Browsing by Author "Kutlu B."

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A comparative study on the effects of lead and zeolite on serum biochemistry of carp fish, Cyprinus carpio Linnaeus, 1758 (Cyprinidae)
(2015-01-01) Mutlu E.; Demir T.; Kutlu B.
One of the pollutants leading to heavy metal pollution is the lead element. The studies on exposure of fish to lead have shown that this exposure leads to negative effects on body weight, and digestive enzymes and lipase. In humans, the symptoms of chronic lead toxicity are renal malfunction, anemia, brain and liver damage, cancer, hyperactivity, and general psychological impairment. For struggling with this heavy metal contamination, we have a useful tool named "Zeolite". Zeolites are used in many fields such as agriculture, metal industry, energy sector, manufacturing industry, water treatment, animal husbandry, struggling with environmental pollution, and etc. In this study, we utilized the biochemical parameters of carp fish (Cyprinus carpio) in order to reveal the effects of zeolite on fish health and production. The experiments were performed within 100 L glass aquariums with 85×40×30 dimensions. The study was planned for 3 different durations as 35 days, 90 days, and 120 days. The subjects were divided into 4 groups for each of experiment durations. The groups were named Pb(NC)3)2 group, Pb(NO3)2 + Zeolite group, Zeolite group, and control group. At the end of each of the experiment period (on 35th, 90th, and 120th days), the fish in groups were weighed, and the average growth rates of each of the groups were calculated. The parameters used in blood analyses are glucose (GLU), blood urea nitrogen (BUN), total protein (TP), albumin (ALB), globulin (GLB), creatinine (CR), total bilirubin (TBIL), uric acid (UA), and direct bilirubin (DBIL).
Alterations of growth performance and blood chemistry in nile tilapia (Oreochromis nuoticus) affected by copper sulfate in long-term exposure
(2015-09-08) Mutlu E.; Aydın S.; Kutlu B.
The objective of this study was to evaluate the effects of copper sulfate (CuSO4.(5 H2O)) on the Nile tilapia (Oreochromis niloticus). Median (LC50) and minimum (LC1O) lethal concentrations of copper sulfate on Nile tilapia were determined via acute tests. LC50 values of copper sulfate for the 48th, 72nd and 96th hours were 13.15, 12.95 and 12.85 mg/L, respectively. LC10 values of 12, 11 and 9.5 mg/L were obtained for similar exposure periods (48th, 72nd and 96th hours), respectively. The effects of 1.5 mg/L dose of copper sulfate on growth rate, gross clinical observations and blood biochemical parameters of Nile tilapia were evaluated after 35, 65 and 95 days of exposure periods. The differences of growth rates between control and copper sulfate-exposed groups were not significant (P>0.05) at the end of 35, 65 and 95 days. Continuous exposure of Nile tilapia to 1.5 mg/L concentration of copper sulfate in water solution for three periods (35, 65 and 95 days) decreased the total protein, albumin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose (except the elevation in the exposed group at the end of 95 days period), magnesium (Mg+²), calcium (Ca+²), iron (Fe+²), potassium (K+), sodium (Na+1), chloride (CI-) and phosphate values in serum. The cholesterol, low density lipid (LDL), triglyceride, globulin, creatinine, alkaline phosphatase (ALP), total bilirubin, direct bilirubin, uric acid and blood urea nitrogen (BUN) concentrations in the serum increased in copper sulfate-exposed fish.
Growth and bioaccumulation of cadmium, zinc, lead, copper in Dunaliella sp. isolated from Homa lagoon, eastern Aegean Sea
(2017-01-01) Kutlu B.; Mutlu E.
Present study consists the growth of cell and bioaccumulation capacity of Cadmium (Cd), Zinc (Zn), Lead (Pb), Copper (Cu) by Dunaliella sp. isolated from Homa Lagoon, Eastern Aegean, Turkey. Sterile medium f/2 was used for the cultivation. Growth rate, mean growth rate, relative growth rate and generation time of Dunaliella sp. were calculated for determining the growth of cell. In addition, optimum pH values were determined for each heavy metal. Our results pointed out that the optimum time for harvesting Dunaliella sp. was in 8 days. Uptake of heavy metals of Dunaliella sp. showed high tendency for zinc accumulation followed by copper, lead, and the lowest tendency was for cadmium. Highest bioaccumulation of Zn was determined to be 151.64 µg/L at 2.45 of pH under in situ conditions. pH had noticeably affected the bioaccumulation capacity of Dunaliella sp.