranate could have a possible medicinal use.
Chemical composition and antimicrobial activity of Myristica fragrans & Elettaria cardamomum essential oil
(2018-12-01) Betül GÜNEY; Osman Emre ÖZKAN; Kerim GÜNEY; Saim ATEŞ; Çağrı OLGUN; Mahmut GÜR
Aim of study: The objectives in this study were to determine the chemical composition and antimicrobial activity of the essential oils from nutmeg (Myristica fragrans) and cardamom (Elettaria cardamomum).Material and Methods: The essential oil was obtained from nutmeg and cardamom seeds through hydrodistillation method. The chemical composition of nutmeg and cardamom essential oil was analyzed by GC-MS. The antimicrobial activity of essential oil was investigated against Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterococcus faecalis ATCC 29212, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae , Listeria monocytogenes, Listeria innocua, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13076, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis DSMZ 20044 with minimum inhibitory concentration (MIC) method.Main results: GC-MS analyzes shows that γ-terpinene (19.16%), (-)-terpinen-4-ol (14.08%), α-pinene (6.75%), myristicin (6.18%), β-pinene (4.85%), sylvestrene (4.72%), elemicin (4.68%) and safrole (4.17%) identified in nutmeg essential oil. Also, α-terpinyl acetate (34.55%), eucalyptol (24.91%), (-)-terpinen-4-ol (10.63%), linalool (7.69%) and neryl butyrate (6.58%) compounds identified in cardamom essential oil as major compounds. Nutmeg and cardamom essential oil showed an antimicrobial activity against almost all test microorganisms.Research highlights: This study suggests that nutmeg and cardamom essential oil may be useful in the medicinal application and food industry as a preservative.
Chemical Composition, Antibacterial and Antioxidant Activities of Thymus Praecox
(2021-03-01) Pınar BALOĞLU; Kerim GÜNEY; İzzet ŞENER; Fulya TEKELİOĞLU; Merve ZURNACI; Mahmut GÜR
Aim of Study: It was aimed to determine the chemical composition, antioxidant and antibacterial activityof the Thymus praecox which distributed in the Kastamonu region.Material and methods: Spectroscopic and chromotographic analysis were applied in the determinationof the chemical composition. Thyme extracts were prepared using five different solvents. In Thymuspraecox, the presence of flavonoids using HPLC and the chemical content of essential oil using GC-MSwere investigated. The analyses of the mineral were determined in leaf and stem of thyme by ICP-OES.Antioxidant capacity was determined using two methods such as DPPH free radical scavenging and H2O2scavenging. Antibacterial activity on ten bacteria, Gram (+) and Gram (-) was tested using the disc diffusionmethod.Main results: Experimental results showed that thyme extracts have antibacterial activities against somebacteria. As a result, it was found that the most affected bacteria was S. aureus. Inhibition zone diameterwas determined to be between 8-12 mm. The effect of solvent on antibacterial activity, antioxidant capacity,total phenolic and total flavonoid amounts were observed.Highlights: These results showed that T. praecox has the potential to be used as a natural antimicrobialand antioxidant agent, and can be used as a natural supportive treatment.
Novel 1,3,4-thiadiazole compounds derived from 4-phenylbutyric acid: Synthesis, characterization and DFT studies
(2018-06-01) Nesrin ŞENER; Mahmut GÜR
In this study; new 1,3,4-thiadiazole compounds were synthesized and their structural characterization were carried out by using FT-IR, 1H-NMR, 13C-NMR spectroscopic methods, and elemental analysis. Furthermore, the absorption properties of the synthesized compounds were also studied with UV-Vis. spectrophotometer. In addition, molecular conformation, electronic properties and UV-vis analysis of synthesized 1,3,4-thiadiazole compounds were theoretically investigated by using density functional theory (DFT), and how F, Cl and methoxy isomers affected the molecular configuration was analyzed.
The Structure-Activity Relationships of Familiar Antiepileptic Drugs and Na+ Channels
(2022-06-30) Esra Nur ÇAKMAK; Mahmut GÜR; Bayram KIRAN
The aim of this study is to examine the effects of drug active compounds, which are widely used in the treatment of epilepsy, on voltage-gated Na+ channels are important channels that advance the action potential in the excitation direction by molecular docking method. These molecules have been selected considering the physiopathological effect mechanisms of epilepsy disease. When the action potential is stimulated, Na+ channels allow sodium ion entry into the cell and cause epilepsy seizures. For this reason, PDB ID: 4PA6 receptor, which acts as an antagonist according to its activity on the canal in the formation of epileptic seizures, was chosen for molecular docking study. As a result of molecular docking studies; Phenytoin gave the best binding affinity for 4PA6 with a value of -7.7 kcal/mol. Other results in descending order (as kcal/mol); Mesuximide (-7.5), Remacemide (-7.3), Tiagabine (-7.1), Ethotoin and Mephenytoin (-7.0), Primidone (-6.9), Topiramate (-6.6), Oxcarbazepine and Lamotrigine (-6.3), Felbamate (-6.0), Lacosamide (-5.9), Zonisamide (-5.8), Levetirecetam and Gabapentin (-5.7), Ethosuximide (-5.6), Trimethadion (-5.1), Valproic Acid (-5.0), Vigabatrin (-4.0), determined as.